Restriction map for
pF1KE0295
(Linear) (Six-base) MAPSORT of: KIEE0295.seq Check: 123 from: 1 to: 708
KIEE0295.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
ApaI G_GGCC'C
Cuts at: 0 332 492 660 708
Size: 332 160 168 48
Fragments arranged by size:
332 168 160 48
ApaLI G'TGCA_C
Cuts at: 0 580 708
Size: 580 128
AvaI C'yCGr_G
Cuts at: 0 14 73 161 416 485 491 708
Size: 14 59 88 255 69 6 217
Fragments arranged by size:
255 217 88 69 59 14 6
BanI G'GyrC_C
Cuts at: 0 9 145 283 395 403 481 708
Size: 9 136 138 112 8 78 227
Fragments arranged by size:
227 138 136 112 78 9 8
BanII G_rGCy'C
Cuts at: 0 59 74 332 492 660 671 708
Size: 59 15 258 160 168 11 37
Fragments arranged by size:
258 168 160 59 37 15 11
BbeI G_GCGC'C
Cuts at: 0 287 399 708
Size: 287 112 309
Fragments arranged by size:
309 287 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 24 708
Size: 24 684
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 127 161 708
Size: 127 34 547
Fragments arranged by size:
547 127 34
Bme1580I G_kGCm'C
Cuts at: 0 150 196 332 408 492 584 660 708
Size: 150 46 136 76 84 92 76 48
Fragments arranged by size:
150 136 92 84 76 76 48 46
BmgBI CAC'GTC
Cuts at: 0 579 708
Size: 579 129
BmrI ACTGGGnnnn_n'
Cuts at: 0 56 62 708
Size: 56 6 646
Fragments arranged by size:
646 56 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 708
Size: 139 569
Bpu10I CC'TnA_GC
Cuts at: 0 238 313 708
Size: 238 75 395
Fragments arranged by size:
395 238 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 16 708
Size: 16 692
BsaHI Gr'CG_yC
Cuts at: 0 284 396 708
Size: 284 112 312
Fragments arranged by size:
312 284 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 97 127 708
Size: 97 30 581
Fragments arranged by size:
581 97 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 102 708
Size: 102 606
BseYI C'CCAG_C
Cuts at: 0 694 708
Size: 694 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 589 708
Size: 589 119
BsiHKAI G_wGCw'C
Cuts at: 0 134 584 708
Size: 134 450 124
Fragments arranged by size:
450 134 124
Bsp1286I G_dGCh'C
Cuts at: 0 59 74 134 150 196 332 408 492
Size: 59 15 60 16 46 136 76 84
Cuts at: 492 584 660 671 708
Size: 92 76 11 37
Fragments arranged by size:
136 92 84 76 76 60 59 46
37 16 15 11
BsrBI CCG'CTC
Cuts at: 0 110 708
Size: 110 598
BsrFI r'CCGG_y
Cuts at: 0 232 444 708
Size: 232 212 264
Fragments arranged by size:
264 232 212
BstAPI GCAn_nnn'nTGC
Cuts at: 0 588 708
Size: 588 120
BtgI C'CryG_G
Cuts at: 0 287 477 648 708
Size: 287 190 171 60
Fragments arranged by size:
287 190 171 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 273 708
Size: 273 435
BtsI GCAGTG_nn'
Cuts at: 0 209 249 708
Size: 209 40 459
Fragments arranged by size:
459 209 40
DraIII CAC_nnn'GTG
Cuts at: 0 185 708
Size: 185 523
EarI CTCTTCn'nnn_
Cuts at: 0 529 708
Size: 529 179
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 708
Size: 139 569
EcoO109I rG'GnC_Cy
Cuts at: 0 165 249 328 456 708
Size: 165 84 79 128 252
Fragments arranged by size:
252 165 128 84 79
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 291 708
Size: 291 417
HaeII r_GCGC'y
Cuts at: 0 204 287 399 708
Size: 204 83 112 309
Fragments arranged by size:
309 204 112 83
KasI G'GCGC_C
Cuts at: 0 283 395 708
Size: 283 112 313
Fragments arranged by size:
313 283 112
NaeI GCC'GGC
Cuts at: 0 446 708
Size: 446 262
NarI GG'CG_CC
Cuts at: 0 284 396 708
Size: 284 112 312
Fragments arranged by size:
312 284 112
NgoMIV G'CCGG_C
Cuts at: 0 444 708
Size: 444 264
NspI r_CATG'y
Cuts at: 0 192 708
Size: 192 516
PfoI T'CCnGG_A
Cuts at: 0 35 167 708
Size: 35 132 541
Fragments arranged by size:
541 132 35
PpuMI rG'GwC_Cy
Cuts at: 0 165 708
Size: 165 543
PshAI GACnn'nnGTC
Cuts at: 0 28 708
Size: 28 680
PspOMI G'GGCC_C
Cuts at: 0 328 488 656 708
Size: 328 160 168 52
Fragments arranged by size:
328 168 160 52
PstI C_TGCA'G
Cuts at: 0 305 708
Size: 305 403
SfcI C'TryA_G
Cuts at: 0 301 708
Size: 301 407
SfoI GGC'GCC
Cuts at: 0 285 397 708
Size: 285 112 311
Fragments arranged by size:
311 285 112
SmaI CCC'GGG
Cuts at: 0 16 163 418 487 493 708
Size: 16 147 255 69 6 215
Fragments arranged by size:
255 215 147 69 16 6
StuI AGG'CCT
Cuts at: 0 307 708
Size: 307 401
StyI C'CwwG_G
Cuts at: 0 215 708
Size: 215 493
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 391 641 708
Size: 391 250 67
Fragments arranged by size:
391 250 67
TatI w'GTAC_w
Cuts at: 0 674 708
Size: 674 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 97 129 708
Size: 97 32 579
Fragments arranged by size:
579 97 32
Tth111I GACn'n_nGTC
Cuts at: 0 424 708
Size: 424 284
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 339 708
Size: 339 369
XmaI C'CCGG_G
Cuts at: 0 14 161 416 485 491 708
Size: 14 147 255 69 6 217
Fragments arranged by size:
255 217 147 69 14 6
Enzymes that do cut:
ApaI ApaLI AvaI BanI BanII BbeI BbsI BcgI
Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrFI BstAPI
BtgI BtgZI BtsI DraIII EarI Eco57MI EcoO109I FalI
HaeII KasI NaeI NarI NgoMIV NspI PfoI PpuMI
PshAI PspOMI PstI SfcI SfoI SmaI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BclI BfrBI BglI BglII BlpI BmtI BplI BpuEI
BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EaeI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI XmnI ZraI