(Linear) (Six-base) MAPSORT of: KIEE0357.seq  Check: 3640  from: 1  to: 1803



KIEE0357.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:13  ..

Acc65I G'GTAC_C

Cuts at:      0     275    1803 
   Size:        275    1528
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     908     969     980    1223    1648    1803 
   Size:        908      61      11     243     425     155

  Fragments arranged by size: 

                 908     425     243     155      61      11
 
AflIII A'CryG_T

Cuts at:      0    1278    1803 
   Size:       1278     525
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1588    1803 
   Size:       1588     215
 
ApoI r'AATT_y

Cuts at:      0     480     489    1803 
   Size:        480       9    1314

  Fragments arranged by size: 

                1314     480       9
 
AseI AT'TA_AT

Cuts at:      0     853    1803 
   Size:        853     950
 
AvaI C'yCGr_G

Cuts at:      0     575    1803 
   Size:        575    1228
 
AvrII C'CTAG_G

Cuts at:      0    1555    1803 
   Size:       1555     248
 
BanI G'GyrC_C

Cuts at:      0     275    1803 
   Size:        275    1528
 
BglII A'GATC_T

Cuts at:      0     316    1012    1803 
   Size:        316     696     791

  Fragments arranged by size: 

                 791     696     316
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     697    1803 
   Size:        697    1106
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     598    1803 
   Size:        310     288    1205

  Fragments arranged by size: 

                1205     310     288
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     942     972    1803 
   Size:        942      30     831

  Fragments arranged by size: 

                 942     831      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     563    1803 
   Size:        563    1240
 
BspHI T'CATG_A

Cuts at:      0    1055    1803 
   Size:       1055     748
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     146    1596    1803 
   Size:        146    1450     207

  Fragments arranged by size: 

                1450     207     146
 
BsrDI GCAATG_nn'

Cuts at:      0     141    1573    1643    1803 
   Size:        141    1432      70     160

  Fragments arranged by size: 

                1432     160     141      70
 
BsrGI T'GTAC_A

Cuts at:      0    1221    1803 
   Size:       1221     582
 
BstYI r'GATC_y

Cuts at:      0     316    1012    1042    1803 
   Size:        316     696      30     761

  Fragments arranged by size: 

                 761     696     316      30
 
Bsu36I CC'TnA_GG

Cuts at:      0    1669    1803 
   Size:       1669     134
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     253     288    1155    1190    1803 
   Size:        253      35     867      35     613

  Fragments arranged by size: 

                 867     613     253      35      35
 
DraI TTT'AAA

Cuts at:      0     231    1803 
   Size:        231    1572
 
EarI CTCTTCn'nnn_

Cuts at:      0      16     418     794    1624    1803 
   Size:         16     402     376     830     179

  Fragments arranged by size: 

                 830     402     376     179      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     598     908     969     980    1223    1648    1803 
   Size:        310     288     310      61      11     243     425     155

  Fragments arranged by size: 

                 425     310     310     288     243     155      61      11
            
 
EcoRI G'AATT_C

Cuts at:      0     480    1803 
   Size:        480    1323
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     301     333     942     974    1803 
   Size:        301      32     609      32     829

  Fragments arranged by size: 

                 829     609     301      32      32
 
HincII GTy'rAC

Cuts at:      0    1050    1803 
   Size:       1050     753
 
HindIII A'AGCT_T

Cuts at:      0    1335    1803 
   Size:       1335     468
 
KpnI G_GTAC'C

Cuts at:      0     279    1803 
   Size:        279    1524
 
MfeI C'AATT_G

Cuts at:      0     521    1520    1803 
   Size:        521     999     283

  Fragments arranged by size: 

                 999     521     283
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1220    1246    1803 
   Size:       1220      26     557

  Fragments arranged by size: 

                1220     557      26
 
MspA1I CmG'CkG

Cuts at:      0     553    1803 
   Size:        553    1250
 
NspI r_CATG'y

Cuts at:      0    1222    1803 
   Size:       1222     581
 
PshAI GACnn'nnGTC

Cuts at:      0    1136    1803 
   Size:       1136     667
 
PstI C_TGCA'G

Cuts at:      0    1593    1803 
   Size:       1593     210
 
PvuII CAG'CTG

Cuts at:      0     553    1803 
   Size:        553    1250
 
ScaI AGT'ACT

Cuts at:      0    1762    1803 
   Size:       1762      41
 
SfcI C'TryA_G

Cuts at:      0    1589    1803 
   Size:       1589     214
 
StyI C'CwwG_G

Cuts at:      0      16    1555    1803 
   Size:         16    1539     248

  Fragments arranged by size: 

                1539     248      16
 
SwaI ATTT'AAAT

Cuts at:      0     231    1803 
   Size:        231    1572
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     712    1457    1803 
   Size:        712     745     346

  Fragments arranged by size: 

                 745     712     346
 
TatI w'GTAC_w

Cuts at:      0    1221    1760    1803 
   Size:       1221     539      43

  Fragments arranged by size: 

                1221     539      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     960     992    1803 
   Size:        960      32     811

  Fragments arranged by size: 

                 960     811      32
 
XbaI T'CTAG_A

Cuts at:      0     484    1803 
   Size:        484    1319
 
XmnI GAAnn'nnTTC

Cuts at:      0    1678    1803 
   Size:       1678     125
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII    AlwNI     ApoI     AseI     AvaI    AvrII
     BanI    BglII     BmrI     BpmI    BsaXI    BseRI    BspHI    BspMI
    BsrDI    BsrGI    BstYI   Bsu36I    CspCI     DraI     EarI  Eco57MI
    EcoRI    Hin4I   HincII  HindIII     KpnI     MfeI     MmeI   MspA1I
     NspI    PshAI     PstI    PvuII     ScaI     SfcI     StyI     SwaI
    TaqII     TatI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI     BaeI
    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BsrBI
    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII     HpaI     KasI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI  Tth111I     XcmI     XhoI     XmaI     ZraI