(Linear) (Six-base) MAPSORT of: KIEE0258.seq  Check: 9113  from: 1  to: 414



KIEE0258.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

ApaI G_GGCC'C

Cuts at:      0     113     126     183     414 
   Size:        113      13      57     231

  Fragments arranged by size: 

                 231     113      57      13
 
AvaI C'yCGr_G

Cuts at:      0     158     197     266     317     414 
   Size:        158      39      69      51      97

  Fragments arranged by size: 

                 158      97      69      51      39
 
BanI G'GyrC_C

Cuts at:      0     163     210     292     414 
   Size:        163      47      82     122

  Fragments arranged by size: 

                 163     122      82      47
 
BanII G_rGCy'C

Cuts at:      0     113     126     183     273     313     366     414 
   Size:        113      13      57      90      40      53      48

  Fragments arranged by size: 

                 113      90      57      53      48      40      13
 
Bme1580I G_kGCm'C

Cuts at:      0     113     126     168     183     215     414 
   Size:        113      13      42      15      32     199

  Fragments arranged by size: 

                 199     113      42      32      15      13
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     119     414 
   Size:        119     295
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     354     414 
   Size:        354      60
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      41     414 
   Size:         41     373
 
BseYI C'CCAG_C

Cuts at:      0     312     414 
   Size:        312     102
 
BsiEI CG_ry'CG

Cuts at:      0     117     414 
   Size:        117     297
 
BsiHKAI G_wGCw'C

Cuts at:      0     243     273     414 
   Size:        243      30     141

  Fragments arranged by size: 

                 243     141      30
 
Bsp1286I G_dGCh'C

Cuts at:      0     113     126     168     183     215     243     273     313 
   Size:        113      13      42      15      32      28      30      40

Cuts at:    313     366     414 
   Size:         53      48

  Fragments arranged by size: 

                 113      53      48      42      40      32      30      28
                  15      13
 
BsrBI CCG'CTC

Cuts at:      0      67     414 
   Size:         67     347
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     399     414 
   Size:        399      15
 
BtsI GCAGTG_nn'

Cuts at:      0      27     414 
   Size:         27     387
 
EaeI y'GGCC_r

Cuts at:      0      11     114     414 
   Size:         11     103     300

  Fragments arranged by size: 

                 300     103      11
 
EagI C'GGCC_G

Cuts at:      0     114     414 
   Size:        114     300
 
EcoICRI GAG'CTC

Cuts at:      0     271     414 
   Size:        271     143
 
EcoO109I rG'GnC_Cy

Cuts at:      0     122     180     201     414 
   Size:        122      58      21     213

  Fragments arranged by size: 

                 213     122      58      21
 
HaeII r_GCGC'y

Cuts at:      0     143     414 
   Size:        143     271
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     332     364     414 
   Size:        332      32      50

  Fragments arranged by size: 

                 332      50      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     414 
   Size:         94     320
 
MspA1I CmG'CkG

Cuts at:      0      19     414 
   Size:         19     395
 
PpuMI rG'GwC_Cy

Cuts at:      0     201     414 
   Size:        201     213
 
PspOMI G'GGCC_C

Cuts at:      0     109     122     179     414 
   Size:        109      13      57     235

  Fragments arranged by size: 

                 235     109      57      13
 
PvuII CAG'CTG

Cuts at:      0      19     414 
   Size:         19     395
 
SacI G_AGCT'C

Cuts at:      0     273     414 
   Size:        273     141
 
SmaI CCC'GGG

Cuts at:      0     160     319     414 
   Size:        160     159      95

  Fragments arranged by size: 

                 160     159      95
 
SrfI GCCC'GGGC

Cuts at:      0     319     414 
   Size:        319      95
 
StuI AGG'CCT

Cuts at:      0      99     414 
   Size:         99     315
 
XmaI C'CCGG_G

Cuts at:      0     158     317     414 
   Size:        158     159      97

  Fragments arranged by size: 

                 159     158      97
 

 Enzymes that do cut:

     ApaI     AvaI     BanI    BanII Bme1580I     BplI     BsaI    BseRI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BsrBI    BstXI     BtsI     EaeI
     EagI  EcoICRI EcoO109I    HaeII    Hin4I     MmeI   MspA1I    PpuMI
   PspOMI    PvuII     SacI     SmaI     SrfI     StuI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EarI     EciI  Eco57MI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI
     SphI     SspI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmnI     ZraI