Restriction map for
pF1KE0384
(Linear) (Six-base) MAPSORT of: KIEE0384.seq Check: 3042 from: 1 to: 1467
KIEE0384.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 0 785 1467
Size: 785 682
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1108 1467
Size: 1108 359
AflIII A'CryG_T
Cuts at: 0 878 1467
Size: 878 589
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 955 1467
Size: 955 512
AlwNI CAG_nnn'CTG
Cuts at: 0 640 693 705 1341 1467
Size: 640 53 12 636 126
Fragments arranged by size:
640 636 126 53 12
ApaI G_GGCC'C
Cuts at: 0 130 179 233 288 488 515 1467
Size: 130 49 54 55 200 27 952
Fragments arranged by size:
952 200 130 55 54 49 27
ApaLI G'TGCA_C
Cuts at: 0 252 1467
Size: 252 1215
AvaI C'yCGr_G
Cuts at: 0 105 123 190 226 907 994 1467
Size: 105 18 67 36 681 87 473
Fragments arranged by size:
681 473 105 87 67 36 18
BamHI G'GATC_C
Cuts at: 0 1220 1467
Size: 1220 247
BanI G'GyrC_C
Cuts at: 0 16 82 109 195 478 958 970 1103
Size: 16 66 27 86 283 480 12 133
Cuts at: 1103 1199 1467
Size: 96 268
Fragments arranged by size:
480 283 268 133 96 86 66 27
16 12
BanII G_rGCy'C
Cuts at: 0 130 179 233 288 428 488 515 914
Size: 130 49 54 55 140 60 27 399
Cuts at: 914 1040 1177 1467
Size: 126 137 290
Fragments arranged by size:
399 290 140 137 130 126 60 55
54 49 27
BbeI G_GCGC'C
Cuts at: 0 86 113 482 1467
Size: 86 27 369 985
Fragments arranged by size:
985 369 86 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 1400 1467
Size: 1400 67
BbvCI CC'TCA_GC
Cuts at: 0 733 822 1467
Size: 733 89 645
Fragments arranged by size:
733 645 89
BciVI GTATCCnnnnn_n'
Cuts at: 0 1239 1467
Size: 1239 228
BclI T'GATC_A
Cuts at: 0 1052 1467
Size: 1052 415
BglI GCCn_nnn'nGGC
Cuts at: 0 61 228 476 1276 1467
Size: 61 167 248 800 191
Fragments arranged by size:
800 248 191 167 61
BlpI GC'TnA_GC
Cuts at: 0 803 1467
Size: 803 664
Bme1580I G_kGCm'C
Cuts at: 0 47 130 179 198 233 256 288 488
Size: 47 83 49 19 35 23 32 200
Cuts at: 488 515 650 973 995 1467
Size: 27 135 323 22 472
Fragments arranged by size:
472 323 200 135 83 49 47 35
32 27 23 22 19
BmrI ACTGGGnnnn_n'
Cuts at: 0 628 1293 1467
Size: 628 665 174
Fragments arranged by size:
665 628 174
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 559 565 607 700 733 821 829
Size: 457 102 6 42 93 33 88 8
Cuts at: 829 1467
Size: 638
Fragments arranged by size:
638 457 102 93 88 42 33 8
6
Bpu10I CC'TnA_GC
Cuts at: 0 142 733 822 1081 1467
Size: 142 591 89 259 386
Fragments arranged by size:
591 386 259 142 89
BsaBI GATnn'nnATC
Cuts at: 0 856 1225 1467
Size: 856 369 242
Fragments arranged by size:
856 369 242
BsaHI Gr'CG_yC
Cuts at: 0 83 110 479 551 782 1467
Size: 83 27 369 72 231 685
Fragments arranged by size:
685 369 231 83 72 27
BsaWI w'CCGG_w
Cuts at: 0 1177 1467
Size: 1177 290
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 802 832 1467
Size: 802 30 635
Fragments arranged by size:
802 635 30
BseYI C'CCAG_C
Cuts at: 0 287 438 627 1070 1371 1467
Size: 287 151 189 443 301 96
Fragments arranged by size:
443 301 287 189 151 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 1341 1467
Size: 136 1205 126
Fragments arranged by size:
1205 136 126
BsiEI CG_ry'CG
Cuts at: 0 101 356 582 1467
Size: 101 255 226 885
Fragments arranged by size:
885 255 226 101
BsiHKAI G_wGCw'C
Cuts at: 0 256 352 428 815 914 1467
Size: 256 96 76 387 99 553
Fragments arranged by size:
553 387 256 99 96 76
BsmI GAATG_Cn'
Cuts at: 0 919 1467
Size: 919 548
Bsp1286I G_dGCh'C
Cuts at: 0 47 130 179 198 233 256 288 352
Size: 47 83 49 19 35 23 32 64
Cuts at: 352 428 488 515 650 815 914 973 995
Size: 76 60 27 135 165 99 59 22
Cuts at: 995 1040 1177 1467
Size: 45 137 290
Fragments arranged by size:
290 165 137 135 99 83 76 64
60 59 49 47 45 35 32 27
23 22 19
BsrBI CCG'CTC
Cuts at: 0 455 1467
Size: 455 1012
BsrDI GCAATG_nn'
Cuts at: 0 1292 1346 1467
Size: 1292 54 121
Fragments arranged by size:
1292 121 54
BsrFI r'CCGG_y
Cuts at: 0 430 652 749 961 1259 1467
Size: 430 222 97 212 298 208
Fragments arranged by size:
430 298 222 212 208 97
BssHII G'CGCG_C
Cuts at: 0 146 209 391 558 1467
Size: 146 63 182 167 909
Fragments arranged by size:
909 182 167 146 63
BstAPI GCAn_nnn'nTGC
Cuts at: 0 219 1467
Size: 219 1248
BstYI r'GATC_y
Cuts at: 0 1220 1467
Size: 1220 247
BtgI C'CryG_G
Cuts at: 0 200 500 1040 1467
Size: 200 300 540 427
Fragments arranged by size:
540 427 300 200
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 18 1101 1467
Size: 18 1083 366
Fragments arranged by size:
1083 366 18
BtsI GCAGTG_nn'
Cuts at: 0 1358 1366 1467
Size: 1358 8 101
Fragments arranged by size:
1358 101 8
EaeI y'GGCC_r
Cuts at: 0 53 203 353 503 963 1257 1467
Size: 53 150 150 150 460 294 210
Fragments arranged by size:
460 294 210 150 150 150 53
EagI C'GGCC_G
Cuts at: 0 353 1467
Size: 353 1114
EarI CTCTTCn'nnn_
Cuts at: 0 323 1467
Size: 323 1144
EcoICRI GAG'CTC
Cuts at: 0 426 912 1467
Size: 426 486 555
Fragments arranged by size:
555 486 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 559 565 607 700 733 821 829
Size: 457 102 6 42 93 33 88 8
Cuts at: 829 1108 1467
Size: 279 359
Fragments arranged by size:
457 359 279 102 93 88 42 33
8 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 589 1467
Size: 589 878
EcoO109I rG'GnC_Cy
Cuts at: 0 176 230 284 485 512 818 1338 1467
Size: 176 54 54 201 27 306 520 129
Fragments arranged by size:
520 306 201 176 129 54 54 27
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1452 1467
Size: 1452 15
HaeII r_GCGC'y
Cuts at: 0 73 86 113 311 379 482 1467
Size: 73 13 27 198 68 103 985
Fragments arranged by size:
985 198 103 73 68 27 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 802 834 1467
Size: 802 32 633
Fragments arranged by size:
802 633 32
KasI G'GCGC_C
Cuts at: 0 82 109 478 1467
Size: 82 27 369 989
Fragments arranged by size:
989 369 82 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 758 1467
Size: 758 709
MspA1I CmG'CkG
Cuts at: 0 135 202 219 261 363 446 622 678
Size: 135 67 17 42 102 83 176 56
Cuts at: 678 690 956 1074 1467
Size: 12 266 118 393
Fragments arranged by size:
393 266 176 135 118 102 83 67
56 42 17 12
NaeI GCC'GGC
Cuts at: 0 751 1467
Size: 751 716
NarI GG'CG_CC
Cuts at: 0 83 110 479 1467
Size: 83 27 369 988
Fragments arranged by size:
988 369 83 27
NcoI C'CATG_G
Cuts at: 0 500 1040 1467
Size: 500 540 427
Fragments arranged by size:
540 500 427
NgoMIV G'CCGG_C
Cuts at: 0 749 1467
Size: 749 718
NspI r_CATG'y
Cuts at: 0 882 936 1467
Size: 882 54 531
Fragments arranged by size:
882 531 54
PasI CC'CwG_GG
Cuts at: 0 515 1251 1388 1467
Size: 515 736 137 79
Fragments arranged by size:
736 515 137 79
PciI A'CATG_T
Cuts at: 0 878 1467
Size: 878 589
PflMI CCAn_nnn'nTGG
Cuts at: 0 705 1341 1467
Size: 705 636 126
Fragments arranged by size:
705 636 126
PpuMI rG'GwC_Cy
Cuts at: 0 818 1338 1467
Size: 818 520 129
Fragments arranged by size:
818 520 129
PspOMI G'GGCC_C
Cuts at: 0 126 175 229 284 484 511 1467
Size: 126 49 54 55 200 27 956
Fragments arranged by size:
956 200 126 55 54 49 27
PstI C_TGCA'G
Cuts at: 0 347 383 791 1172 1467
Size: 347 36 408 381 295
Fragments arranged by size:
408 381 347 295 36
PvuII CAG'CTG
Cuts at: 0 363 622 678 690 1074 1467
Size: 363 259 56 12 384 393
Fragments arranged by size:
393 384 363 259 56 12
SacI G_AGCT'C
Cuts at: 0 428 914 1467
Size: 428 486 553
Fragments arranged by size:
553 486 428
SacII CC_GC'GG
Cuts at: 0 203 1467
Size: 203 1264
SapI GCTCTTCn'nnn_
Cuts at: 0 323 1467
Size: 323 1144
SbfI CC_TGCA'GG
Cuts at: 0 791 1467
Size: 791 676
SfcI C'TryA_G
Cuts at: 0 343 379 787 828 1168 1467
Size: 343 36 408 41 340 299
Fragments arranged by size:
408 343 340 299 41 36
SfoI GGC'GCC
Cuts at: 0 84 111 480 1467
Size: 84 27 369 987
Fragments arranged by size:
987 369 84 27
SmaI CCC'GGG
Cuts at: 0 125 192 228 996 1467
Size: 125 67 36 768 471
Fragments arranged by size:
768 471 125 67 36
SrfI GCCC'GGGC
Cuts at: 0 228 996 1467
Size: 228 768 471
Fragments arranged by size:
768 471 228
StuI AGG'CCT
Cuts at: 0 1279 1411 1467
Size: 1279 132 56
Fragments arranged by size:
1279 132 56
StyI C'CwwG_G
Cuts at: 0 500 1040 1460 1467
Size: 500 540 420 7
Fragments arranged by size:
540 500 420 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 87 1243 1288 1467
Size: 87 1156 45 179
Fragments arranged by size:
1156 179 87 45
TatI w'GTAC_w
Cuts at: 0 1014 1467
Size: 1014 453
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 45 1467
Size: 45 1422
XmaI C'CCGG_G
Cuts at: 0 123 190 226 994 1467
Size: 123 67 36 768 473
Fragments arranged by size:
768 473 123 67 36
ZraI GAC'GTC
Cuts at: 0 783 1467
Size: 783 684
Enzymes that do cut:
AatII AcuI AflIII AlfI AlwNI ApaI ApaLI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI
BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BsrBI BsrDI BsrFI BssHII BstAPI BstYI BtgI BtgZI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I KasI MmeI MspA1I NaeI NarI
NcoI NgoMIV NspI PasI PciI PflMI PpuMI PspOMI
PstI PvuII SacI SacII SapI SbfI SfcI SfoI
SmaI SrfI StuI StyI TaqII TatI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvrII BaeI
BcgI BfrBI BglII BmgBI BmtI BplI BpuEI BsaI
BsaAI BseRI BsiWI BsmBI BspEI BspHI BspMI BsrGI
BssSI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EciI EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI MscI
MslI NdeI NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI ScaI SexAI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI SwaI TstI Tth111I
XbaI XhoI XmnI