Restriction map for
pF1KE0343
(Linear) (Six-base) MAPSORT of: KIEE0343.seq Check: 2267 from: 1 to: 1815
KIEE0343.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 197 233 1815
Size: 197 36 1582
Fragments arranged by size:
1582 197 36
Acc65I G'GTAC_C
Cuts at: 0 1094 1815
Size: 1094 721
AfeI AGC'GCT
Cuts at: 0 1777 1815
Size: 1777 38
AflIII A'CryG_T
Cuts at: 0 545 1185 1815
Size: 545 640 630
Fragments arranged by size:
640 630 545
AhdI GACnn_n'nnGTC
Cuts at: 0 1737 1815
Size: 1737 78
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 878 881 1815
Size: 878 3 934
Fragments arranged by size:
934 878 3
AlwNI CAG_nnn'CTG
Cuts at: 0 916 957 1248 1490 1512 1815
Size: 916 41 291 242 22 303
Fragments arranged by size:
916 303 291 242 41 22
ApaI G_GGCC'C
Cuts at: 0 909 1815
Size: 909 906
AvaI C'yCGr_G
Cuts at: 0 1339 1711 1815
Size: 1339 372 104
Fragments arranged by size:
1339 372 104
BanI G'GyrC_C
Cuts at: 0 1094 1815
Size: 1094 721
BanII G_rGCy'C
Cuts at: 0 187 697 836 853 909 1340 1430 1815
Size: 187 510 139 17 56 431 90 385
Fragments arranged by size:
510 431 385 187 139 90 56 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 1295 1815
Size: 1295 520
BclI T'GATC_A
Cuts at: 0 405 1815
Size: 405 1410
BglI GCCn_nnn'nGGC
Cuts at: 0 457 820 1815
Size: 457 363 995
Fragments arranged by size:
995 457 363
BglII A'GATC_T
Cuts at: 0 116 1815
Size: 116 1699
BlpI GC'TnA_GC
Cuts at: 0 1238 1471 1815
Size: 1238 233 344
Fragments arranged by size:
1238 344 233
Bme1580I G_kGCm'C
Cuts at: 0 909 1019 1541 1815
Size: 909 110 522 274
Fragments arranged by size:
909 522 274 110
BmgBI CAC'GTC
Cuts at: 0 9 75 1815
Size: 9 66 1740
Fragments arranged by size:
1740 66 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 72 410 1500 1815
Size: 72 338 1090 315
Fragments arranged by size:
1090 338 315 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 722 850 923 1294 1501 1738 1815
Size: 121 601 128 73 371 207 237 77
Fragments arranged by size:
601 371 237 207 128 121 77 73
Bpu10I CC'TnA_GC
Cuts at: 0 1393 1815
Size: 1393 422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 1815
Size: 382 1433
BsaI GGTCTCn'nnnn_
Cuts at: 0 1578 1815
Size: 1578 237
BsaAI yAC'GTr
Cuts at: 0 548 1188 1815
Size: 548 640 627
Fragments arranged by size:
640 627 548
BsaHI Gr'CG_yC
Cuts at: 0 412 1815
Size: 412 1403
BsaWI w'CCGG_w
Cuts at: 0 1211 1815
Size: 1211 604
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1159 1189 1815
Size: 1159 30 626
Fragments arranged by size:
1159 626 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 676 799 1045 1239 1276 1339 1417
Size: 118 558 123 246 194 37 63 78
Cuts at: 1417 1815
Size: 398
Fragments arranged by size:
558 398 246 194 123 118 78 63
37
BseYI C'CCAG_C
Cuts at: 0 83 87 702 1586 1703 1808 1815
Size: 83 4 615 884 117 105 7
Fragments arranged by size:
884 615 117 105 83 7 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 708 1525 1815
Size: 310 398 817 290
Fragments arranged by size:
817 398 310 290
BsiHKAI G_wGCw'C
Cuts at: 0 1430 1815
Size: 1430 385
Bsp1286I G_dGCh'C
Cuts at: 0 187 697 836 853 909 1019 1340 1430
Size: 187 510 139 17 56 110 321 90
Cuts at: 1430 1541 1815
Size: 111 274
Fragments arranged by size:
510 321 274 187 139 111 110 90
56 17
BspEI T'CCGG_A
Cuts at: 0 1211 1815
Size: 1211 604
BsrBI CCG'CTC
Cuts at: 0 826 897 1815
Size: 826 71 918
Fragments arranged by size:
918 826 71
BsrFI r'CCGG_y
Cuts at: 0 1632 1815
Size: 1632 183
BsrGI T'GTAC_A
Cuts at: 0 494 1815
Size: 494 1321
BssHII G'CGCG_C
Cuts at: 0 16 1815
Size: 16 1799
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1117 1248 1512 1536 1815
Size: 1117 131 264 24 279
Fragments arranged by size:
1117 279 264 131 24
BstBI TT'CG_AA
Cuts at: 0 1413 1815
Size: 1413 402
BstXI CCAn_nnnn'nTGG
Cuts at: 0 453 947 1558 1815
Size: 453 494 611 257
Fragments arranged by size:
611 494 453 257
BstYI r'GATC_y
Cuts at: 0 116 393 1815
Size: 116 277 1422
Fragments arranged by size:
1422 277 116
Bsu36I CC'TnA_GG
Cuts at: 0 803 1322 1815
Size: 803 519 493
Fragments arranged by size:
803 519 493
BtgI C'CryG_G
Cuts at: 0 446 602 1727 1802 1815
Size: 446 156 1125 75 13
Fragments arranged by size:
1125 446 156 75 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1419 1815
Size: 1419 396
BtsI GCAGTG_nn'
Cuts at: 0 1249 1385 1815
Size: 1249 136 430
Fragments arranged by size:
1249 430 136
DrdI GACnn_nn'nnGTC
Cuts at: 0 1168 1570 1815
Size: 1168 402 245
Fragments arranged by size:
1168 402 245
EaeI y'GGCC_r
Cuts at: 0 518 1626 1676 1724 1815
Size: 518 1108 50 48 91
Fragments arranged by size:
1108 518 91 50 48
EarI CTCTTCn'nnn_
Cuts at: 0 288 1435 1815
Size: 288 1147 380
Fragments arranged by size:
1147 380 288
EcoICRI GAG'CTC
Cuts at: 0 1428 1815
Size: 1428 387
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 722 850 923 1294 1501 1738 1815
Size: 121 601 128 73 371 207 237 77
Fragments arranged by size:
601 371 237 207 128 121 77 73
EcoO109I rG'GnC_Cy
Cuts at: 0 240 327 423 513 569 635 730 905
Size: 240 87 96 90 56 66 95 175
Cuts at: 905 906 1170 1815
Size: 1 264 645
Fragments arranged by size:
645 264 240 175 96 95 90 87
66 56 1
HaeII r_GCGC'y
Cuts at: 0 1067 1779 1815
Size: 1067 712 36
Fragments arranged by size:
1067 712 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 238 270 340 372 580 612 904 936
Size: 238 32 70 32 208 32 292 32
Cuts at: 936 1723 1755 1815
Size: 787 32 60
Fragments arranged by size:
787 292 238 208 70 60 32 32
32 32 32
KpnI G_GTAC'C
Cuts at: 0 1098 1815
Size: 1098 717
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 1181 1815
Size: 560 621 634
Fragments arranged by size:
634 621 560
MscI TGG'CCA
Cuts at: 0 520 1628 1678 1815
Size: 520 1108 50 137
Fragments arranged by size:
1108 520 137 50
MslI CAynn'nnrTG
Cuts at: 0 1556 1815
Size: 1556 259
MspA1I CmG'CkG
Cuts at: 0 87 613 1366 1569 1674 1815
Size: 87 526 753 203 105 141
Fragments arranged by size:
753 526 203 141 105 87
NaeI GCC'GGC
Cuts at: 0 1634 1815
Size: 1634 181
NcoI C'CATG_G
Cuts at: 0 446 602 1802 1815
Size: 446 156 1200 13
Fragments arranged by size:
1200 446 156 13
NgoMIV G'CCGG_C
Cuts at: 0 1632 1815
Size: 1632 183
NspI r_CATG'y
Cuts at: 0 597 888 1537 1815
Size: 597 291 649 278
Fragments arranged by size:
649 597 291 278
PasI CC'CwG_GG
Cuts at: 0 756 1355 1815
Size: 756 599 460
Fragments arranged by size:
756 599 460
PflMI CCAn_nnn'nTGG
Cuts at: 0 62 1815
Size: 62 1753
PfoI T'CCnGG_A
Cuts at: 0 379 844 1457 1517 1815
Size: 379 465 613 60 298
Fragments arranged by size:
613 465 379 298 60
PmlI CAC'GTG
Cuts at: 0 548 1188 1815
Size: 548 640 627
Fragments arranged by size:
640 627 548
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 1815
Size: 334 32 1449
Fragments arranged by size:
1449 334 32
PpuMI rG'GwC_Cy
Cuts at: 0 327 423 513 635 1170 1815
Size: 327 96 90 122 535 645
Fragments arranged by size:
645 535 327 122 96 90
PspOMI G'GGCC_C
Cuts at: 0 905 1815
Size: 905 910
PstI C_TGCA'G
Cuts at: 0 1079 1223 1260 1673 1815
Size: 1079 144 37 413 142
Fragments arranged by size:
1079 413 144 142 37
PvuII CAG'CTG
Cuts at: 0 87 1569 1674 1815
Size: 87 1482 105 141
Fragments arranged by size:
1482 141 105 87
SacI G_AGCT'C
Cuts at: 0 1430 1815
Size: 1430 385
SanDI GG'GwC_CC
Cuts at: 0 327 635 1815
Size: 327 308 1180
Fragments arranged by size:
1180 327 308
SapI GCTCTTCn'nnn_
Cuts at: 0 1435 1815
Size: 1435 380
SfcI C'TryA_G
Cuts at: 0 198 1075 1219 1256 1669 1815
Size: 198 877 144 37 413 146
Fragments arranged by size:
877 413 198 146 144 37
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 457 820 1815
Size: 457 363 995
Fragments arranged by size:
995 457 363
SmaI CCC'GGG
Cuts at: 0 1713 1815
Size: 1713 102
SmlI C'TyrA_G
Cuts at: 0 397 1815
Size: 397 1418
SrfI GCCC'GGGC
Cuts at: 0 1713 1815
Size: 1713 102
StuI AGG'CCT
Cuts at: 0 808 1005 1815
Size: 808 197 810
Fragments arranged by size:
810 808 197
StyI C'CwwG_G
Cuts at: 0 446 602 818 836 974 1802 1815
Size: 446 156 216 18 138 828 13
Fragments arranged by size:
828 446 216 156 138 18 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 748 1815
Size: 748 1067
TatI w'GTAC_w
Cuts at: 0 494 1175 1328 1815
Size: 494 681 153 487
Fragments arranged by size:
681 494 487 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 537 569 1815
Size: 537 32 1246
Fragments arranged by size:
1246 537 32
XbaI T'CTAG_A
Cuts at: 0 1314 1815
Size: 1314 501
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 453 825 1815
Size: 453 372 990
Fragments arranged by size:
990 453 372
XmaI C'CCGG_G
Cuts at: 0 1711 1815
Size: 1711 104
XmnI GAAnn'nnTTC
Cuts at: 0 349 532 1815
Size: 349 183 1283
Fragments arranged by size:
1283 349 183
Enzymes that do cut:
AccI Acc65I AfeI AflIII AhdI AlfI AlwNI ApaI
AvaI BanI BanII BbsI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BsrBI BsrFI BsrGI BssHII BstAPI BstBI BstXI
BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I HaeII Hin4I KpnI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PflMI
PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI
SanDI SapI SfcI SfiI SmaI SmlI SrfI StuI
StyI TaqII TatI TstI XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AcuI AflII AgeI AleI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BfrBI BmtI BplI BsaBI
BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrDI BssSI
BstEII BstZ17I ClaI CspCI DraI DraIII EagI EciI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI MfeI MluI NarI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI Tth111I XhoI ZraI