Restriction map for
pF1KE0393
(Linear) (Six-base) MAPSORT of: KIEE0393.seq Check: 9232 from: 1 to: 2865
KIEE0393.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 646 2263 2865
Size: 646 1617 602
Fragments arranged by size:
1617 646 602
AatII G_ACGT'C
Cuts at: 0 2726 2865
Size: 2726 139
AccI GT'mk_AC
Cuts at: 0 62 140 2009 2865
Size: 62 78 1869 856
Fragments arranged by size:
1869 856 78 62
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 622 745 872 1078 1203 2752 2865
Size: 616 6 123 127 206 125 1549 113
Fragments arranged by size:
1549 616 206 127 125 123 113 6
AflIII A'CryG_T
Cuts at: 0 659 2408 2516 2865
Size: 659 1749 108 349
Fragments arranged by size:
1749 659 349 108
AhdI GACnn_n'nnGTC
Cuts at: 0 58 2865
Size: 58 2807
AleI CACnn'nnGTG
Cuts at: 0 1220 2823 2865
Size: 1220 1603 42
Fragments arranged by size:
1603 1220 42
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 498 530 2865
Size: 498 32 2335
Fragments arranged by size:
2335 498 32
AlwNI CAG_nnn'CTG
Cuts at: 0 384 786 1056 1653 1743 2109 2298 2865
Size: 384 402 270 597 90 366 189 567
Fragments arranged by size:
597 567 402 384 366 270 189 90
ApaI G_GGCC'C
Cuts at: 0 2660 2834 2865
Size: 2660 174 31
Fragments arranged by size:
2660 174 31
ApaLI G'TGCA_C
Cuts at: 0 406 2865
Size: 406 2459
AvaI C'yCGr_G
Cuts at: 0 899 1458 2432 2691 2865
Size: 899 559 974 259 174
Fragments arranged by size:
974 899 559 259 174
BamHI G'GATC_C
Cuts at: 0 2763 2865
Size: 2763 102
BanI G'GyrC_C
Cuts at: 0 191 345 711 871 1231 1263 1779 2389
Size: 191 154 366 160 360 32 516 610
Cuts at: 2389 2865
Size: 476
Fragments arranged by size:
610 516 476 366 360 191 160 154
32
BanII G_rGCy'C
Cuts at: 0 566 1205 1628 1867 2000 2225 2577 2660
Size: 566 639 423 239 133 225 352 83
Cuts at: 2660 2802 2834 2865
Size: 142 32 31
Fragments arranged by size:
639 566 423 352 239 225 142 133
83 32 31
BbeI G_GCGC'C
Cuts at: 0 1783 2865
Size: 1783 1082
BbsI GAAGACnn'nnnn_
Cuts at: 0 457 964 2865
Size: 457 507 1901
Fragments arranged by size:
1901 507 457
BbvCI CC'TCA_GC
Cuts at: 0 877 1279 2865
Size: 877 402 1586
Fragments arranged by size:
1586 877 402
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 274 308 2865
Size: 274 34 2557
Fragments arranged by size:
2557 274 34
BclI T'GATC_A
Cuts at: 0 1019 2865
Size: 1019 1846
BglI GCCn_nnn'nGGC
Cuts at: 0 1461 1777 2865
Size: 1461 316 1088
Fragments arranged by size:
1461 1088 316
BglII A'GATC_T
Cuts at: 0 2276 2865
Size: 2276 589
Bme1580I G_kGCm'C
Cuts at: 0 194 410 1088 1266 1274 1334 2141 2642
Size: 194 216 678 178 8 60 807 501
Cuts at: 2642 2660 2834 2865
Size: 18 174 31
Fragments arranged by size:
807 678 501 216 194 178 174 60
31 18 8
BmgBI CAC'GTC
Cuts at: 0 375 1017 1641 2865
Size: 375 642 624 1224
Fragments arranged by size:
1224 642 624 375
BmrI ACTGGGnnnn_n'
Cuts at: 0 1622 2645 2865
Size: 1622 1023 220
Fragments arranged by size:
1622 1023 220
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1492 1522 2131 2865
Size: 53 1439 30 609 734
Fragments arranged by size:
1439 734 609 53 30
Bpu10I CC'TnA_GC
Cuts at: 0 877 1172 1279 2865
Size: 877 295 107 1586
Fragments arranged by size:
1586 877 295 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 577 1893 2600 2865
