Restriction map for
pF1KE0334
(Linear) (Six-base) MAPSORT of: KIEE0334.seq Check: 2269 from: 1 to: 2625
KIEE0334.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 1574 1665 2625
Size: 644 930 91 960
Fragments arranged by size:
960 930 644 91
AfeI AGC'GCT
Cuts at: 0 704 2625
Size: 704 1921
AflIII A'CryG_T
Cuts at: 0 693 1226 2501 2625
Size: 693 533 1275 124
Fragments arranged by size:
1275 693 533 124
AleI CACnn'nnGTG
Cuts at: 0 1973 2625
Size: 1973 652
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2582 2625
Size: 2582 43
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 733 765 2625
Size: 733 32 1860
Fragments arranged by size:
1860 733 32
AlwNI CAG_nnn'CTG
Cuts at: 0 996 1473 2067 2625
Size: 996 477 594 558
Fragments arranged by size:
996 594 558 477
ApoI r'AATT_y
Cuts at: 0 313 512 1898 2200 2353 2625
Size: 313 199 1386 302 153 272
Fragments arranged by size:
1386 313 302 272 199 153
AseI AT'TA_AT
Cuts at: 0 2594 2625
Size: 2594 31
BamHI G'GATC_C
Cuts at: 0 741 1505 2625
Size: 741 764 1120
Fragments arranged by size:
1120 764 741
BanI G'GyrC_C
Cuts at: 0 1641 2625
Size: 1641 984
BanII G_rGCy'C
Cuts at: 0 716 2625
Size: 716 1909
BbsI GAAGACnn'nnnn_
Cuts at: 0 581 2625
Size: 581 2044
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 241 275 2625
Size: 241 34 2350
Fragments arranged by size:
2350 241 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 670 2625
Size: 670 1955
BclI T'GATC_A
Cuts at: 0 423 2361 2625
Size: 423 1938 264
Fragments arranged by size:
1938 423 264
BfrBI ATG'CAT
Cuts at: 0 166 733 2625
Size: 166 567 1892
Fragments arranged by size:
1892 567 166
BlpI GC'TnA_GC
Cuts at: 0 950 2625
Size: 950 1675
BmrI ACTGGGnnnn_n'
Cuts at: 0 120 2625
Size: 120 2505
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 994 1942 2625
Size: 994 948 683
Fragments arranged by size:
994 948 683
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 2625
Size: 432 2193
BsaAI yAC'GTr
Cuts at: 0 2502 2625
Size: 2502 123
BsaBI GATnn'nnATC
Cuts at: 0 1964 2246 2625
Size: 1964 282 379
Fragments arranged by size:
1964 379 282
BsaWI w'CCGG_w
Cuts at: 0 1763 2625
Size: 1763 862
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1561 1591 2625
Size: 1561 30 1034
Fragments arranged by size:
1561 1034 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1438 2210 2625
Size: 1438 772 415
Fragments arranged by size:
1438 772 415
BseYI C'CCAG_C
Cuts at: 0 16 50 2018 2625
Size: 16 34 1968 607
Fragments arranged by size:
1968 607 34 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 284 1111 1774 1931 2625
Size: 284 827 663 157 694
Fragments arranged by size:
827 694 663 284 157
BsiEI CG_ry'CG
Cuts at: 0 89 2625
Size: 89 2536
BsiHKAI G_wGCw'C
Cuts at: 0 448 2625
Size: 448 2177
BsmI GAATG_Cn'
Cuts at: 0 794 2625
Size: 794 1831
Bsp1286I G_dGCh'C
Cuts at: 0 448 716 2625
Size: 448 268 1909
Fragments arranged by size:
1909 448 268
BspEI T'CCGG_A
Cuts at: 0 1763 2625
Size: 1763 862
BspHI T'CATG_A
Cuts at: 0 426 2625
Size: 426 2199
BsrBI CCG'CTC
Cuts at: 0 6 1882 2625
Size: 6 1876 743
Fragments arranged by size:
1876 743 6
BsrDI GCAATG_nn'
Cuts at: 0 1933 2276 2625
Size: 1933 343 349
Fragments arranged by size:
1933 349 343
BsrFI r'CCGG_y
Cuts at: 0 10 320 2625
Size: 10 310 2305
Fragments arranged by size:
2305 310 10
BsrGI T'GTAC_A
Cuts at: 0 1357 1720 2180 2625
Size: 1357 363 460 445
Fragments arranged by size:
1357 460 445 363
BssSI C'ACGA_G
Cuts at: 0 260 2625
Size: 260 2365
BstAPI GCAn_nnn'nTGC
Cuts at: 0 172 2625
Size: 172 2453
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 1557 2625
Size: 156 1401 1068
Fragments arranged by size:
1401 1068 156
BstYI r'GATC_y
Cuts at: 0 741 1505 2241 2625
Size: 741 764 736 384
Fragments arranged by size:
764 741 736 384
BtgI C'CryG_G
Cuts at: 0 528 1544 1971 2625
Size: 528 1016 427 654
Fragments arranged by size:
1016 654 528 427
BtsI GCAGTG_nn'
Cuts at: 0 967 1342 1538 2625
Size: 967 375 196 1087
Fragments arranged by size:
1087 967 375 196
DrdI GACnn_nn'nnGTC
Cuts at: 0 2502 2625
Size: 2502 123
EaeI y'GGCC_r
Cuts at: 0 1547 2607 2625
Size: 1547 1060 18
Fragments arranged by size:
1547 1060 18
EarI CTCTTCn'nnn_
Cuts at: 0 821 2625
Size: 821 1804
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 994 1574 1665 1942 2625
Size: 644 350 580 91 277 683
Fragments arranged by size:
683 644 580 350 277 91
EcoO109I rG'GnC_Cy
Cuts at: 0 2490 2562 2625
Size: 2490 72 63
Fragments arranged by size:
2490 72 63
EcoRV GAT'ATC
Cuts at: 0 539 2625
Size: 539 2086
FseI GG_CCGG'CC
Cuts at: 0 14 2625
Size: 14 2611
HaeII r_GCGC'y
Cuts at: 0 706 2625
Size: 706 1919
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 67 99 143 175 430 462 1561 1593
Size: 67 32 44 32 255 32 1099 32
Cuts at: 1593 1606 1638 2625
Size: 13 32 987
Fragments arranged by size:
1099 987 255 67 44 32 32 32
32 32 13
HincII GTy'rAC
Cuts at: 0 765 2625
Size: 765 1860
HindIII A'AGCT_T
Cuts at: 0 1478 2625
Size: 1478 1147
MscI TGG'CCA
Cuts at: 0 1549 2625
Size: 1549 1076
MslI CAynn'nnrTG
Cuts at: 0 196 488 527 1087 1519 1973 2625
Size: 196 292 39 560 432 454 652
Fragments arranged by size:
652 560 454 432 292 196 39
NaeI GCC'GGC
Cuts at: 0 12 2625
Size: 12 2613
NcoI C'CATG_G
Cuts at: 0 528 1544 2625
Size: 528 1016 1081
Fragments arranged by size:
1081 1016 528
NdeI CA'TA_TG
Cuts at: 0 735 2625
Size: 735 1890
NgoMIV G'CCGG_C
Cuts at: 0 10 2625
Size: 10 2615
NsiI A_TGCA'T
Cuts at: 0 168 735 2625
Size: 168 567 1890
Fragments arranged by size:
1890 567 168
NspI r_CATG'y
Cuts at: 0 697 1230 1852 1875 2625
Size: 697 533 622 23 750
Fragments arranged by size:
750 697 622 533 23
PciI A'CATG_T
Cuts at: 0 693 1226 2625
Size: 693 533 1399
Fragments arranged by size:
1399 693 533
PflMI CCAn_nnn'nTGG
Cuts at: 0 1962 2625
Size: 1962 663
PmlI CAC'GTG
Cuts at: 0 2502 2625
Size: 2502 123
PsiI TTA'TAA
Cuts at: 0 2215 2625
Size: 2215 410
PstI C_TGCA'G
Cuts at: 0 2539 2625
Size: 2539 86
SfcI C'TryA_G
Cuts at: 0 1618 2165 2535 2625
Size: 1618 547 370 90
Fragments arranged by size:
1618 547 370 90
SmlI C'TyrA_G
Cuts at: 0 447 2625
Size: 447 2178
StyI C'CwwG_G
Cuts at: 0 415 528 1544 2625
Size: 415 113 1016 1081
Fragments arranged by size:
1081 1016 415 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 75 2625
Size: 75 2550
TatI w'GTAC_w
Cuts at: 0 1357 1720 2180 2625
Size: 1357 363 460 445
Fragments arranged by size:
1357 460 445 363
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1253 1285 1785 1817 2625
Size: 1253 32 500 32 808
Fragments arranged by size:
1253 808 500 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 85 2625
Size: 85 2540
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 153 1110 2625
Size: 153 957 1515
Fragments arranged by size:
1515 957 153
XmnI GAAnn'nnTTC
Cuts at: 0 1469 2625
Size: 1469 1156
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AloI AlwNI ApoI
AseI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BlpI BmrI BpmI BpuEI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstXI BstYI BtgI BtsI DrdI EaeI EarI Eco57MI
EcoO109I EcoRV FseI HaeII Hin4I HincII HindIII MscI
MslI NaeI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PmlI PsiI PstI SfcI SmlI StyI TaqII
TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
ApaI ApaLI AscI AsiSI AvaI AvrII BaeI BbeI
BbvCI BglI BglII Bme1580I BmgBI BmtI BplI Bpu10I
BsaI BsaHI BsiWI BsmBI BspMI BssHII BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoNI EcoRI FalI FspI FspAI HpaI
KasI KpnI MfeI MluI MmeI MspA1I NarI NheI
NotI NruI PacI PasI PfoI PmeI PpiI PpuMI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI XbaI XhoI XmaI ZraI