Restriction map for
pF1KE0389
(Linear) (Six-base) MAPSORT of: KIEE0389.seq Check: 3049 from: 1 to: 2508
KIEE0389.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 785 1322 2508
Size: 785 537 1186
Fragments arranged by size:
1186 785 537
AccI GT'mk_AC
Cuts at: 0 1670 2508
Size: 1670 838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 609 1422 1839 2508
Size: 316 293 813 417 669
Fragments arranged by size:
813 669 417 316 293
AflIII A'CryG_T
Cuts at: 0 1213 2508
Size: 1213 1295
AgeI A'CCGG_T
Cuts at: 0 1259 2508
Size: 1259 1249
AhdI GACnn_n'nnGTC
Cuts at: 0 297 2508
Size: 297 2211
AleI CACnn'nnGTG
Cuts at: 0 1218 1717 2508
Size: 1218 499 791
Fragments arranged by size:
1218 791 499
AlwNI CAG_nnn'CTG
Cuts at: 0 1398 1476 1596 1907 2508
Size: 1398 78 120 311 601
Fragments arranged by size:
1398 601 311 120 78
ApaI G_GGCC'C
Cuts at: 0 2104 2349 2508
Size: 2104 245 159
Fragments arranged by size:
2104 245 159
AscI GG'CGCG_CC
Cuts at: 0 61 2508
Size: 61 2447
AvaI C'yCGr_G
Cuts at: 0 855 874 1497 1838 2050 2508
Size: 855 19 623 341 212 458
Fragments arranged by size:
855 623 458 341 212 19
BamHI G'GATC_C
Cuts at: 0 971 2508
Size: 971 1537
BanI G'GyrC_C
Cuts at: 0 1293 1353 1383 2299 2508
Size: 1293 60 30 916 209
Fragments arranged by size:
1293 916 209 60 30
BanII G_rGCy'C
Cuts at: 0 746 862 2057 2104 2349 2492 2508
Size: 746 116 1195 47 245 143 16
Fragments arranged by size:
1195 746 245 143 116 47 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 2095 2508
Size: 2095 413
BbvCI CC'TCA_GC
Cuts at: 0 1844 2508
Size: 1844 664
BclI T'GATC_A
Cuts at: 0 1817 2508
Size: 1817 691
BglI GCCn_nnn'nGGC
Cuts at: 0 1502 2508
Size: 1502 1006
BglII A'GATC_T
Cuts at: 0 1850 2508
Size: 1850 658
BlpI GC'TnA_GC
Cuts at: 0 693 1369 2508
Size: 693 676 1139
Fragments arranged by size:
1139 693 676
Bme1580I G_kGCm'C
Cuts at: 0 1298 1358 1742 2104 2302 2349 2508
Size: 1298 60 384 362 198 47 159
Fragments arranged by size:
1298 384 362 198 159 60 47
BmgBI CAC'GTC
Cuts at: 0 752 1216 2508
Size: 752 464 1292
Fragments arranged by size:
1292 752 464
BmrI ACTGGGnnnn_n'
Cuts at: 0 1292 1698 2508
Size: 1292 406 810
Fragments arranged by size:
1292 810 406
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 910 1273 1569 2325 2508
Size: 236 674 363 296 756 183
Fragments arranged by size:
756 674 363 296 236 183
Bpu10I CC'TnA_GC
Cuts at: 0 1844 2508
Size: 1844 664
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1065 2508
Size: 1065 1443
BsaI GGTCTCn'nnnn_
Cuts at: 0 27 1739 2508
Size: 27 1712 769
Fragments arranged by size:
1712 769 27
BsaAI yAC'GTr
Cuts at: 0 1035 2508
Size: 1035 1473
BsaWI w'CCGG_w
Cuts at: 0 1259 2508
Size: 1259 1249
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1174 1204 1471 1501 2015 2045 2173 2203
Size: 1174 30 267 30 514 30 128 30
Cuts at: 2203 2407 2437 2508
Size: 204 30 71
Fragments arranged by size:
1174 514 267 204 128 71 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 847 1861 2372 2386 2508
Size: 847 1014 511 14 122
Fragments arranged by size:
1014 847 511 122 14
BseYI C'CCAG_C
Cuts at: 0 51 135 1465 1564 2508
Size: 51 84 1330 99 944
Fragments arranged by size:
1330 944 99 84 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1233 2508
Size: 755 478 1275
Fragments arranged by size:
1275 755 478
BsiEI CG_ry'CG
Cuts at: 0 495 2290 2508
Size: 495 1795 218
Fragments arranged by size:
1795 495 218
BsiHKAI G_wGCw'C
Cuts at: 0 746 1877 2508
Size: 746 1131 631
Fragments arranged by size:
1131 746 631
BsmI GAATG_Cn'
Cuts at: 0 1810 2508
Size: 1810 698
BsmBI CGTCTCn'nnnn_
Cuts at: 0 795 2508
Size: 795 1713
Bsp1286I G_dGCh'C
Cuts at: 0 746 862 1298 1358 1742 1877 2057 2104
Size: 746 116 436 60 384 135 180 47
Cuts at: 2104 2302 2349 2492 2508
Size: 198 47 143 16
Fragments arranged by size:
746 436 384 198 180 143 135 116
60 47 47 16
BspHI T'CATG_A
Cuts at: 0 746 1814 2508
Size: 746 1068 694
Fragments arranged by size:
1068 746 694
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 785 1153 1322 1964 2508
Size: 785 368 169 642 544
Fragments arranged by size:
785 642 544 368 169
BsrBI CCG'CTC
Cuts at: 0 641 837 1779 1784 1940 2508
Size: 641 196 942 5 156 568
Fragments arranged by size:
942 641 568 196 156 5
BsrDI GCAATG_nn'
Cuts at: 0 543 629 2508
Size: 543 86 1879
Fragments arranged by size:
1879 543 86
BsrFI r'CCGG_y
Cuts at: 0 4 1259 2285 2508
Size: 4 1255 1026 223
Fragments arranged by size:
1255 1026 223 4
BssHII G'CGCG_C
Cuts at: 0 61 1806 2508
Size: 61 1745 702
Fragments arranged by size:
1745 702 61
BssSI C'ACGA_G
Cuts at: 0 104 1414 1447 1935 2508
Size: 104 1310 33 488 573
Fragments arranged by size:
1310 573 488 104 33
BstEII G'GTnAC_C
Cuts at: 0 1542 1644 2508
Size: 1542 102 864
Fragments arranged by size:
1542 864 102
BstYI r'GATC_y
Cuts at: 0 275 971 1517 1850 2508
Size: 275 696 546 333 658
Fragments arranged by size:
696 658 546 333 275
Bsu36I CC'TnA_GG
Cuts at: 0 436 734 1675 2508
Size: 436 298 941 833
Fragments arranged by size:
941 833 436 298
BtgI C'CryG_G
Cuts at: 0 509 595 1081 2219 2303 2492 2508
Size: 509 86 486 1138 84 189 16
Fragments arranged by size:
1138 509 486 189 86 84 16
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1995 2508
Size: 1995 513
BtsI GCAGTG_nn'
Cuts at: 0 31 2508
Size: 31 2477
ClaI AT'CG_AT
Cuts at: 0 500 2508
Size: 500 2008
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1970 2005 2508
Size: 1970 35 503
Fragments arranged by size:
1970 503 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1999 2508
Size: 1999 509
EaeI y'GGCC_r
Cuts at: 0 2 492 1451 2287 2363 2508
Size: 2 490 959 836 76 145
Fragments arranged by size:
959 836 490 145 76 2
EagI C'GGCC_G
Cuts at: 0 492 2287 2508
Size: 492 1795 221
Fragments arranged by size:
1795 492 221
EarI CTCTTCn'nnn_
Cuts at: 0 1966 2508
Size: 1966 542
EcoICRI GAG'CTC
Cuts at: 0 744 2508
Size: 744 1764
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 316 609 910 1273 1422 1569 1839
Size: 236 80 293 301 363 149 147 270
Cuts at: 1839 2325 2508
Size: 486 183
Fragments arranged by size:
486 363 301 293 270 236 183 149
147 80
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1864 2508
Size: 1864 644
EcoO109I rG'GnC_Cy
Cuts at: 0 140 1473 1679 1698 2100 2101 2345 2346
Size: 140 1333 206 19 402 1 244 1
Cuts at: 2346 2508
Size: 162
Fragments arranged by size:
1333 402 244 206 162 140 19 1
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1905 2508
Size: 1905 603
FseI GG_CCGG'CC
Cuts at: 0 8 2508
Size: 8 2500
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 132 164 283 315 1493 1525 2015 2047
Size: 132 32 119 32 1178 32 490 32
Cuts at: 2047 2173 2205 2508
Size: 126 32 303
Fragments arranged by size:
1178 490 303 132 126 119 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1041 1914 2508
Size: 1041 873 594
Fragments arranged by size:
1041 873 594
HindIII A'AGCT_T
Cuts at: 0 83 1887 2508
Size: 83 1804 621
Fragments arranged by size:
1804 621 83
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 839 1026 1596 2508
Size: 839 187 570 912
Fragments arranged by size:
912 839 570 187
MscI TGG'CCA
Cuts at: 0 1453 2365 2508
Size: 1453 912 143
Fragments arranged by size:
1453 912 143
MslI CAynn'nnrTG
Cuts at: 0 1218 1540 1717 1735 2198 2508
Size: 1218 322 177 18 463 310
Fragments arranged by size:
1218 463 322 310 177 18
MspA1I CmG'CkG
Cuts at: 0 491 1833 2017 2107 2252 2508
Size: 491 1342 184 90 145 256
Fragments arranged by size:
1342 491 256 184 145 90
NaeI GCC'GGC
Cuts at: 0 6 2508
Size: 6 2502
NcoI C'CATG_G
Cuts at: 0 595 2219 2303 2492 2508
Size: 595 1624 84 189 16
Fragments arranged by size:
1624 595 189 84 16
NdeI CA'TA_TG
Cuts at: 0 2335 2508
Size: 2335 173
NgoMIV G'CCGG_C
Cuts at: 0 4 2508
Size: 4 2504
PflMI CCAn_nnn'nTGG
Cuts at: 0 996 2508
Size: 996 1512
PfoI T'CCnGG_A
Cuts at: 0 550 1339 1723 1823 2508
Size: 550 789 384 100 685
Fragments arranged by size:
789 685 550 384 100
PpuMI rG'GwC_Cy
Cuts at: 0 140 1473 1679 2508
Size: 140 1333 206 829
Fragments arranged by size:
1333 829 206 140
PspOMI G'GGCC_C
Cuts at: 0 2100 2345 2508
Size: 2100 245 163
Fragments arranged by size:
2100 245 163
PstI C_TGCA'G
Cuts at: 0 419 535 986 1150 1403 1930 2267 2508
Size: 419 116 451 164 253 527 337 241
Fragments arranged by size:
527 451 419 337 253 241 164 116
RsrII CG'GwC_CG
Cuts at: 0 448 2508
Size: 448 2060
SacI G_AGCT'C
Cuts at: 0 746 2508
Size: 746 1762
SanDI GG'GwC_CC
Cuts at: 0 140 2508
Size: 140 2368
SapI GCTCTTCn'nnn_
Cuts at: 0 1966 2508
Size: 1966 542
SexAI A'CCwGG_T
Cuts at: 0 1748 2508
Size: 1748 760
SfcI C'TryA_G
Cuts at: 0 415 531 982 1146 1399 1926 2239 2263
Size: 415 116 451 164 253 527 313 24
Cuts at: 2263 2508
Size: 245
Fragments arranged by size:
527 451 415 313 253 245 164 116
24
SmaI CCC'GGG
Cuts at: 0 857 1499 1840 2508
Size: 857 642 341 668
Fragments arranged by size:
857 668 642 341
SmlI C'TyrA_G
Cuts at: 0 1044 2508
Size: 1044 1464
SspI AAT'ATT
Cuts at: 0 288 2508
Size: 288 2220
StuI AGG'CCT
Cuts at: 0 1582 2508
Size: 1582 926
StyI C'CwwG_G
Cuts at: 0 595 1358 1981 2219 2303 2492 2508
Size: 595 763 623 238 84 189 16
Fragments arranged by size:
763 623 595 238 189 84 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 43 436 2343 2508
Size: 43 393 1907 165
Fragments arranged by size:
1907 393 165 43
TatI w'GTAC_w
Cuts at: 0 1478 2508
Size: 1478 1030
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 2451 2483 2508
Size: 1174 32 1245 32 25
Fragments arranged by size:
1245 1174 32 32 25
Tth111I GACn'n_nGTC
Cuts at: 0 472 1602 2508
Size: 472 1130 906
Fragments arranged by size:
1130 906 472
XbaI T'CTAG_A
Cuts at: 0 1131 2508
Size: 1131 1377
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1749 2434 2508
Size: 1749 685 74
Fragments arranged by size:
1749 685 74
XmaI C'CCGG_G
Cuts at: 0 855 1497 1838 2508
Size: 855 642 341 670
Fragments arranged by size:
855 670 642 341
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AhdI AleI AlwNI
ApaI AscI AvaI BamHI BanI BanII BbsI BbvCI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DrdI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI Hin4I
HincII HindIII MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV PflMI PfoI PpuMI PspOMI PstI RsrII
SacI SanDI SapI SexAI SfcI SmaI SmlI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AlfI AloI ApaLI
ApoI AseI AsiSI AvrII BaeI BbeI BcgI BciVI
BfrBI BmtI BplI BsaBI BsaHI BsiWI BspEI BsrGI
BstAPI BstBI BstXI BstZ17I DraI DraIII EciI EcoRI
EcoRV FspI FspAI HaeII HpaI KasI KpnI MfeI
MluI NarI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI PvuII SacII SalI SbfI ScaI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SwaI
XhoI XmnI ZraI