Restriction map for
pF1KE0395
(Linear) (Six-base) MAPSORT of: KIEE0395.seq Check: 6904 from: 1 to: 3564
KIEE0395.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2672 3564
Size: 2672 892
AccI GT'mk_AC
Cuts at: 0 1535 3564
Size: 1535 2029
Acc65I G'GTAC_C
Cuts at: 0 557 3564
Size: 557 3007
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 2425 3564
Size: 451 1974 1139
Fragments arranged by size:
1974 1139 451
AfeI AGC'GCT
Cuts at: 0 1167 3564
Size: 1167 2397
AflIII A'CryG_T
Cuts at: 0 1964 2579 3564
Size: 1964 615 985
Fragments arranged by size:
1964 985 615
AhdI GACnn_n'nnGTC
Cuts at: 0 2676 3564
Size: 2676 888
AleI CACnn'nnGTG
Cuts at: 0 18 3564
Size: 18 3546
AlwNI CAG_nnn'CTG
Cuts at: 0 666 3354 3417 3564
Size: 666 2688 63 147
Fragments arranged by size:
2688 666 147 63
ApaI G_GGCC'C
Cuts at: 0 1613 1994 2454 2792 3564
Size: 1613 381 460 338 772
Fragments arranged by size:
1613 772 460 381 338
ApaLI G'TGCA_C
Cuts at: 0 3544 3564
Size: 3544 20
ApoI r'AATT_y
Cuts at: 0 101 1504 2414 2952 3386 3405 3564
Size: 101 1403 910 538 434 19 159
Fragments arranged by size:
1403 910 538 434 159 101 19
AseI AT'TA_AT
Cuts at: 0 1201 3564
Size: 1201 2363
AvaI C'yCGr_G
Cuts at: 0 3083 3264 3564
Size: 3083 181 300
Fragments arranged by size:
3083 300 181
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1295 1328 3564
Size: 1295 33 2236
Fragments arranged by size:
2236 1295 33
BamHI G'GATC_C
Cuts at: 0 147 3564
Size: 147 3417
BanI G'GyrC_C
Cuts at: 0 557 3564
Size: 557 3007
BanII G_rGCy'C
Cuts at: 0 8 170 1613 1756 1994 2454 2792 3007
Size: 8 162 1443 143 238 460 338 215
Cuts at: 3007 3564
Size: 557
Fragments arranged by size:
1443 557 460 338 238 215 162 143
8
BbsI GAAGACnn'nnnn_
Cuts at: 0 245 326 3564
Size: 245 81 3238
Fragments arranged by size:
3238 245 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 712 746 3564
Size: 712 34 2818
Fragments arranged by size:
2818 712 34
BclI T'GATC_A
Cuts at: 0 2147 2706 3564
Size: 2147 559 858
Fragments arranged by size:
2147 858 559
BglII A'GATC_T
Cuts at: 0 2718 3564
Size: 2718 846
Bme1580I G_kGCm'C
Cuts at: 0 1613 1707 1994 2454 2792 3548 3564
Size: 1613 94 287 460 338 756 16
Fragments arranged by size:
1613 756 460 338 287 94 16
BmgBI CAC'GTC
Cuts at: 0 2262 2654 3564
Size: 2262 392 910
Fragments arranged by size:
2262 910 392
BmrI ACTGGGnnnn_n'
Cuts at: 0 540 1093 1997 2808 3545 3564
Size: 540 553 904 811 737 19
Fragments arranged by size:
904 811 737 553 540 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 27 3153 3564
Size: 27 3126 411
Fragments arranged by size:
3126 411 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 154 2097 2968 3067 3261 3564
Size: 1 153 1943 871 99 194 303
Fragments arranged by size:
1943 871 303 194 153 99 1
Bpu10I CC'TnA_GC
Cuts at: 0 1454 3564
Size: 1454 2110
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 1909 3494 3564
Size: 466 1443 1585 70
Fragments arranged by size:
1585 1443 466 70
BsaI GGTCTCn'nnnn_
Cuts at: 0 3123 3564
Size: 3123 441
BsaWI w'CCGG_w
Cuts at: 0 2993 3564
Size: 2993 571
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1607 1637 3243 3273 3564
Size: 1607 30 1606 30 291
Fragments arranged by size:
1607 1606 291 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1606 1625 3210 3564
Size: 1606 19 1585 354
Fragments arranged by size:
1606 1585 354 19
BseYI C'CCAG_C
Cuts at: 0 1464 1786 1896 3289 3530 3564
Size: 1464 322 110 1393 241 34
Fragments arranged by size:
1464 1393 322 241 110 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 969 1860 2644 2974 3564
Size: 969 891 784 330 590
Fragments arranged by size:
969 891 784 590 330
BsiEI CG_ry'CG
Cuts at: 0 3031 3564
Size: 3031 533
BsiHKAI G_wGCw'C
Cuts at: 0 170 1162 1635 2537 3548 3564
Size: 170 992 473 902 1011 16
Fragments arranged by size:
1011 992 902 473 170 16
BsmI GAATG_Cn'
Cuts at: 0 3180 3564
Size: 3180 384
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1822 3564
Size: 1822 1742
Bsp1286I G_dGCh'C
Cuts at: 0 8 170 1162 1613 1635 1707 1756 1994
Size: 8 162 992 451 22 72 49 238
Cuts at: 1994 2454 2537 2792 3007 3548 3564
Size: 460 83 255 215 541 16
Fragments arranged by size:
992 541 460 451 255 238 215 162
83 72 49 22 16 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2672 3564
Size: 2672 892
BsrBI CCG'CTC
Cuts at: 0 563 3564
Size: 563 3001
BsrDI GCAATG_nn'
Cuts at: 0 284 635 1865 3564
Size: 284 351 1230 1699
Fragments arranged by size:
1699 1230 351 284
BsrFI r'CCGG_y
Cuts at: 0 197 554 2240 2752 3564
Size: 197 357 1686 512 812
Fragments arranged by size:
1686 812 512 357 197
BsrGI T'GTAC_A
Cuts at: 0 2582 3419 3564
Size: 2582 837 145
Fragments arranged by size:
2582 837 145
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2098 3564
Size: 2098 1466
BstYI r'GATC_y
Cuts at: 0 147 662 2343 2718 3564
Size: 147 515 1681 375 846
Fragments arranged by size:
1681 846 515 375 147
BtgI C'CryG_G
Cuts at: 0 267 1853 2007 2208 3564
Size: 267 1586 154 201 1356
Fragments arranged by size:
1586 1356 267 201 154
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 737 3564
Size: 737 2827
BtsI GCAGTG_nn'
Cuts at: 0 647 1908 3095 3564
Size: 647 1261 1187 469
Fragments arranged by size:
1261 1187 647 469
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2126 2161 3564
Size: 2126 35 1403
Fragments arranged by size:
2126 1403 35
DraI TTT'AAA
Cuts at: 0 1041 3564
Size: 1041 2523
DraIII CAC_nnn'GTG
Cuts at: 0 37 2940 3564
Size: 37 2903 624
Fragments arranged by size:
2903 624 37
DrdI GACnn_nn'nnGTC
Cuts at: 0 2398 3564
Size: 2398 1166
EaeI y'GGCC_r
Cuts at: 0 2069 2211 2242 3028 3564
Size: 2069 142 31 786 536
Fragments arranged by size:
2069 786 536 142 31
EagI C'GGCC_G
Cuts at: 0 3028 3564
Size: 3028 536
EarI CTCTTCn'nnn_
Cuts at: 0 179 777 938 2285 3564
Size: 179 598 161 1347 1279
Fragments arranged by size:
1347 1279 598 179 161
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3206 3564
Size: 3206 358
EcoICRI GAG'CTC
Cuts at: 0 168 3564
Size: 168 3396
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 154 451 2097 2425 2968 3067 3261
Size: 1 153 297 1646 328 543 99 194
Cuts at: 3261 3564
Size: 303
Fragments arranged by size:
1646 543 328 303 297 194 153 99
1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 402 502 3564
Size: 402 100 3062
Fragments arranged by size:
3062 402 100
EcoO109I rG'GnC_Cy
Cuts at: 0 1609 1941 2450 2451 2760 2788 2789 2890
Size: 1609 332 509 1 309 28 1 101
Cuts at: 2890 3465 3564
Size: 575 99
Fragments arranged by size:
1609 575 509 332 309 101 99 28
1 1
HaeII r_GCGC'y
Cuts at: 0 1169 3564
Size: 1169 2395
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 847 879 1387 1419 2479 2511
Size: 577 32 238 32 508 32 1060 32
Cuts at: 2511 2662 2694 3393 3425 3564
Size: 151 32 699 32 139
Fragments arranged by size:
1060 699 577 508 238 151 139 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 446 3564
Size: 446 3118
HindIII A'AGCT_T
Cuts at: 0 313 2080 3208 3564
Size: 313 1767 1128 356
Fragments arranged by size:
1767 1128 356 313
KpnI G_GTAC'C
Cuts at: 0 561 3564
Size: 561 3003
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 1595 3564
Size: 836 759 1969
Fragments arranged by size:
1969 836 759
MscI TGG'CCA
Cuts at: 0 2071 2213 3564
Size: 2071 142 1351
Fragments arranged by size:
2071 1351 142
MslI CAynn'nnrTG
Cuts at: 0 18 640 1130 2881 3564
Size: 18 622 490 1751 683
Fragments arranged by size:
1751 683 622 490 18
MspA1I CmG'CkG
Cuts at: 0 174 1812 1896 2354 3417 3534 3564
Size: 174 1638 84 458 1063 117 30
Fragments arranged by size:
1638 1063 458 174 117 84 30
NaeI GCC'GGC
Cuts at: 0 199 2242 3564
Size: 199 2043 1322
Fragments arranged by size:
2043 1322 199
NcoI C'CATG_G
Cuts at: 0 267 1853 2007 2208 3564
Size: 267 1586 154 201 1356
Fragments arranged by size:
1586 1356 267 201 154
NdeI CA'TA_TG
Cuts at: 0 1212 2228 3459 3564
Size: 1212 1016 1231 105
Fragments arranged by size:
1231 1212 1016 105
NgoMIV G'CCGG_C
Cuts at: 0 197 2240 3564
Size: 197 2043 1324
Fragments arranged by size:
2043 1324 197
NspI r_CATG'y
Cuts at: 0 1968 2472 2583 3564
Size: 1968 504 111 981
Fragments arranged by size:
1968 981 504 111
PasI CC'CwG_GG
Cuts at: 0 249 627 1669 3564
Size: 249 378 1042 1895
Fragments arranged by size:
1895 1042 378 249
PciI A'CATG_T
Cuts at: 0 1964 2579 3564
Size: 1964 615 985
Fragments arranged by size:
1964 985 615
PfoI T'CCnGG_A
Cuts at: 0 581 3247 3564
Size: 581 2666 317
Fragments arranged by size:
2666 581 317
PpuMI rG'GwC_Cy
Cuts at: 0 1941 2760 3465 3564
Size: 1941 819 705 99
Fragments arranged by size:
1941 819 705 99
PsiI TTA'TAA
Cuts at: 0 986 3564
Size: 986 2578
PspOMI G'GGCC_C
Cuts at: 0 1609 1990 2450 2788 3564
Size: 1609 381 460 338 776
Fragments arranged by size:
1609 776 460 381 338
PstI C_TGCA'G
Cuts at: 0 2359 3311 3564
Size: 2359 952 253
Fragments arranged by size:
2359 952 253
PvuII CAG'CTG
Cuts at: 0 174 1896 3417 3534 3564
Size: 174 1722 1521 117 30
Fragments arranged by size:
1722 1521 174 117 30
SacI G_AGCT'C
Cuts at: 0 170 3564
Size: 170 3394
ScaI AGT'ACT
Cuts at: 0 550 3313 3564
Size: 550 2763 251
Fragments arranged by size:
2763 550 251
SexAI A'CCwGG_T
Cuts at: 0 323 3564
Size: 323 3241
SfcI C'TryA_G
Cuts at: 0 2355 2520 2714 3307 3446 3564
Size: 2355 165 194 593 139 118
Fragments arranged by size:
2355 593 194 165 139 118
SmaI CCC'GGG
Cuts at: 0 3085 3564
Size: 3085 479
SmlI C'TyrA_G
Cuts at: 0 481 1924 3509 3564
Size: 481 1443 1585 55
Fragments arranged by size:
1585 1443 481 55
SrfI GCCC'GGGC
Cuts at: 0 3085 3564
Size: 3085 479
StuI AGG'CCT
Cuts at: 0 397 3136 3564
Size: 397 2739 428
Fragments arranged by size:
2739 428 397
StyI C'CwwG_G
Cuts at: 0 111 267 398 842 1563 1661 1853 2007
Size: 111 156 131 444 721 98 192 154
Cuts at: 2007 2208 3110 3564
Size: 201 902 454
Fragments arranged by size:
902 721 454 444 201 192 156 154
131 111 98
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2889 2996 3564
Size: 2889 107 568
Fragments arranged by size:
2889 568 107
TatI w'GTAC_w
Cuts at: 0 434 548 668 2582 3311 3419 3553 3564
Size: 434 114 120 1914 729 108 134 11
Fragments arranged by size:
1914 729 434 134 120 114 108 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1607 1639 2167 2199 3564
Size: 1607 32 528 32 1365
Fragments arranged by size:
1607 1365 528 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 325 2883 3564
Size: 325 2558 681
Fragments arranged by size:
2558 681 325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2206 3564
Size: 2206 1358
XmaI C'CCGG_G
Cuts at: 0 3083 3564
Size: 3083 481
XmnI GAAnn'nnTTC
Cuts at: 0 786 3564
Size: 786 2778
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BamHI
BanI BanII BbsI BcgI BclI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII HindIII KpnI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NspI PasI PciI PfoI PpuMI
PsiI PspOMI PstI PvuII SacI ScaI SexAI SfcI
SmaI SmlI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AlfI AloI AscI AsiSI
AvrII BbeI BbvCI BciVI BfrBI BglI BlpI BmtI
BsaAI BsaBI BsaHI BsiWI BspEI BspHI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I ClaI EcoRI EcoRV
FalI FseI FspI FspAI HpaI KasI MfeI MluI
NarI NheI NotI NruI NsiI PacI PflMI PmeI
PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SspI SwaI XbaI XhoI ZraI