Restriction map for
pF1KE0391
(Linear) (Six-base) MAPSORT of: KIEE0391.seq Check: 9934 from: 1 to: 3591
KIEE0391.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 607 3538 3570 3591
Size: 607 2931 32 21
Fragments arranged by size:
2931 607 32 21
AclI AA'CG_TT
Cuts at: 0 1395 3591
Size: 1395 2196
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 818 1679 2179 2632 3070 3289 3591
Size: 166 652 861 500 453 438 219 302
Fragments arranged by size:
861 652 500 453 438 302 219 166
AfeI AGC'GCT
Cuts at: 0 857 3591
Size: 857 2734
AflII C'TTAA_G
Cuts at: 0 766 3310 3591
Size: 766 2544 281
Fragments arranged by size:
2544 766 281
AflIII A'CryG_T
Cuts at: 0 338 1142 3591
Size: 338 804 2449
Fragments arranged by size:
2449 804 338
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 829 976 3591
Size: 829 147 2615
Fragments arranged by size:
2615 829 147
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1359 1391 3591
Size: 1359 32 2200
Fragments arranged by size:
2200 1359 32
AlwNI CAG_nnn'CTG
Cuts at: 0 654 3591
Size: 654 2937
ApaI G_GGCC'C
Cuts at: 0 682 3591
Size: 682 2909
ApoI r'AATT_y
Cuts at: 0 90 394 747 1153 1580 1682 1945 2578
Size: 90 304 353 406 427 102 263 633
Cuts at: 2578 2680 2755 2793 2857 3287 3361 3508 3591
Size: 102 75 38 64 430 74 147 83
Fragments arranged by size:
633 430 427 406 353 304 263 147
102 102 90 83 75 74 64 38
AseI AT'TA_AT
Cuts at: 0 773 3591
Size: 773 2818
AvaI C'yCGr_G
Cuts at: 0 2399 2804 3591
Size: 2399 405 787
Fragments arranged by size:
2399 787 405
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 548 581 3591
Size: 548 33 3010
Fragments arranged by size:
3010 548 33
BamHI G'GATC_C
Cuts at: 0 500 3591
Size: 500 3091
BanI G'GyrC_C
Cuts at: 0 2222 2432 3591
Size: 2222 210 1159
Fragments arranged by size:
2222 1159 210
BanII G_rGCy'C
Cuts at: 0 682 2811 3591
Size: 682 2129 780
Fragments arranged by size:
2129 780 682
BbsI GAAGACnn'nnnn_
Cuts at: 0 389 1045 2146 3524 3591
Size: 389 656 1101 1378 67
Fragments arranged by size:
1378 1101 656 389 67
BbvCI CC'TCA_GC
Cuts at: 0 2099 3591
Size: 2099 1492
BclI T'GATC_A
Cuts at: 0 3207 3591
Size: 3207 384
BfrBI ATG'CAT
Cuts at: 0 3471 3591
Size: 3471 120
BglII A'GATC_T
Cuts at: 0 1748 3591
Size: 1748 1843
BlpI GC'TnA_GC
Cuts at: 0 2439 3591
Size: 2439 1152
Bme1580I G_kGCm'C
Cuts at: 0 682 872 3591
Size: 682 190 2719
Fragments arranged by size:
2719 682 190
BmgBI CAC'GTC
Cuts at: 0 879 1134 3591
Size: 879 255 2457
Fragments arranged by size:
2457 879 255
BmtI G_CTAG'C
Cuts at: 0 2839 3591
Size: 2839 752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2191 2257 2318 3591
Size: 2191 66 61 1273
Fragments arranged by size:
2191 1273 66 61
Bpu10I CC'TnA_GC
Cuts at: 0 2099 2515 3591
Size: 2099 416 1076
Fragments arranged by size:
2099 1076 416
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 912 1606 3187 3591
Size: 912 694 1581 404
Fragments arranged by size:
1581 912 694 404
BsaAI yAC'GTr
Cuts at: 0 1145 1507 3591
Size: 1145 362 2084
Fragments arranged by size:
2084 1145 362
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1213 1243 3591
Size: 1213 30 2348
Fragments arranged by size:
2348 1213 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 98 1234 2681 3308 3591
Size: 98 1136 1447 627 283
Fragments arranged by size:
1447 1136 627 283 98
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 276 789 1498 3392 3591
Size: 276 513 709 1894 199
Fragments arranged by size:
1894 709 513 276 199
BsiHKAI G_wGCw'C
Cuts at: 0 2158 3591
Size: 2158 1433
BsmI GAATG_Cn'
Cuts at: 0 1038 2816 3473 3591
Size: 1038 1778 657 118
Fragments arranged by size:
1778 1038 657 118
Bsp1286I G_dGCh'C
Cuts at: 0 682 872 2158 2811 3591
Size: 682 190 1286 653 780
Fragments arranged by size:
1286 780 682 653 190
BspHI T'CATG_A
Cuts at: 0 2636 3591
Size: 2636 955
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1725 3030 3591
Size: 1725 1305 561
Fragments arranged by size:
1725 1305 561
BsrDI GCAATG_nn'
Cuts at: 0 149 3591
Size: 149 3442
BsrGI T'GTAC_A
Cuts at: 0 3371 3591
Size: 3371 220
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2239 2461 3591
Size: 2239 222 1130
Fragments arranged by size:
2239 1130 222
BstEII G'GTnAC_C
Cuts at: 0 3017 3591
Size: 3017 574
BstYI r'GATC_y
Cuts at: 0 249 500 1748 2379 3522 3591
Size: 249 251 1248 631 1143 69
Fragments arranged by size:
1248 1143 631 251 249 69
BstZ17I GTA'TAC
Cuts at: 0 608 3539 3591
Size: 608 2931 52
Fragments arranged by size:
2931 608 52
BtgI C'CryG_G
Cuts at: 0 872 3591
Size: 872 2719
BtsI GCAGTG_nn'
Cuts at: 0 251 3077 3591
Size: 251 2826 514
Fragments arranged by size:
2826 514 251
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3053 3088 3591
Size: 3053 35 503
Fragments arranged by size:
3053 503 35
DraI TTT'AAA
Cuts at: 0 2624 3591
Size: 2624 967
EaeI y'GGCC_r
Cuts at: 0 1854 2936 2969 3591
Size: 1854 1082 33 622
Fragments arranged by size:
1854 1082 622 33
EarI CTCTTCn'nnn_
Cuts at: 0 837 1450 1991 2778 3032 3137 3591
Size: 837 613 541 787 254 105 454
Fragments arranged by size:
837 787 613 541 454 254 105
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 818 1679 2179 2191 2257 2318 2632
Size: 166 652 861 500 12 66 61 314
Cuts at: 2632 3070 3289 3591
Size: 438 219 302
Fragments arranged by size:
861 652 500 438 314 302 219 166
66 61 12
EcoO109I rG'GnC_Cy
Cuts at: 0 678 3003 3591
Size: 678 2325 588
Fragments arranged by size:
2325 678 588
EcoRI G'AATT_C
Cuts at: 0 394 2578 3591
Size: 394 2184 1013
Fragments arranged by size:
2184 1013 394
EcoRV GAT'ATC
Cuts at: 0 1676 3591
Size: 1676 1915
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1100 3591
Size: 1100 2491
HaeII r_GCGC'y
Cuts at: 0 648 859 3591
Size: 648 211 2732
Fragments arranged by size:
2732 648 211
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 37 63 69 1213 1226 1245 1258
Size: 31 6 26 6 1144 13 19 13
Cuts at: 1258 1459 1491 2133 2165 3199 3231 3514 3546
Size: 201 32 642 32 1034 32 283 32
Cuts at: 3546 3591
Size: 45
Fragments arranged by size:
1144 1034 642 283 201 45 32 32
32 32 31 26 19 13 13 6
6
HincII GTy'rAC
Cuts at: 0 2801 3480 3591
Size: 2801 679 111
Fragments arranged by size:
2801 679 111
HpaI GTT'AAC
Cuts at: 0 2801 3480 3591
Size: 2801 679 111
Fragments arranged by size:
2801 679 111
MfeI C'AATT_G
Cuts at: 0 1805 1850 2852 3591
Size: 1805 45 1002 739
Fragments arranged by size:
1805 1002 739 45
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 969 3591
Size: 969 2622
MscI TGG'CCA
Cuts at: 0 1856 2938 2971 3591
Size: 1856 1082 33 620
Fragments arranged by size:
1856 1082 620 33
MslI CAynn'nnrTG
Cuts at: 0 161 3591
Size: 161 3430
MspA1I CmG'CkG
Cuts at: 0 2161 2199 3591
Size: 2161 38 1392
Fragments arranged by size:
2161 1392 38
NheI G'CTAG_C
Cuts at: 0 2835 3591
Size: 2835 756
NsiI A_TGCA'T
Cuts at: 0 3473 3591
Size: 3473 118
NspI r_CATG'y
Cuts at: 0 342 1704 2073 3591
Size: 342 1362 369 1518
Fragments arranged by size:
1518 1362 369 342
PciI A'CATG_T
Cuts at: 0 338 3591
Size: 338 3253
PfoI T'CCnGG_A
Cuts at: 0 2273 2334 3591
Size: 2273 61 1257
Fragments arranged by size:
2273 1257 61
PmlI CAC'GTG
Cuts at: 0 1145 3591
Size: 1145 2446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 131 163 3591
Size: 131 32 3428
Fragments arranged by size:
3428 131 32
PpuMI rG'GwC_Cy
Cuts at: 0 3003 3591
Size: 3003 588
PsiI TTA'TAA
Cuts at: 0 2033 3591
Size: 2033 1558
PspOMI G'GGCC_C
Cuts at: 0 678 3591
Size: 678 2913
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 212 244 3116 3148 3591
Size: 212 32 2872 32 443
Fragments arranged by size:
2872 443 212 32 32
PstI C_TGCA'G
Cuts at: 0 136 1722 1839 2208 2219 2573 3591
Size: 136 1586 117 369 11 354 1018
Fragments arranged by size:
1586 1018 369 354 136 117 11
PvuII CAG'CTG
Cuts at: 0 2199 3591
Size: 2199 1392
SapI GCTCTTCn'nnn_
Cuts at: 0 3032 3591
Size: 3032 559
SbfI CC_TGCA'GG
Cuts at: 0 1722 3591
Size: 1722 1869
SfcI C'TryA_G
Cuts at: 0 132 1212 1718 1835 2204 2215 2569 3057
Size: 132 1080 506 117 369 11 354 488
Cuts at: 3057 3591
Size: 534
Fragments arranged by size:
1080 534 506 488 369 354 132 117
11
SmlI C'TyrA_G
Cuts at: 0 766 891 1621 3166 3310 3591
Size: 766 125 730 1545 144 281
Fragments arranged by size:
1545 766 730 281 144 125
SpeI A'CTAG_T
Cuts at: 0 561 3591
Size: 561 3030
SphI G_CATG'C
Cuts at: 0 2073 3591
Size: 2073 1518
SspI AAT'ATT
Cuts at: 0 423 1567 2407 3591
Size: 423 1144 840 1184
Fragments arranged by size:
1184 1144 840 423
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 95 3591
Size: 95 3496
TatI w'GTAC_w
Cuts at: 0 2304 3371 3591
Size: 2304 1067 220
Fragments arranged by size:
2304 1067 220
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2307 2339 2951 2983 3591
Size: 2307 32 612 32 608
Fragments arranged by size:
2307 612 608 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2145 3591
Size: 2145 1446
XbaI T'CTAG_A
Cuts at: 0 1605 3591
Size: 1605 1986
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1687 3591
Size: 1687 1904
XmnI GAAnn'nnTTC
Cuts at: 0 3333 3591
Size: 3333 258
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AflIII AlfI AloI
AlwNI ApaI ApoI AseI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BclI BfrBI BglII BlpI Bme1580I
BmgBI BmtI BpmI Bpu10I BpuEI BsaAI BsaXI BseRI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrGI
BstAPI BstEII BstYI BstZ17I BtgI BtsI CspCI DraI
EaeI EarI Eco57MI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HpaI MfeI MmeI MscI MslI MspA1I
NheI NsiI NspI PciI PfoI PmlI PpiI PpuMI
PsiI PspOMI PsrI PstI PvuII SapI SbfI SfcI
SmlI SpeI SphI SspI TaqII TatI TstI Tth111I
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AgeI AhdI AleI ApaLI AscI
AsiSI AvrII BbeI BcgI BciVI BglI BmrI BplI
BsaI BsaBI BsaHI BsaWI BseYI BsiEI BsiWI BsmBI
BspEI BsrBI BsrFI BssHII BssSI BstBI BstXI Bsu36I
BtgZI ClaI DraIII DrdI EagI EciI EcoICRI EcoNI
FseI FspI FspAI HindIII KasI KpnI MluI NaeI
NarI NcoI NdeI NgoMIV NotI NruI PacI PasI
PflMI PmeI PshAI PspXI PvuI RsrII SacI SacII
SalI SanDI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SrfI StuI StyI SwaI XhoI XmaI
ZraI