Size: 577 1316 707 265
Fragments arranged by size:
1316 707 577 265
BsaAI yAC'GTr
Cuts at: 0 1074 1974 2865
Size: 1074 900 891
Fragments arranged by size:
1074 900 891
BsaHI Gr'CG_yC
Cuts at: 0 1780 1808 2723 2865
Size: 1780 28 915 142
Fragments arranged by size:
1780 915 142 28
BsaWI w'CCGG_w
Cuts at: 0 1482 2865
Size: 1482 1383
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2182 2212 2485 2515 2521 2551 2865
Size: 2182 30 273 30 6 30 314
Fragments arranged by size:
2182 314 273 30 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1290 1489 2865
Size: 1290 199 1376
Fragments arranged by size:
1376 1290 199
BseYI C'CCAG_C
Cuts at: 0 812 1044 1653 1700 2439 2865
Size: 812 232 609 47 739 426
Fragments arranged by size:
812 739 609 426 232 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 799 906 913 1198 1430 1612 1624
Size: 397 402 107 7 285 232 182 12
Cuts at: 1624 1863 2865
Size: 239 1002
Fragments arranged by size:
1002 402 397 285 239 232 182 107
12 7
BsiEI CG_ry'CG
Cuts at: 0 312 1411 2865
Size: 312 1099 1454
Fragments arranged by size:
1454 1099 312
BsiHKAI G_wGCw'C
Cuts at: 0 410 858 1205 1421 1670 2021 2865
Size: 410 448 347 216 249 351 844
Fragments arranged by size:
844 448 410 351 347 249 216
BsmI GAATG_Cn'
Cuts at: 0 2334 2865
Size: 2334 531
BsmBI CGTCTCn'nnnn_
Cuts at: 0 265 1854 2865
Size: 265 1589 1011
Fragments arranged by size:
1589 1011 265
Bsp1286I G_dGCh'C
Cuts at: 0 194 410 566 858 1088 1205 1266 1274
Size: 194 216 156 292 230 117 61 8
Cuts at: 1274 1334 1421 1628 1670 1867 2000 2021 2141
Size: 60 87 207 42 197 133 21 120
Cuts at: 2141 2225 2577 2642 2660 2802 2834 2865
Size: 84 352 65 18 142 32 31
Fragments arranged by size:
352 292 230 216 207 197 194 156
142 133 120 117 87 84 65 61
60 42 32 31 21 18 8
BspEI T'CCGG_A
Cuts at: 0 1482 2865
Size: 1482 1383
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 19 217 617 646 977 1220 2263 2865
Size: 19 198 400 29 331 243 1043 602
Fragments arranged by size:
1043 602 400 331 243 198 29 19
BsrBI CCG'CTC
Cuts at: 0 1526 2865
Size: 1526 1339
BsrDI GCAATG_nn'
Cuts at: 0 1170 1390 2622 2865
Size: 1170 220 1232 243
Fragments arranged by size:
1232 1170 243 220
BsrFI r'CCGG_y
Cuts at: 0 348 1128 1658 2865
Size: 348 780 530 1207
Fragments arranged by size:
1207 780 530 348
BstAPI GCAn_nnn'nTGC
Cuts at: 0 87 786 1743 2865
Size: 87 699 957 1122
Fragments arranged by size:
1122 957 699 87
BstEII G'GTnAC_C
Cuts at: 0 6 1098 1155 1350 2865
Size: 6 1092 57 195 1515
Fragments arranged by size:
1515 1092 195 57 6
BstXI CCAn_nnnn'nTGG
Cuts at: 0 369 544 835 939 1421 1860 2775 2825
Size: 369 175 291 104 482 439 915 50
Cuts at: 2825 2865
Size: 40
Fragments arranged by size:
915 482 439 369 291 175 104 50
40
BstYI r'GATC_y
Cuts at: 0 2276 2763 2865
Size: 2276 487 102
Fragments arranged by size:
2276 487 102
Bsu36I CC'TnA_GG
Cuts at: 0 2396 2865
Size: 2396 469
BtgI C'CryG_G
Cuts at: 0 707 828 1346 1405 2834 2865
Size: 707 121 518 59 1429 31
Fragments arranged by size:
1429 707 518 121 59 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 437 1692 2865
Size: 437 1255 1173
Fragments arranged by size:
1255 1173 437
BtsI GCAGTG_nn'
Cuts at: 0 42 181 787 826 841 1088 1447 1987
Size: 42 139 606 39 15 247 359 540
Cuts at: 1987 2799 2865
Size: 812 66
Fragments arranged by size:
812 606 540 359 247 139 66 42
39 15
DrdI GACnn_nn'nnGTC
Cuts at: 0 1282 2865
Size: 1282 1583
EaeI y'GGCC_r
Cuts at: 0 309 350 1408 2837 2865
Size: 309 41 1058 1429 28
Fragments arranged by size:
1429 1058 309 41 28
EagI C'GGCC_G
Cuts at: 0 309 1408 2865
Size: 309 1099 1457
Fragments arranged by size:
1457 1099 309
EarI CTCTTCn'nnn_
Cuts at: 0 1885 2865
Size: 1885 980
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1881 2029 2146 2865
Size: 1881 148 117 719
Fragments arranged by size:
1881 719 148 117
EcoICRI GAG'CTC
Cuts at: 0 1203 2865
Size: 1203 1662
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 616 622 745 872 1078 1203 1492
Size: 53 563 6 123 127 206 125 289
Cuts at: 1492 1522 2131 2752 2865
Size: 30 609 621 113
Fragments arranged by size:
621 609 563 289 206 127 125 123
113 53 30 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1882 2865
Size: 1882 983
EcoO109I rG'GnC_Cy
Cuts at: 0 591 1053 1529 2295 2657 2830 2865
Size: 591 462 476 766 362 173 35
Fragments arranged by size:
766 591 476 462 362 173 35
FseI GG_CCGG'CC
Cuts at: 0 1662 2865
Size: 1662 1203
FspI TGC'GCA
Cuts at: 0 280 2865
Size: 280 2585
HaeII r_GCGC'y
Cuts at: 0 31 236 1654 1783 2486 2865
Size: 31 205 1418 129 703 379
Fragments arranged by size:
1418 703 379 205 129 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 44 76 511 543 1849 1881 2021 2053
Size: 44 32 435 32 1306 32 140 32
Cuts at: 2053 2182 2214 2865
Size: 129 32 651
Fragments arranged by size:
1306 651 435 140 129 44 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 477 552 966 1293 2010 2865
Size: 477 75 414 327 717 855
Fragments arranged by size:
855 717 477 414 327 75
KasI G'GCGC_C
Cuts at: 0 1779 2865
Size: 1779 1086
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 1877 2865
Size: 506 1371 988
Fragments arranged by size:
1371 988 506
MscI TGG'CCA
Cuts at: 0 2839 2865
Size: 2839 26
MslI CAynn'nnrTG
Cuts at: 0 166 367 1076 1220 1327 2147 2823 2865
Size: 166 201 709 144 107 820 676 42
Fragments arranged by size:
820 709 676 201 166 144 107 42
MspA1I CmG'CkG
Cuts at: 0 816 1044 1407 1538 1596 1740 1829 2106
Size: 816 228 363 131 58 144 89 277
Cuts at: 2106 2823 2865
Size: 717 42
Fragments arranged by size:
816 717 363 277 228 144 131 89
58 42
NaeI GCC'GGC
Cuts at: 0 350 1130 1660 2865
Size: 350 780 530 1205
Fragments arranged by size:
1205 780 530 350
NarI GG'CG_CC
Cuts at: 0 1780 2865
Size: 1780 1085
NcoI C'CATG_G
Cuts at: 0 828 2834 2865
Size: 828 2006 31
Fragments arranged by size:
2006 828 31
NgoMIV G'CCGG_C
Cuts at: 0 348 1128 1658 2865
Size: 348 780 530 1207
Fragments arranged by size:
1207 780 530 348
NotI GC'GGCC_GC
Cuts at: 0 309 1408 2865
Size: 309 1099 1457
Fragments arranged by size:
1457 1099 309
NruI TCG'CGA
Cuts at: 0 1820 2865
Size: 1820 1045
NspI r_CATG'y
Cuts at: 0 94 107 1140 2412 2520 2865
Size: 94 13 1033 1272 108 345
Fragments arranged by size:
1272 1033 345 108 94 13
PasI CC'CwG_GG
Cuts at: 0 760 849 864 1258 2865
Size: 760 89 15 394 1607
Fragments arranged by size:
1607 760 394 89 15
PciI A'CATG_T
Cuts at: 0 2408 2516 2865
Size: 2408 108 349
Fragments arranged by size:
2408 349 108
PflMI CCAn_nnn'nTGG
Cuts at: 0 1653 2865
Size: 1653 1212
PfoI T'CCnGG_A
Cuts at: 0 149 248 2560 2865
Size: 149 99 2312 305
Fragments arranged by size:
2312 305 149 99
PmlI CAC'GTG
Cuts at: 0 1074 2865
Size: 1074 1791
PpuMI rG'GwC_Cy
Cuts at: 0 591 1053 1529 2295 2865
Size: 591 462 476 766 570
Fragments arranged by size:
766 591 570 476 462
PshAI GACnn'nnGTC
Cuts at: 0 589 2865
Size: 589 2276
PspOMI G'GGCC_C
Cuts at: 0 2656 2830 2865
Size: 2656 174 35
Fragments arranged by size:
2656 174 35
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1954 1986 2865
Size: 1954 32 879
Fragments arranged by size:
1954 879 32
PstI C_TGCA'G
Cuts at: 0 176 821 1982 2865
Size: 176 645 1161 883
Fragments arranged by size:
1161 883 645 176
PvuII CAG'CTG
Cuts at: 0 816 1044 1596 1740 2106 2823 2865
Size: 816 228 552 144 366 717 42
Fragments arranged by size:
816 717 552 366 228 144 42
RsrII CG'GwC_CG
Cuts at: 0 2082 2865
Size: 2082 783
SacI G_AGCT'C
Cuts at: 0 1205 2865
Size: 1205 1660
SacII CC_GC'GG
Cuts at: 0 1408 2865
Size: 1408 1457
SalI G'TCGA_C
Cuts at: 0 2008 2865
Size: 2008 857
SfcI C'TryA_G
Cuts at: 0 172 817 1902 1978 2025 2865
Size: 172 645 1085 76 47 840
Fragments arranged by size:
1085 840 645 172 76 47
SfoI GGC'GCC
Cuts at: 0 1781 2865
Size: 1781 1084
SmaI CCC'GGG
Cuts at: 0 901 2693 2865
Size: 901 1792 172
Fragments arranged by size:
1792 901 172
SphI G_CATG'C
Cuts at: 0 94 2865
Size: 94 2771
SrfI GCCC'GGGC
Cuts at: 0 2693 2865
Size: 2693 172
StuI AGG'CCT
Cuts at: 0 2430 2865
Size: 2430 435
StyI C'CwwG_G
Cuts at: 0 587 828 1334 2834 2865
Size: 587 241 506 1500 31
Fragments arranged by size:
1500 587 506 241 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 841 2140 2353 2865
Size: 841 1299 213 512
Fragments arranged by size:
1299 841 512 213
TatI w'GTAC_w
Cuts at: 0 469 2114 2865
Size: 469 1645 751
Fragments arranged by size:
1645 751 469
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1939 1971 2521 2553 2865
Size: 1939 32 550 32 312
Fragments arranged by size:
1939 550 312 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2005 2865
Size: 2005 860
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 936 1149 2775 2847 2865
Size: 936 213 1626 72 18
Fragments arranged by size:
1626 936 213 72 18
XmaI C'CCGG_G
Cuts at: 0 899 2691 2865
Size: 899 1792 174
Fragments arranged by size:
1792 899 174
ZraI GAC'GTC
Cuts at: 0 2724 2865
Size: 2724 141
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AloI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BclI BglI BglII Bme1580I BmgBI
BmrI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI FspI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NruI NspI PasI
PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PsrI
PstI PvuII RsrII SacI SacII SalI SfcI SfoI
SmaI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AlfI ApoI AscI
AseI AsiSI AvrII BaeI BciVI BfrBI BlpI BmtI
BplI BpuEI BsaBI BsiWI BspHI BsrGI BssHII BssSI
BstBI BstZ17I ClaI CspCI DraI DraIII EcoRI EcoRV
FalI FspAI HindIII HpaI KpnI MfeI MluI NdeI
NheI NsiI PacI PmeI PpiI PsiI PspXI PvuI
SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SspI SwaI XbaI XhoI XmnI