(Linear) (Six-base) MAPSORT of: KIEE0401.seq  Check: 1168  from: 1  to: 615



KIEE0401.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     320     615 
   Size:        151     169     295

  Fragments arranged by size: 

                 295     169     151
 
AleI CACnn'nnGTG

Cuts at:      0      54     615 
   Size:         54     561
 
BamHI G'GATC_C

Cuts at:      0       9     615 
   Size:          9     606
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     546     615 
   Size:        546      69
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      24     163     615 
   Size:         24     139     452

  Fragments arranged by size: 

                 452     139      24
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     334     537     615 
   Size:        334     203      78

  Fragments arranged by size: 

                 334     203      78
 
BsaAI yAC'GTr

Cuts at:      0     274     615 
   Size:        274     341
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     238     268     615 
   Size:        238      30     347

  Fragments arranged by size: 

                 347     238      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     615 
   Size:        193     422
 
BspHI T'CATG_A

Cuts at:      0     209     615 
   Size:        209     406
 
BsrDI GCAATG_nn'

Cuts at:      0      74     615 
   Size:         74     541
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      37     615 
   Size:         37     578
 
BstYI r'GATC_y

Cuts at:      0       9      68     615 
   Size:          9      59     547

  Fragments arranged by size: 

                 547      59       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     422     615 
   Size:        422     193
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     320     334     537     615 
   Size:        151     169      14     203      78

  Fragments arranged by size: 

                 203     169     151      78      14
 
EcoO109I rG'GnC_Cy

Cuts at:      0     408     615 
   Size:        408     207
 
HincII GTy'rAC

Cuts at:      0     467     615 
   Size:        467     148
 
MslI CAynn'nnrTG

Cuts at:      0      54     615 
   Size:         54     561
 
MspA1I CmG'CkG

Cuts at:      0     264     615 
   Size:        264     351
 
NspI r_CATG'y

Cuts at:      0      63     615 
   Size:         63     552
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48     615 
   Size:         16      32     567

  Fragments arranged by size: 

                 567      32      16
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     527     559     615 
   Size:        527      32      56

  Fragments arranged by size: 

                 527      56      32
 
PvuII CAG'CTG

Cuts at:      0     264     615 
   Size:        264     351
 
SapI GCTCTTCn'nnn_

Cuts at:      0     422     615 
   Size:        422     193
 
SfcI C'TryA_G

Cuts at:      0     562     615 
   Size:        562      53
 
SnaBI TAC'GTA

Cuts at:      0     274     615 
   Size:        274     341
 
SpeI A'CTAG_T

Cuts at:      0     511     615 
   Size:        511     104
 
SphI G_CATG'C

Cuts at:      0      63     615 
   Size:         63     552
 
TatI w'GTAC_w

Cuts at:      0     380     397     615 
   Size:        380      17     218

  Fragments arranged by size: 

                 380     218      17
 
XmnI GAAnn'nnTTC

Cuts at:      0     338     615 
   Size:        338     277
 

 Enzymes that do cut:

     AcuI     AleI    BamHI    BciVI     BmrI     BpmI    BsaAI    BsaXI
     BsgI    BspHI    BsrDI    BstXI    BstYI     EarI  Eco57MI EcoO109I
   HincII     MslI   MspA1I     NspI     PpiI     PsrI    PvuII     SapI
     SfcI    SnaBI     SpeI     SphI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI