Restriction map for
pF1KE0347
(Linear) (Six-base) MAPSORT of: KIEE0347.seq Check: 5975 from: 1 to: 2559
KIEE0347.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 600 2039 2559
Size: 600 1439 520
Fragments arranged by size:
1439 600 520
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1247 1537 1832 2557 2559
Size: 1006 241 290 295 725 2
Fragments arranged by size:
1006 725 295 290 241 2
AfeI AGC'GCT
Cuts at: 0 1591 2559
Size: 1591 968
AflIII A'CryG_T
Cuts at: 0 1400 1574 2450 2559
Size: 1400 174 876 109
Fragments arranged by size:
1400 876 174 109
AleI CACnn'nnGTG
Cuts at: 0 2449 2559
Size: 2449 110
AlwNI CAG_nnn'CTG
Cuts at: 0 2484 2559
Size: 2484 75
ApaI G_GGCC'C
Cuts at: 0 1008 1921 2196 2559
Size: 1008 913 275 363
Fragments arranged by size:
1008 913 363 275
ApaLI G'TGCA_C
Cuts at: 0 2403 2437 2559
Size: 2403 34 122
Fragments arranged by size:
2403 122 34
ApoI r'AATT_y
Cuts at: 0 2020 2559
Size: 2020 539
AvaI C'yCGr_G
Cuts at: 0 371 2218 2260 2559
Size: 371 1847 42 299
Fragments arranged by size:
1847 371 299 42
BanI G'GyrC_C
Cuts at: 0 1610 2470 2559
Size: 1610 860 89
Fragments arranged by size:
1610 860 89
BanII G_rGCy'C
Cuts at: 0 1008 1361 1562 1921 2196 2414 2559
Size: 1008 353 201 359 275 218 145
Fragments arranged by size:
1008 359 353 275 218 201 145
BbeI G_GCGC'C
Cuts at: 0 1614 2559
Size: 1614 945
BbsI GAAGACnn'nnnn_
Cuts at: 0 59 1146 2559
Size: 59 1087 1413
Fragments arranged by size:
1413 1087 59
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 437 471 799 833 2394 2428 2559
Size: 437 34 328 34 1561 34 131
Fragments arranged by size:
1561 437 328 131 34 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 383 2559
Size: 383 2176
BlpI GC'TnA_GC
Cuts at: 0 190 2559
Size: 190 2369
Bme1580I G_kGCm'C
Cuts at: 0 1008 1528 1921 2196 2407 2441 2475 2559
Size: 1008 520 393 275 211 34 34 84
Fragments arranged by size:
1008 520 393 275 211 84 34 34
BmgBI CAC'GTC
Cuts at: 0 1881 2451 2559
Size: 1881 570 108
Fragments arranged by size:
1881 570 108
BmrI ACTGGGnnnn_n'
Cuts at: 0 247 847 1037 2559
Size: 247 600 190 1522
Fragments arranged by size:
1522 600 247 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 318 937 983 1531 1584 2161 2520
Size: 133 185 619 46 548 53 577 359
Cuts at: 2520 2559
Size: 39
Fragments arranged by size:
619 577 548 359 185 133 53 46
39
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1408 2559
Size: 1180 228 1151
Fragments arranged by size:
1180 1151 228
BsaI GGTCTCn'nnnn_
Cuts at: 0 819 2559
Size: 819 1740
BsaAI yAC'GTr
Cuts at: 0 1872 2559
Size: 1872 687
BsaBI GATnn'nnATC
Cuts at: 0 1238 1418 2559
Size: 1238 180 1141
Fragments arranged by size:
1238 1141 180
BsaHI Gr'CG_yC
Cuts at: 0 38 538 1601 1611 2559
Size: 38 500 1063 10 948
Fragments arranged by size:
1063 948 500 38 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 76 106 316 346 2559
Size: 76 30 210 30 2213
Fragments arranged by size:
2213 210 76 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 46 1837 2559
Size: 46 1791 722
Fragments arranged by size:
1791 722 46
BseYI C'CCAG_C
Cuts at: 0 300 484 952 2487 2559
Size: 300 184 468 1535 72
Fragments arranged by size:
1535 468 300 184 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1112 1416 2559
Size: 1112 304 1143
Fragments arranged by size:
1143 1112 304
BsiEI CG_ry'CG
Cuts at: 0 1541 2071 2559
Size: 1541 530 488
Fragments arranged by size:
1541 530 488
BsiHKAI G_wGCw'C
Cuts at: 0 2407 2414 2441 2559
Size: 2407 7 27 118
Fragments arranged by size:
2407 118 27 7
BsmBI CGTCTCn'nnnn_
Cuts at: 0 432 891 2559
Size: 432 459 1668
Fragments arranged by size:
1668 459 432
Bsp1286I G_dGCh'C
Cuts at: 0 1008 1361 1528 1562 1921 2196 2407 2414
Size: 1008 353 167 34 359 275 211 7
Cuts at: 2414 2441 2475 2559
Size: 27 34 84
Fragments arranged by size:
1008 359 353 275 211 167 84 34
34 27 7
BspHI T'CATG_A
Cuts at: 0 2375 2559
Size: 2375 184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 600 2039 2559
Size: 600 1439 520
Fragments arranged by size:
1439 600 520
BsrDI GCAATG_nn'
Cuts at: 0 1829 2559
Size: 1829 730
BsrFI r'CCGG_y
Cuts at: 0 40 377 1487 2006 2066 2559
Size: 40 337 1110 519 60 493
Fragments arranged by size:
1110 519 493 337 60 40
BssSI C'ACGA_G
Cuts at: 0 682 1476 1852 2559
Size: 682 794 376 707
Fragments arranged by size:
794 707 682 376
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1439 2559
Size: 1439 1120
BstBI TT'CG_AA
Cuts at: 0 2018 2559
Size: 2018 541
BstXI CCAn_nnnn'nTGG
Cuts at: 0 790 2559
Size: 790 1769
BstYI r'GATC_y
Cuts at: 0 2420 2559
Size: 2420 139
BtgI C'CryG_G
Cuts at: 0 410 469 722 1008 2466 2559
Size: 410 59 253 286 1458 93
Fragments arranged by size:
1458 410 286 253 93 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 230 809 1734 2559
Size: 230 579 925 825
Fragments arranged by size:
925 825 579 230
DraIII CAC_nnn'GTG
Cuts at: 0 1439 2559
Size: 1439 1120
DrdI GACnn_nn'nnGTC
Cuts at: 0 253 2559
Size: 253 2306
EaeI y'GGCC_r
Cuts at: 0 472 866 1538 2068 2559
Size: 472 394 672 530 491
Fragments arranged by size:
672 530 491 472 394
EagI C'GGCC_G
Cuts at: 0 1538 2068 2559
Size: 1538 530 491
Fragments arranged by size:
1538 530 491
EarI CTCTTCn'nnn_
Cuts at: 0 274 2017 2530 2559
Size: 274 1743 513 29
Fragments arranged by size:
1743 513 274 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1955 2559
Size: 1955 604
EcoICRI GAG'CTC
Cuts at: 0 2412 2559
Size: 2412 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 318 937 983 1006 1247 1531 1537
Size: 133 185 619 46 23 241 284 6
Cuts at: 1537 1584 1832 2161 2520 2557 2559
Size: 47 248 329 359 37 2
Fragments arranged by size:
619 359 329 284 248 241 185 133
47 46 37 23 6 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 574 1993 2559
Size: 574 1419 566
Fragments arranged by size:
1419 574 566
EcoO109I rG'GnC_Cy
Cuts at: 0 74 431 678 1038 1917 2192 2495 2559
Size: 74 357 247 360 879 275 303 64
Fragments arranged by size:
879 360 357 303 275 247 74 64
HaeII r_GCGC'y
Cuts at: 0 1194 1593 1614 2559
Size: 1194 399 21 945
Fragments arranged by size:
1194 945 399 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 2559
Size: 76 32 2451
Fragments arranged by size:
2451 76 32
HincII GTy'rAC
Cuts at: 0 69 2559
Size: 69 2490
HindIII A'AGCT_T
Cuts at: 0 1549 2559
Size: 1549 1010
KasI G'GCGC_C
Cuts at: 0 1610 2559
Size: 1610 949
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 977 1818 2480 2541 2559
Size: 119 858 841 662 61 18
Fragments arranged by size:
858 841 662 119 61 18
MscI TGG'CCA
Cuts at: 0 868 2559
Size: 868 1691
MslI CAynn'nnrTG
Cuts at: 0 23 727 2449 2559
Size: 23 704 1722 110
Fragments arranged by size:
1722 704 110 23
MspA1I CmG'CkG
Cuts at: 0 304 984 1150 1537 2559
Size: 304 680 166 387 1022
Fragments arranged by size:
1022 680 387 304 166
NaeI GCC'GGC
Cuts at: 0 42 379 2008 2559
Size: 42 337 1629 551
Fragments arranged by size:
1629 551 337 42
NarI GG'CG_CC
Cuts at: 0 1611 2559
Size: 1611 948
NcoI C'CATG_G
Cuts at: 0 469 722 2559
Size: 469 253 1837
Fragments arranged by size:
1837 469 253
NgoMIV G'CCGG_C
Cuts at: 0 40 377 2006 2559
Size: 40 337 1629 553
Fragments arranged by size:
1629 553 337 40
NruI TCG'CGA
Cuts at: 0 159 1753 2559
Size: 159 1594 806
Fragments arranged by size:
1594 806 159
NspI r_CATG'y
Cuts at: 0 774 1373 1404 1578 2555 2559
Size: 774 599 31 174 977 4
Fragments arranged by size:
977 774 599 174 31 4
PciI A'CATG_T
Cuts at: 0 1400 1574 2559
Size: 1400 174 985
Fragments arranged by size:
1400 985 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 915 2559
Size: 915 1644
PfoI T'CCnGG_A
Cuts at: 0 110 272 2138 2497 2559
Size: 110 162 1866 359 62
Fragments arranged by size:
1866 359 162 110 62
PpuMI rG'GwC_Cy
Cuts at: 0 431 678 2495 2559
Size: 431 247 1817 64
Fragments arranged by size:
1817 431 247 64
PspOMI G'GGCC_C
Cuts at: 0 1004 1917 2192 2559
Size: 1004 913 275 367
Fragments arranged by size:
1004 913 367 275
PspXI vC'TCGA_Gb
Cuts at: 0 2260 2559
Size: 2260 299
PstI C_TGCA'G
Cuts at: 0 82 822 1640 2270 2559
Size: 82 740 818 630 289
Fragments arranged by size:
818 740 630 289 82
PvuII CAG'CTG
Cuts at: 0 304 984 1150 2559
Size: 304 680 166 1409
Fragments arranged by size:
1409 680 304 166
SacI G_AGCT'C
Cuts at: 0 2414 2559
Size: 2414 145
SapI GCTCTTCn'nnn_
Cuts at: 0 274 2017 2559
Size: 274 1743 542
Fragments arranged by size:
1743 542 274
SbfI CC_TGCA'GG
Cuts at: 0 1640 2559
Size: 1640 919
SexAI A'CCwGG_T
Cuts at: 0 427 2559
Size: 427 2132
SfcI C'TryA_G
Cuts at: 0 78 818 1636 2266 2559
Size: 78 740 818 630 293
Fragments arranged by size:
818 740 630 293 78
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 383 2559
Size: 383 2176
SfoI GGC'GCC
Cuts at: 0 1612 2559
Size: 1612 947
SgrAI Cr'CCGG_yG
Cuts at: 0 40 2559
Size: 40 2519
SmaI CCC'GGG
Cuts at: 0 2220 2559
Size: 2220 339
SmlI C'TyrA_G
Cuts at: 0 1195 1387 2260 2559
Size: 1195 192 873 299
Fragments arranged by size:
1195 873 299 192
SphI G_CATG'C
Cuts at: 0 774 2559
Size: 774 1785
SspI AAT'ATT
Cuts at: 0 1905 2559
Size: 1905 654
StuI AGG'CCT
Cuts at: 0 354 1642 1988 2559
Size: 354 1288 346 571
Fragments arranged by size:
1288 571 354 346
StyI C'CwwG_G
Cuts at: 0 469 722 2051 2559
Size: 469 253 1329 508
Fragments arranged by size:
1329 508 469 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1200 1241 2559
Size: 1200 41 1318
Fragments arranged by size:
1318 1200 41
TatI w'GTAC_w
Cuts at: 0 2310 2559
Size: 2310 249
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 340 372 670 702 2559
Size: 340 32 298 32 1857
Fragments arranged by size:
1857 340 298 32 32
XhoI C'TCGA_G
Cuts at: 0 2260 2559
Size: 2260 299
XmaI C'CCGG_G
Cuts at: 0 2218 2559
Size: 2218 341
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BcgI BglI
BlpI Bme1580I BmgBI BmrI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BssSI BstAPI
BstBI BstXI BstYI BtgI BtgZI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII
Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NruI NspI PciI PflMI
PfoI PpuMI PspOMI PspXI PstI PvuII SacI SapI
SbfI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI XhoI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BfrBI BglII BmtI BplI Bpu10I BsaWI
BsiWI BsmI BspEI BsrBI BsrGI BssHII BstEII BstZ17I
Bsu36I BtsI ClaI CspCI DraI EcoRI EcoRV FalI
FseI FspI FspAI HpaI KpnI MfeI MluI NdeI
NheI NotI NsiI PacI PasI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI
ScaI SgfI SnaBI SpeI SrfI SwaI Tth111I XbaI
XcmI XmnI ZraI