(Linear) (Six-base) MAPSORT of: KIEE0289.seq  Check: 8835  from: 1  to: 936



KIEE0289.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

Acc65I G'GTAC_C

Cuts at:      0     757     936 
   Size:        757     179
 
AseI AT'TA_AT

Cuts at:      0     469     936 
   Size:        469     467
 
BamHI G'GATC_C

Cuts at:      0     680     936 
   Size:        680     256
 
BanI G'GyrC_C

Cuts at:      0     757     936 
   Size:        757     179
 
BclI T'GATC_A

Cuts at:      0     641     936 
   Size:        641     295
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     786     936 
   Size:        786     150
 
BmtI G_CTAG'C

Cuts at:      0     674     936 
   Size:        674     262
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     507     936 
   Size:        507     429
 
BsaWI w'CCGG_w

Cuts at:      0     607     936 
   Size:        607     329
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     504     936 
   Size:        504     432
 
BspEI T'CCGG_A

Cuts at:      0     607     936 
   Size:        607     329
 
BsrDI GCAATG_nn'

Cuts at:      0     614     659     936 
   Size:        614      45     277

  Fragments arranged by size: 

                 614     277      45
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     243     936 
   Size:        243     693
 
BstYI r'GATC_y

Cuts at:      0     680     936 
   Size:        680     256
 
Bsu36I CC'TnA_GG

Cuts at:      0     676     936 
   Size:        676     260
 
BtgI C'CryG_G

Cuts at:      0     620     936 
   Size:        620     316
 
BtsI GCAGTG_nn'

Cuts at:      0     547     905     936 
   Size:        547     358      31

  Fragments arranged by size: 

                 547     358      31
 
EaeI y'GGCC_r

Cuts at:      0     371     623     936 
   Size:        371     252     313

  Fragments arranged by size: 

                 371     313     252
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     117     504     936 
   Size:        117     387     432

  Fragments arranged by size: 

                 432     387     117
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      94     936 
   Size:         94     842
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     305     337     936 
   Size:        305      32     599

  Fragments arranged by size: 

                 599     305      32
 
HincII GTy'rAC

Cuts at:      0     462     599     936 
   Size:        462     137     337

  Fragments arranged by size: 

                 462     337     137
 
HpaI GTT'AAC

Cuts at:      0     462     599     936 
   Size:        462     137     337

  Fragments arranged by size: 

                 462     337     137
 
KpnI G_GTAC'C

Cuts at:      0     761     936 
   Size:        761     175
 
MfeI C'AATT_G

Cuts at:      0     227     936 
   Size:        227     709
 
MscI TGG'CCA

Cuts at:      0     373     625     936 
   Size:        373     252     311

  Fragments arranged by size: 

                 373     311     252
 
MslI CAynn'nnrTG

Cuts at:      0     172     577     619     916     936 
   Size:        172     405      42     297      20

  Fragments arranged by size: 

                 405     297     172      42      20
 
MspA1I CmG'CkG

Cuts at:      0     191     936 
   Size:        191     745
 
NcoI C'CATG_G

Cuts at:      0     620     936 
   Size:        620     316
 
NheI G'CTAG_C

Cuts at:      0     670     936 
   Size:        670     266
 
PvuII CAG'CTG

Cuts at:      0     191     936 
   Size:        191     745
 
SfcI C'TryA_G

Cuts at:      0     398     936 
   Size:        398     538
 
SmlI C'TyrA_G

Cuts at:      0     486     936 
   Size:        486     450
 
StyI C'CwwG_G

Cuts at:      0     620     936 
   Size:        620     316
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     186     249     936 
   Size:        186      63     687

  Fragments arranged by size: 

                 687     186      63
 
TatI w'GTAC_w

Cuts at:      0     176     936 
   Size:        176     760
 
XmnI GAAnn'nnTTC

Cuts at:      0     925     936 
   Size:        925      11
 

 Enzymes that do cut:

   Acc65I     AseI    BamHI     BanI     BclI     BmrI     BmtI    BpuEI
    BsaWI    BseRI    BspEI    BsrDI    BstXI    BstYI   Bsu36I     BtgI
     BtsI     EaeI    EcoNI     FalI    Hin4I   HincII     HpaI     KpnI
     MfeI     MscI     MslI   MspA1I     NcoI     NheI    PvuII     SfcI
     SmlI     StyI    TaqII     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI    AsiSI     AvaI    AvrII     BaeI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII  HindIII
     KasI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI