(Linear) (Six-base) MAPSORT of: KIEE0274.seq  Check: 3765  from: 1  to: 327



KIEE0274.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AatII G_ACGT'C

Cuts at:      0     100     327 
   Size:        100     227
 
AfeI AGC'GCT

Cuts at:      0      44     327 
   Size:         44     283
 
AflII C'TTAA_G

Cuts at:      0     190     327 
   Size:        190     137
 
AleI CACnn'nnGTG

Cuts at:      0      13     327 
   Size:         13     314
 
ApoI r'AATT_y

Cuts at:      0      85     304     327 
   Size:         85     219      23

  Fragments arranged by size: 

                 219      85      23
 
BanI G'GyrC_C

Cuts at:      0     220     327 
   Size:        220     107
 
BmgBI CAC'GTC

Cuts at:      0      15     327 
   Size:         15     312
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     187     327 
   Size:         31     156     140

  Fragments arranged by size: 

                 156     140      31
 
BsaHI Gr'CG_yC

Cuts at:      0      97     327 
   Size:         97     230
 
BsaWI w'CCGG_w

Cuts at:      0     143     327 
   Size:        143     184
 
BsiHKAI G_wGCw'C

Cuts at:      0      20     327 
   Size:         20     307
 
BsmI GAATG_Cn'

Cuts at:      0      58     321     327 
   Size:         58     263       6

  Fragments arranged by size: 

                 263      58       6
 
Bsp1286I G_dGCh'C

Cuts at:      0      20     327 
   Size:         20     307
 
BsrBI CCG'CTC

Cuts at:      0     167     327 
   Size:        167     160
 
BsrDI GCAATG_nn'

Cuts at:      0      32     327 
   Size:         32     295
 
EarI CTCTTCn'nnn_

Cuts at:      0     179     327 
   Size:        179     148
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     283     327 
   Size:        283      44
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     187     327 
   Size:         31     156     140

  Fragments arranged by size: 

                 156     140      31
 
EcoRI G'AATT_C

Cuts at:      0     304     327 
   Size:        304      23
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     205     327 
   Size:        205     122
 
HaeII r_GCGC'y

Cuts at:      0      46     327 
   Size:         46     281
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     156     188     327 
   Size:        156      32     139

  Fragments arranged by size: 

                 156     139      32
 
MslI CAynn'nnrTG

Cuts at:      0      13     327 
   Size:         13     314
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     315     327 
   Size:        315      12
 
PfoI T'CCnGG_A

Cuts at:      0      47     308     327 
   Size:         47     261      19

  Fragments arranged by size: 

                 261      47      19
 
SapI GCTCTTCn'nnn_

Cuts at:      0     179     327 
   Size:        179     148
 
SmlI C'TyrA_G

Cuts at:      0     190     327 
   Size:        190     137
 
StuI AGG'CCT

Cuts at:      0     104     327 
   Size:        104     223
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     250     327 
   Size:        250      77
 
Tth111I GACn'n_nGTC

Cuts at:      0     237     327 
   Size:        237      90
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     255     327 
   Size:        255      72
 
ZraI GAC'GTC

Cuts at:      0      98     327 
   Size:         98     229
 

 Enzymes that do cut:

    AatII     AfeI    AflII     AleI     ApoI     BanI    BmgBI     BpmI
    BsaHI    BsaWI  BsiHKAI     BsmI Bsp1286I    BsrBI    BsrDI     EarI
     EciI  Eco57MI    EcoRI     FalI    HaeII    Hin4I     MslI    PflMI
     PfoI     SapI     SmlI     StuI    TaqII  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI
    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TatI     TstI
     XbaI     XhoI     XmaI     XmnI