Restriction map for
pF1KE0344
(Linear) (Six-base) MAPSORT of: KIEE0344.seq Check: 3311 from: 1 to: 2121
KIEE0344.orf
With 182 enzymes: SgfI *
March 2, 2010 14:31 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 665 762 2121
Size: 665 97 1359
Fragments arranged by size:
1359 665 97
AccI GT'mk_AC
Cuts at: 0 968 2121
Size: 968 1153
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1102 2121
Size: 1102 1019
AfeI AGC'GCT
Cuts at: 0 1681 2121
Size: 1681 440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 890 2121
Size: 890 1231
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1429 1461 2121
Size: 1429 32 660
Fragments arranged by size:
1429 660 32
AlwNI CAG_nnn'CTG
Cuts at: 0 168 231 569 624 777 789 870 1080
Size: 168 63 338 55 153 12 81 210
Cuts at: 1080 1962 2121
Size: 882 159
Fragments arranged by size:
882 338 210 168 159 153 81 63
55 12
ApaI G_GGCC'C
Cuts at: 0 480 1308 1543 2121
Size: 480 828 235 578
Fragments arranged by size:
828 578 480 235
ApaLI G'TGCA_C
Cuts at: 0 254 994 2121
Size: 254 740 1127
Fragments arranged by size:
1127 740 254
AvaI C'yCGr_G
Cuts at: 0 106 1276 2121
Size: 106 1170 845
Fragments arranged by size:
1170 845 106
BanI G'GyrC_C
Cuts at: 0 1254 1289 1321 1491 1730 1998 2121
Size: 1254 35 32 170 239 268 123
Fragments arranged by size:
1254 268 239 170 123 35 32
BanII G_rGCy'C
Cuts at: 0 10 276 289 480 590 1277 1308 1543
Size: 10 266 13 191 110 687 31 235
Cuts at: 1543 1677 1925 2121
Size: 134 248 196
Fragments arranged by size:
687 266 248 235 196 191 134 110
31 13 10
BbeI G_GCGC'C
Cuts at: 0 1293 1734 2121
Size: 1293 441 387
Fragments arranged by size:
1293 441 387
BbsI GAAGACnn'nnnn_
Cuts at: 0 45 497 910 1090 2121
Size: 45 452 413 180 1031
Fragments arranged by size:
1031 452 413 180 45
BbvCI CC'TCA_GC
Cuts at: 0 427 2121
Size: 427 1694
BciVI GTATCCnnnnn_n'
Cuts at: 0 1649 2121
Size: 1649 472
BglI GCCn_nnn'nGGC
Cuts at: 0 101 110 350 666 934 1939 2121
Size: 101 9 240 316 268 1005 182
Fragments arranged by size:
1005 316 268 240 182 101 9
BlpI GC'TnA_GC
Cuts at: 0 241 283 679 878 2121
Size: 241 42 396 199 1243
Fragments arranged by size:
1243 396 241 199 42
Bme1580I G_kGCm'C
Cuts at: 0 258 480 998 1149 1222 1308 1496 1543
Size: 258 222 518 151 73 86 188 47
Cuts at: 1543 2121
Size: 578
Fragments arranged by size:
578 518 258 222 188 151 86 73
47
BmrI ACTGGGnnnn_n'
Cuts at: 0 217 612 1385 2121
Size: 217 395 773 736
Fragments arranged by size:
773 736 395 217
BmtI G_CTAG'C
Cuts at: 0 1969 2121
Size: 1969 152
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 253 1026 1030 1426 1804 1918 2073
Size: 19 234 773 4 396 378 114 155
Cuts at: 2073 2121
Size: 48
Fragments arranged by size:
773 396 378 234 155 114 48 19
4
Bpu10I CC'TnA_GC
Cuts at: 0 427 1468 1795 2121
Size: 427 1041 327 326
Fragments arranged by size:
1041 427 327 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 169 607 2121
Size: 169 438 1514
Fragments arranged by size:
1514 438 169
BsaAI yAC'GTr
Cuts at: 0 1269 2121
Size: 1269 852
BsaBI GATnn'nnATC
Cuts at: 0 796 2121
Size: 796 1325
BsaHI Gr'CG_yC
Cuts at: 0 744 1290 1731 2121
Size: 744 546 441 390
Fragments arranged by size:
744 546 441 390
BsaWI w'CCGG_w
Cuts at: 0 1982 2044 2121
Size: 1982 62 77
Fragments arranged by size:
1982 77 62
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1429 1459 1681 1711 2071 2101 2121
Size: 1429 30 222 30 360 30 20
Fragments arranged by size:
1429 360 222 30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1759 1915 2121
Size: 1759 156 206
Fragments arranged by size:
1759 206 156
BseYI C'CCAG_C
Cuts at: 0 249 1110 1676 1700 2121
Size: 249 861 566 24 421
Fragments arranged by size:
861 566 421 249 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1975 2121
Size: 655 1320 146
Fragments arranged by size:
1320 655 146
BsiEI CG_ry'CG
Cuts at: 0 50 1229 1477 2121
Size: 50 1179 248 644
Fragments arranged by size:
1179 644 248 50
BsiHKAI G_wGCw'C
Cuts at: 0 258 998 1334 2121
Size: 258 740 336 787
Fragments arranged by size:
787 740 336 258
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1780 2121
Size: 1780 341
Bsp1286I G_dGCh'C
Cuts at: 0 10 258 276 289 480 590 998 1149
Size: 10 248 18 13 191 110 408 151
Cuts at: 1149 1222 1277 1308 1334 1496 1543 1677 1925
Size: 73 55 31 26 162 47 134 248
Cuts at: 1925 2121
Size: 196
Fragments arranged by size:
408 248 248 196 191 162 151 134
110 73 55 47 31 26 18 13
10
BspEI T'CCGG_A
Cuts at: 0 2044 2121
Size: 2044 77
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 665 762 774 2121
Size: 665 97 12 1347
Fragments arranged by size:
1347 665 97 12
BsrFI r'CCGG_y
Cuts at: 0 660 802 1472 2121
Size: 660 142 670 649
Fragments arranged by size:
670 660 649 142
BsrGI T'GTAC_A
Cuts at: 0 1451 2121
Size: 1451 670
BstAPI GCAn_nnn'nTGC
Cuts at: 0 777 1962 2121
Size: 777 1185 159
Fragments arranged by size:
1185 777 159
Bsu36I CC'TnA_GG
Cuts at: 0 143 543 1394 2121
Size: 143 400 851 727
Fragments arranged by size:
851 727 400 143
BtgI C'CryG_G
Cuts at: 0 19 888 1229 1284 1325 1558 1934 2121
Size: 19 869 341 55 41 233 376 187
Fragments arranged by size:
869 376 341 233 187 55 41 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 528 2121
Size: 528 1593
BtsI GCAGTG_nn'
Cuts at: 0 289 590 845 1220 2121
Size: 289 301 255 375 901
Fragments arranged by size:
901 375 301 289 255
DraIII CAC_nnn'GTG
Cuts at: 0 1106 2121
Size: 1106 1015
EaeI y'GGCC_r
Cuts at: 0 47 407 689 885 908 935 1343 1422
Size: 47 360 282 196 23 27 408 79
Cuts at: 1422 1474 1631 1931 2121
Size: 52 157 300 190
Fragments arranged by size:
408 360 300 282 196 190 157 79
52 47 27 23
EagI C'GGCC_G
Cuts at: 0 47 1474 2121
Size: 47 1427 647
Fragments arranged by size:
1427 647 47
EarI CTCTTCn'nnn_
Cuts at: 0 34 1366 2121
Size: 34 1332 755
Fragments arranged by size:
1332 755 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 517 1354 1600 2121
Size: 517 837 246 521
Fragments arranged by size:
837 521 517 246
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 19 253 1026 1030 1102 1426 1804 1918
Size: 19 234 773 4 72 324 378 114
Cuts at: 1918 2073 2121
Size: 155 48
Fragments arranged by size:
773 378 324 234 155 114 72 48
19 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1588 1858 2121
Size: 1588 270 263
Fragments arranged by size:
1588 270 263
EcoO109I rG'GnC_Cy
Cuts at: 0 228 453 476 477 786 927 1280 1304
Size: 228 225 23 1 309 141 353 24
Cuts at: 1304 1773 1863 1902 1959 2121
Size: 469 90 39 57 162
Fragments arranged by size:
469 353 309 228 225 162 141 90
57 39 24 23 1
HaeII r_GCGC'y
Cuts at: 0 159 202 1293 1683 1734 1844 2121
Size: 159 43 1091 390 51 110 277
Fragments arranged by size:
1091 390 277 159 110 51 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1866 1898 2121
Size: 1866 32 223
Fragments arranged by size:
1866 223 32
HincII GTy'rAC
Cuts at: 0 1017 2121
Size: 1017 1104
KasI G'GCGC_C
Cuts at: 0 1289 1730 2121
Size: 1289 441 391
Fragments arranged by size:
1289 441 391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 1227 2121
Size: 836 391 894
Fragments arranged by size:
894 836 391
MscI TGG'CCA
Cuts at: 0 691 887 937 1345 1633 2121
Size: 691 196 50 408 288 488
Fragments arranged by size:
691 488 408 288 196 50
MslI CAynn'nnrTG
Cuts at: 0 1933 2121
Size: 1933 188
MspA1I CmG'CkG
Cuts at: 0 21 168 426 569 1341 1887 1995 2009
Size: 21 147 258 143 772 546 108 14
Cuts at: 2009 2121
Size: 112
Fragments arranged by size:
772 546 258 147 143 112 108 21
14
NaeI GCC'GGC
Cuts at: 0 1474 2121
Size: 1474 647
NarI GG'CG_CC
Cuts at: 0 1290 1731 2121
Size: 1290 441 390
Fragments arranged by size:
1290 441 390
NgoMIV G'CCGG_C
Cuts at: 0 1472 2121
Size: 1472 649
NheI G'CTAG_C
Cuts at: 0 1965 2121
Size: 1965 156
PasI CC'CwG_GG
Cuts at: 0 225 267 459 1536 1767 2121
Size: 225 42 192 1077 231 354
Fragments arranged by size:
1077 354 231 225 192 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 231 870 917 1106 1740 2121
Size: 42 189 639 47 189 634 381
Fragments arranged by size:
639 634 381 189 189 47 42
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1429 1461 2121
Size: 1429 32 660
Fragments arranged by size:
1429 660 32
PpuMI rG'GwC_Cy
Cuts at: 0 786 1280 1773 2121
Size: 786 494 493 348
Fragments arranged by size:
786 494 493 348
PspOMI G'GGCC_C
Cuts at: 0 476 1304 1539 2121
Size: 476 828 235 582
Fragments arranged by size:
828 582 476 235
PstI C_TGCA'G
Cuts at: 0 785 1592 2121
Size: 785 807 529
Fragments arranged by size:
807 785 529
PvuII CAG'CTG
Cuts at: 0 168 1341 1887 2121
Size: 168 1173 546 234
Fragments arranged by size:
1173 546 234 168
SacII CC_GC'GG
Cuts at: 0 22 2121
Size: 22 2099
SanDI GG'GwC_CC
Cuts at: 0 1280 1773 2121
Size: 1280 493 348
Fragments arranged by size:
1280 493 348
SapI GCTCTTCn'nnn_
Cuts at: 0 34 2121
Size: 34 2087
ScaI AGT'ACT
Cuts at: 0 1690 2121
Size: 1690 431
SfcI C'TryA_G
Cuts at: 0 781 1196 1336 1588 1810 2121
Size: 781 415 140 252 222 311
Fragments arranged by size:
781 415 311 252 222 140
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 101 934 2121
Size: 101 833 1187
Fragments arranged by size:
1187 833 101
SfoI GGC'GCC
Cuts at: 0 1291 1732 2121
Size: 1291 441 389
Fragments arranged by size:
1291 441 389
SmaI CCC'GGG
Cuts at: 0 1278 2121
Size: 1278 843
SpeI A'CTAG_T
Cuts at: 0 258 2121
Size: 258 1863
StuI AGG'CCT
Cuts at: 0 95 2121
Size: 95 2026
StyI C'CwwG_G
Cuts at: 0 923 2121
Size: 923 1198
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 241 1102 1728 2121
Size: 241 861 626 393
Fragments arranged by size:
861 626 393 241
TatI w'GTAC_w
Cuts at: 0 1451 1688 2121
Size: 1451 237 433
Fragments arranged by size:
1451 433 237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1176 1208 2121
Size: 1176 32 913
Fragments arranged by size:
1176 913 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 852 930 1107 1315 1511 1851 2121
Size: 852 78 177 208 196 340 270
Fragments arranged by size:
852 340 270 208 196 177 78
XmaI C'CCGG_G
Cuts at: 0 1276 2121
Size: 1276 845
Enzymes that do cut:
AarI AccI AcuI AfeI AlfI AloI AlwNI ApaI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BciVI
BglI BlpI Bme1580I BmrI BmtI BpmI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI BsrGI
BstAPI Bsu36I BtgI BtgZI BtsI DraIII EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NheI PasI PflMI PpiI PpuMI PspOMI PstI PvuII
SacII SanDI SapI ScaI SfcI SfiI SfoI SmaI
SpeI StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BclI BfrBI BglII BmgBI BplI BpuEI BsiWI BsmI
BspHI BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI
BstYI BstZ17I ClaI CspCI DraI DrdI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NcoI NdeI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SalI SbfI SexAI SgfI
SgrAI SmlI SnaBI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmnI ZraI