Restriction map for
pF1KE0302
(Linear) (Six-base) MAPSORT of: KIEE0302.seq Check: 6066 from: 1 to: 1428
KIEE0302.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
Acc65I G'GTAC_C
Cuts at: 0 929 1428
Size: 929 499
AfeI AGC'GCT
Cuts at: 0 118 1428
Size: 118 1310
AflIII A'CryG_T
Cuts at: 0 420 1428
Size: 420 1008
AleI CACnn'nnGTG
Cuts at: 0 52 1428
Size: 52 1376
AlwNI CAG_nnn'CTG
Cuts at: 0 383 1428
Size: 383 1045
ApaLI G'TGCA_C
Cuts at: 0 274 1428
Size: 274 1154
AvaI C'yCGr_G
Cuts at: 0 102 1091 1099 1428
Size: 102 989 8 329
Fragments arranged by size:
989 329 102 8
BamHI G'GATC_C
Cuts at: 0 725 849 1428
Size: 725 124 579
Fragments arranged by size:
725 579 124
BanI G'GyrC_C
Cuts at: 0 929 1428
Size: 929 499
BanII G_rGCy'C
Cuts at: 0 61 73 109 648 1213 1291 1428
Size: 61 12 36 539 565 78 137
Fragments arranged by size:
565 539 137 78 61 36 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 32 423 772 1428
Size: 32 391 349 656
Fragments arranged by size:
656 391 349 32
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 32 873 907 1428
Size: 32 841 34 521
Fragments arranged by size:
841 521 34 32
BciVI GTATCCnnnnn_n'
Cuts at: 0 298 1428
Size: 298 1130
BclI T'GATC_A
Cuts at: 0 254 1316 1428
Size: 254 1062 112
Fragments arranged by size:
1062 254 112
BfrBI ATG'CAT
Cuts at: 0 1160 1428
Size: 1160 268
Bme1580I G_kGCm'C
Cuts at: 0 278 1428
Size: 278 1150
BmgBI CAC'GTC
Cuts at: 0 54 421 1428
Size: 54 367 1007
Fragments arranged by size:
1007 367 54
BmrI ACTGGGnnnn_n'
Cuts at: 0 420 1428
Size: 420 1008
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 1276 1428
Size: 1030 246 152
Fragments arranged by size:
1030 246 152
BsaBI GATnn'nnATC
Cuts at: 0 1072 1321 1428
Size: 1072 249 107
Fragments arranged by size:
1072 249 107
BsaHI Gr'CG_yC
Cuts at: 0 565 1310 1428
Size: 565 745 118
Fragments arranged by size:
745 565 118
BsaWI w'CCGG_w
Cuts at: 0 395 1428
Size: 395 1033
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 58 79 82 88 109 112 580
Size: 28 30 21 3 6 21 3 468
Cuts at: 580 610 1428
Size: 30 818
Fragments arranged by size:
818 468 30 30 28 21 21 6
3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 87 1428
Size: 79 8 1341
Fragments arranged by size:
1341 79 8
BseYI C'CCAG_C
Cuts at: 0 449 453 736 1428
Size: 449 4 283 692
Fragments arranged by size:
692 449 283 4
BsiHKAI G_wGCw'C
Cuts at: 0 278 306 1106 1428
Size: 278 28 800 322
Fragments arranged by size:
800 322 278 28
BsmBI CGTCTCn'nnnn_
Cuts at: 0 544 1428
Size: 544 884
Bsp1286I G_dGCh'C
Cuts at: 0 61 73 109 278 306 648 1106 1213
Size: 61 12 36 169 28 342 458 107
Cuts at: 1213 1291 1428
Size: 78 137
Fragments arranged by size:
458 342 169 137 107 78 61 36
28 12
BspEI T'CCGG_A
Cuts at: 0 395 1428
Size: 395 1033
BspHI T'CATG_A
Cuts at: 0 698 1428
Size: 698 730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 941 983 1428
Size: 941 42 445
Fragments arranged by size:
941 445 42
BsrBI CCG'CTC
Cuts at: 0 1251 1428
Size: 1251 177
BsrDI GCAATG_nn'
Cuts at: 0 1395 1428
Size: 1395 33
BstEII G'GTnAC_C
Cuts at: 0 1260 1428
Size: 1260 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 121 1428
Size: 121 1307
BstYI r'GATC_y
Cuts at: 0 725 849 1025 1428
Size: 725 124 176 403
Fragments arranged by size:
725 403 176 124
Bsu36I CC'TnA_GG
Cuts at: 0 62 1428
Size: 62 1366
BtgI C'CryG_G
Cuts at: 0 47 572 1428
Size: 47 525 856
Fragments arranged by size:
856 525 47
BtsI GCAGTG_nn'
Cuts at: 0 276 1428
Size: 276 1152
DraI TTT'AAA
Cuts at: 0 498 1428
Size: 498 930
EaeI y'GGCC_r
Cuts at: 0 269 515 668 686 1166 1428
Size: 269 246 153 18 480 262
Fragments arranged by size:
480 269 262 246 153 18
EarI CTCTTCn'nnn_
Cuts at: 0 784 1428
Size: 784 644
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1030 1276 1428
Size: 1030 246 152
Fragments arranged by size:
1030 246 152
EcoO109I rG'GnC_Cy
Cuts at: 0 238 1352 1428
Size: 238 1114 76
Fragments arranged by size:
1114 238 76
FspI TGC'GCA
Cuts at: 0 1135 1428
Size: 1135 293
FspAI rTGC'GCAy
Cuts at: 0 1135 1428
Size: 1135 293
HaeII r_GCGC'y
Cuts at: 0 120 1099 1229 1428
Size: 120 979 130 199
Fragments arranged by size:
979 199 130 120
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 58 60 90 759 791 935 967
Size: 28 30 2 30 669 32 144 32
Cuts at: 967 1428
Size: 461
Fragments arranged by size:
669 461 144 32 32 30 30 28
2
KpnI G_GTAC'C
Cuts at: 0 933 1428
Size: 933 495
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 1428
Size: 56 1372
MscI TGG'CCA
Cuts at: 0 670 688 1168 1428
Size: 670 18 480 260
Fragments arranged by size:
670 480 260 18
MslI CAynn'nnrTG
Cuts at: 0 52 232 545 679 817 1428
Size: 52 180 313 134 138 611
Fragments arranged by size:
611 313 180 138 134 52
MspA1I CmG'CkG
Cuts at: 0 453 1428
Size: 453 975
NcoI C'CATG_G
Cuts at: 0 572 1428
Size: 572 856
NruI TCG'CGA
Cuts at: 0 582 1428
Size: 582 846
NsiI A_TGCA'T
Cuts at: 0 1162 1428
Size: 1162 266
NspI r_CATG'y
Cuts at: 0 594 1160 1428
Size: 594 566 268
Fragments arranged by size:
594 566 268
PflMI CCAn_nnn'nTGG
Cuts at: 0 986 1428
Size: 986 442
PfoI T'CCnGG_A
Cuts at: 0 1253 1428
Size: 1253 175
PpuMI rG'GwC_Cy
Cuts at: 0 238 1352 1428
Size: 238 1114 76
Fragments arranged by size:
1114 238 76
PvuII CAG'CTG
Cuts at: 0 453 1428
Size: 453 975
SanDI GG'GwC_CC
Cuts at: 0 238 1428
Size: 238 1190
SexAI A'CCwGG_T
Cuts at: 0 1190 1428
Size: 1190 238
SfcI C'TryA_G
Cuts at: 0 39 1428
Size: 39 1389
SmaI CCC'GGG
Cuts at: 0 104 1428
Size: 104 1324
StuI AGG'CCT
Cuts at: 0 83 1428
Size: 83 1345
StyI C'CwwG_G
Cuts at: 0 572 749 760 954 1428
Size: 572 177 11 194 474
Fragments arranged by size:
572 474 194 177 11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 463 1428
Size: 463 965
TatI w'GTAC_w
Cuts at: 0 1013 1428
Size: 1013 415
Tth111I GACn'n_nGTC
Cuts at: 0 1192 1428
Size: 1192 236
XmaI C'CCGG_G
Cuts at: 0 102 1428
Size: 102 1326
Enzymes that do cut:
Acc65I AfeI AflIII AleI AlwNI ApaLI AvaI BamHI
BanI BanII BbsI BcgI BciVI BclI BfrBI Bme1580I
BmgBI BmrI BpmI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BstEII BstXI BstYI Bsu36I BtgI BtsI DraI
EaeI EarI Eco57MI EcoO109I FspI FspAI HaeII Hin4I
KpnI MmeI MscI MslI MspA1I NcoI NruI NsiI
NspI PflMI PfoI PpuMI PvuII SanDI SexAI SfcI
SmaI StuI StyI TaqII TatI Tth111I XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AhdI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BglI BglII BlpI BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsgI BsiEI BsiWI BsmI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DraIII DrdI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI HincII HindIII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TstI
XbaI XcmI XhoI XmnI ZraI