(Linear) (Six-base) MAPSORT of: KIEE0256.seq  Check: 5517  from: 1  to: 1197



KIEE0256.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     402    1197 
   Size:        107     295     795

  Fragments arranged by size: 

                 795     295     107
 
AflIII A'CryG_T

Cuts at:      0     335    1197 
   Size:        335     862
 
AleI CACnn'nnGTG

Cuts at:      0     730    1197 
   Size:        730     467
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      39    1197 
   Size:         39    1158
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     845     877    1197 
   Size:        845      32     320

  Fragments arranged by size: 

                 845     320      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     275    1197 
   Size:        275     922
 
ApoI r'AATT_y

Cuts at:      0     179    1056    1151    1197 
   Size:        179     877      95      46

  Fragments arranged by size: 

                 877     179      95      46
 
BanI G'GyrC_C

Cuts at:      0     520    1197 
   Size:        520     677
 
BanII G_rGCy'C

Cuts at:      0     885    1197 
   Size:        885     312
 
BclI T'GATC_A

Cuts at:      0     933    1197 
   Size:        933     264
 
BmgBI CAC'GTC

Cuts at:      0     336    1197 
   Size:        336     861
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     132     420     888    1197 
   Size:        132     288     468     309

  Fragments arranged by size: 

                 468     309     288     132
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     239    1197 
   Size:        239     958
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     581    1197 
   Size:        581     616
 
Bpu10I CC'TnA_GC

Cuts at:      0     884    1197 
   Size:        884     313
 
BsaAI yAC'GTr

Cuts at:      0     504    1197 
   Size:        504     693
 
BsaWI w'CCGG_w

Cuts at:      0     402    1197 
   Size:        402     795
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     859    1197 
   Size:        859     338
 
Bsp1286I G_dGCh'C

Cuts at:      0     885    1197 
   Size:        885     312
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     296    1197 
   Size:        296     901
 
BsrDI GCAATG_nn'

Cuts at:      0     719    1197 
   Size:        719     478
 
BstYI r'GATC_y

Cuts at:      0      43    1197 
   Size:         43    1154
 
Bsu36I CC'TnA_GG

Cuts at:      0    1145    1197 
   Size:       1145      52
 
EarI CTCTTCn'nnn_

Cuts at:      0     134     195    1197 
   Size:        134      61    1002

  Fragments arranged by size: 

                1002     134      61
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     107     402     581    1197 
   Size:        107     295     179     616

  Fragments arranged by size: 

                 616     295     179     107
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1132    1197 
   Size:       1132      65
 
HindIII A'AGCT_T

Cuts at:      0     911    1197 
   Size:        911     286
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1024    1197 
   Size:       1024     173
 
MslI CAynn'nnrTG

Cuts at:      0     730    1197 
   Size:        730     467
 
NspI r_CATG'y

Cuts at:      0      26    1197 
   Size:         26    1171
 
PasI CC'CwG_GG

Cuts at:      0     211    1197 
   Size:        211     986
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     275    1197 
   Size:        275     922
 
SphI G_CATG'C

Cuts at:      0      26    1197 
   Size:         26    1171
 
SspI AAT'ATT

Cuts at:      0     102    1186    1197 
   Size:        102    1084      11

  Fragments arranged by size: 

                1084     102      11
 
StyI C'CwwG_G

Cuts at:      0     174    1043    1128    1197 
   Size:        174     869      85      69

  Fragments arranged by size: 

                 869     174      85      69
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     634    1197 
   Size:        634     563
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     609     641    1197 
   Size:        609      32     556

  Fragments arranged by size: 

                 609     556      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     300    1007    1197 
   Size:        300     707     190

  Fragments arranged by size: 

                 707     300     190
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AlfI     AloI    AlwNI     ApoI     BanI
    BanII     BclI    BmgBI     BmrI     BplI     BpmI   Bpu10I    BsaAI
    BsaWI     BsgI Bsp1286I    BspMI    BsrDI    BstYI   Bsu36I     EarI
  Eco57MI    EcoNI  HindIII     MmeI     MslI     NspI     PasI    PflMI
     SphI     SspI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI Bme1580I     BmtI    BpuEI     BsaI    BsaBI
    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SrfI     StuI     SwaI     TatI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI