Restriction map for
pF1KE0332
(Linear) (Six-base) MAPSORT of: KIEE0332.seq Check: 2687 from: 1 to: 1878
KIEE0332.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 30 939 1878
Size: 30 909 939
Fragments arranged by size:
939 909 30
AflIII A'CryG_T
Cuts at: 0 91 1144 1878
Size: 91 1053 734
Fragments arranged by size:
1053 734 91
AgeI A'CCGG_T
Cuts at: 0 209 1878
Size: 209 1669
AlwNI CAG_nnn'CTG
Cuts at: 0 45 1441 1878
Size: 45 1396 437
Fragments arranged by size:
1396 437 45
ApoI r'AATT_y
Cuts at: 0 1467 1878
Size: 1467 411
AseI AT'TA_AT
Cuts at: 0 829 1878
Size: 829 1049
AvrII C'CTAG_G
Cuts at: 0 375 1878
Size: 375 1503
BamHI G'GATC_C
Cuts at: 0 71 1878
Size: 71 1807
BbsI GAAGACnn'nnnn_
Cuts at: 0 37 537 890 1878
Size: 37 500 353 988
Fragments arranged by size:
988 500 353 37
BciVI GTATCCnnnnn_n'
Cuts at: 0 1278 1878
Size: 1278 600
BmgBI CAC'GTC
Cuts at: 0 27 119 360 552 1878
Size: 27 92 241 192 1326
Fragments arranged by size:
1326 241 192 92 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 1003 1878
Size: 1003 875
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1561 1878
Size: 1561 317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 1295 1324 1556 1635 1725 1878
Size: 207 1088 29 232 79 90 153
Fragments arranged by size:
1088 232 207 153 90 79 29
Bpu10I CC'TnA_GC
Cuts at: 0 1079 1878
Size: 1079 799
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1214 1878
Size: 607 607 664
Fragments arranged by size:
664 607 607
BsaAI yAC'GTr
Cuts at: 0 92 1147 1878
Size: 92 1055 731
Fragments arranged by size:
1055 731 92
BsaHI Gr'CG_yC
Cuts at: 0 14 1878
Size: 14 1864
BsaWI w'CCGG_w
Cuts at: 0 209 1878
Size: 209 1669
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 130 160 658 688 1523 1553 1709 1739
Size: 130 30 498 30 835 30 156 30
Cuts at: 1739 1878
Size: 139
Fragments arranged by size:
835 498 156 139 130 30 30 30
30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 152 563 1751 1878
Size: 58 94 411 1188 127
Fragments arranged by size:
1188 411 127 94 58
BsiEI CG_ry'CG
Cuts at: 0 210 1674 1878
Size: 210 1464 204
Fragments arranged by size:
1464 210 204
BsmI GAATG_Cn'
Cuts at: 0 1198 1328 1878
Size: 1198 130 550
Fragments arranged by size:
1198 550 130
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 30 939 1878
Size: 30 909 939
Fragments arranged by size:
939 909 30
BsrDI GCAATG_nn'
Cuts at: 0 1157 1878
Size: 1157 721
BsrFI r'CCGG_y
Cuts at: 0 209 1878
Size: 209 1669
BssSI C'ACGA_G
Cuts at: 0 535 1878
Size: 535 1343
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 1878
Size: 45 1833
BstBI TT'CG_AA
Cuts at: 0 1422 1878
Size: 1422 456
BstYI r'GATC_y
Cuts at: 0 71 1308 1878
Size: 71 1237 570
Fragments arranged by size:
1237 570 71
Bsu36I CC'TnA_GG
Cuts at: 0 301 1878
Size: 301 1577
BtgI C'CryG_G
Cuts at: 0 1500 1878
Size: 1500 378
BtsI GCAGTG_nn'
Cuts at: 0 135 1781 1878
Size: 135 1646 97
Fragments arranged by size:
1646 135 97
DraIII CAC_nnn'GTG
Cuts at: 0 122 1878
Size: 122 1756
EaeI y'GGCC_r
Cuts at: 0 5 108 654 1479 1671 1878
Size: 5 103 546 825 192 207
Fragments arranged by size:
825 546 207 192 103 5
EagI C'GGCC_G
Cuts at: 0 1671 1878
Size: 1671 207
EarI CTCTTCn'nnn_
Cuts at: 0 160 1878
Size: 160 1718
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1486 1878
Size: 1486 392
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 207 1295 1324 1556 1635 1725 1878
Size: 207 1088 29 232 79 90 153
Fragments arranged by size:
1088 232 207 153 90 79 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 379 430 1878
Size: 379 51 1448
Fragments arranged by size:
1448 379 51
EcoO109I rG'GnC_Cy
Cuts at: 0 371 1878
Size: 371 1507
EcoRV GAT'ATC
Cuts at: 0 1755 1878
Size: 1755 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 457 489 945 977 1273 1305
Size: 130 32 295 32 456 32 296 32
Cuts at: 1305 1351 1383 1878
Size: 46 32 495
Fragments arranged by size:
495 456 296 295 130 46 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 357 1878
Size: 357 1521
MfeI C'AATT_G
Cuts at: 0 818 1335 1878
Size: 818 517 543
Fragments arranged by size:
818 543 517
MscI TGG'CCA
Cuts at: 0 7 110 656 1481 1878
Size: 7 103 546 825 397
Fragments arranged by size:
825 546 397 103 7
MspA1I CmG'CkG
Cuts at: 0 136 1735 1878
Size: 136 1599 143
Fragments arranged by size:
1599 143 136
NdeI CA'TA_TG
Cuts at: 0 972 1878
Size: 972 906
NspI r_CATG'y
Cuts at: 0 235 401 1381 1878
Size: 235 166 980 497
Fragments arranged by size:
980 497 235 166
PflMI CCAn_nnn'nTGG
Cuts at: 0 663 1878
Size: 663 1215
PmlI CAC'GTG
Cuts at: 0 92 1147 1878
Size: 92 1055 731
Fragments arranged by size:
1055 731 92
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1004 1036 1878
Size: 1004 32 842
Fragments arranged by size:
1004 842 32
PpuMI rG'GwC_Cy
Cuts at: 0 371 1878
Size: 371 1507
PstI C_TGCA'G
Cuts at: 0 130 1446 1878
Size: 130 1316 432
Fragments arranged by size:
1316 432 130
PvuII CAG'CTG
Cuts at: 0 1735 1878
Size: 1735 143
SanDI GG'GwC_CC
Cuts at: 0 371 1878
Size: 371 1507
SfcI C'TryA_G
Cuts at: 0 126 722 1442 1698 1878
Size: 126 596 720 256 180
Fragments arranged by size:
720 596 256 180 126
SmlI C'TyrA_G
Cuts at: 0 586 1229 1878
Size: 586 643 649
Fragments arranged by size:
649 643 586
SpeI A'CTAG_T
Cuts at: 0 1804 1878
Size: 1804 74
SphI G_CATG'C
Cuts at: 0 1381 1878
Size: 1381 497
SspI AAT'ATT
Cuts at: 0 762 1878
Size: 762 1116
StuI AGG'CCT
Cuts at: 0 670 1878
Size: 670 1208
StyI C'CwwG_G
Cuts at: 0 375 1403 1487 1878
Size: 375 1028 84 391
Fragments arranged by size:
1028 391 375 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 249 259 1878
Size: 249 10 1619
Fragments arranged by size:
1619 249 10
TatI w'GTAC_w
Cuts at: 0 1339 1878
Size: 1339 539
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 658 690 779 811 1791 1823 1878
Size: 658 32 89 32 980 32 55
Fragments arranged by size:
980 658 89 55 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1626 1878
Size: 1626 252
Enzymes that do cut:
AarI AflIII AgeI AlwNI ApoI AseI AvrII BamHI
BbsI BciVI BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsmI BspMI
BsrDI BsrFI BssSI BstAPI BstBI BstYI Bsu36I BtgI
BtsI DraIII EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRV Hin4I HincII MfeI MscI MspA1I NdeI
NspI PflMI PmlI PpiI PpuMI PstI PvuII SanDI
SfcI SmlI SpeI SphI SspI StuI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
BaeI BanI BanII BbeI BbvCI BcgI BclI BfrBI
BglI BglII BlpI Bme1580I BmtI BsaI BsaBI BseRI
BseYI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI
BsrGI BssHII BstEII BstXI BstZ17I BtgZI ClaI CspCI
DraI DrdI EcoICRI EcoRI FalI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MluI MmeI MslI
NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SrfI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI