(Linear) (Six-base) MAPSORT of: KIEE0277.seq  Check: 5837  from: 1  to: 963



KIEE0277.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      11     963 
   Size:         11     952
 
AflII C'TTAA_G

Cuts at:      0     691     963 
   Size:        691     272
 
AgeI A'CCGG_T

Cuts at:      0     389     963 
   Size:        389     574
 
AleI CACnn'nnGTG

Cuts at:      0     761     805     963 
   Size:        761      44     158

  Fragments arranged by size: 

                 761     158      44
 
ApaLI G'TGCA_C

Cuts at:      0     808     963 
   Size:        808     155
 
ApoI r'AATT_y

Cuts at:      0      51     517     963 
   Size:         51     466     446

  Fragments arranged by size: 

                 466     446      51
 
BamHI G'GATC_C

Cuts at:      0     275     963 
   Size:        275     688
 
BanII G_rGCy'C

Cuts at:      0     464     963 
   Size:        464     499
 
BlpI GC'TnA_GC

Cuts at:      0     585     963 
   Size:        585     378
 
Bme1580I G_kGCm'C

Cuts at:      0     812     963 
   Size:        812     151
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     318     963 
   Size:        318     645
 
BsaWI w'CCGG_w

Cuts at:      0     389     963 
   Size:        389     574
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     111     361     963 
   Size:        111     250     602

  Fragments arranged by size: 

                 602     250     111
 
BseYI C'CCAG_C

Cuts at:      0     270     963 
   Size:        270     693
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     550     963 
   Size:        175     375     413

  Fragments arranged by size: 

                 413     375     175
 
BsiHKAI G_wGCw'C

Cuts at:      0     812     963 
   Size:        812     151
 
BsmI GAATG_Cn'

Cuts at:      0     938     963 
   Size:        938      25
 
Bsp1286I G_dGCh'C

Cuts at:      0     464     812     963 
   Size:        464     348     151

  Fragments arranged by size: 

                 464     348     151
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     754     963 
   Size:        754     209
 
BsrDI GCAATG_nn'

Cuts at:      0     317     395     963 
   Size:        317      78     568

  Fragments arranged by size: 

                 568     317      78
 
BsrFI r'CCGG_y

Cuts at:      0     389     963 
   Size:        389     574
 
BsrGI T'GTAC_A

Cuts at:      0     260     963 
   Size:        260     703
 
BstYI r'GATC_y

Cuts at:      0     275     573     963 
   Size:        275     298     390

  Fragments arranged by size: 

                 390     298     275
 
BtgI C'CryG_G

Cuts at:      0     134     339     836     963 
   Size:        134     205     497     127

  Fragments arranged by size: 

                 497     205     134     127
 
EarI CTCTTCn'nnn_

Cuts at:      0     716     963 
   Size:        716     247
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      11     963 
   Size:         11     952
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     568     963 
   Size:        568     395
 
EcoRI G'AATT_C

Cuts at:      0      51     963 
   Size:         51     912
 
MfeI C'AATT_G

Cuts at:      0     150     963 
   Size:        150     813
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     632     963 
   Size:        632     331
 
MslI CAynn'nnrTG

Cuts at:      0     761     805     963 
   Size:        761      44     158

  Fragments arranged by size: 

                 761     158      44
 
NcoI C'CATG_G

Cuts at:      0     134     339     963 
   Size:        134     205     624

  Fragments arranged by size: 

                 624     205     134
 
SfcI C'TryA_G

Cuts at:      0     900     963 
   Size:        900      63
 
SmlI C'TyrA_G

Cuts at:      0     691     963 
   Size:        691     272
 
StyI C'CwwG_G

Cuts at:      0     134     339     963 
   Size:        134     205     624

  Fragments arranged by size: 

                 624     205     134
 
TatI w'GTAC_w

Cuts at:      0     260     963 
   Size:        260     703
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     141     850     963 
   Size:        141     709     113

  Fragments arranged by size: 

                 709     141     113
 

 Enzymes that do cut:

     AcuI    AflII     AgeI     AleI    ApaLI     ApoI    BamHI    BanII
     BlpI Bme1580I     BmrI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI
     BsmI Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI    BstYI     BtgI
     EarI  Eco57MI    EcoNI    EcoRI     MfeI     MmeI     MslI     NcoI
     SfcI     SmlI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AhdI
     AlfI     AloI    AlwNI     ApaI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaXI    BsiEI
    BsiWI    BsmBI    BspEI    BspHI    BsrBI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI