(Linear) (Six-base) MAPSORT of: KIEE0252.seq  Check: 8338  from: 1  to: 651



KIEE0252.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     651 
   Size:        151     500
 
AflIII A'CryG_T

Cuts at:      0     324     651 
   Size:        324     327
 
AleI CACnn'nnGTG

Cuts at:      0      93     651 
   Size:         93     558
 
AlwNI CAG_nnn'CTG

Cuts at:      0      18     651 
   Size:         18     633
 
BanI G'GyrC_C

Cuts at:      0      21      95     651 
   Size:         21      74     556

  Fragments arranged by size: 

                 556      74      21
 
Bme1580I G_kGCm'C

Cuts at:      0      26     100     651 
   Size:         26      74     551

  Fragments arranged by size: 

                 551      74      26
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     516     651 
   Size:        516     135
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     559     651 
   Size:        559      92
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     651 
   Size:         31     620
 
Bsp1286I G_dGCh'C

Cuts at:      0      26     100     651 
   Size:         26      74     551

  Fragments arranged by size: 

                 551      74      26
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     430     651 
   Size:        430     221
 
BsrDI GCAATG_nn'

Cuts at:      0     386     651 
   Size:        386     265
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     519     651 
   Size:        519     132
 
BstYI r'GATC_y

Cuts at:      0     353     411     651 
   Size:        353      58     240

  Fragments arranged by size: 

                 353     240      58
 
BtsI GCAGTG_nn'

Cuts at:      0     477     568     651 
   Size:        477      91      83

  Fragments arranged by size: 

                 477      91      83
 
EaeI y'GGCC_r

Cuts at:      0     261     651 
   Size:        261     390
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     559     651 
   Size:        151     408      92

  Fragments arranged by size: 

                 408     151      92
 
EcoO109I rG'GnC_Cy

Cuts at:      0      72     651 
   Size:         72     579
 
HaeII r_GCGC'y

Cuts at:      0      19     651 
   Size:         19     632
 
MslI CAynn'nnrTG

Cuts at:      0      93     623     651 
   Size:         93     530      28

  Fragments arranged by size: 

                 530      93      28
 
NspI r_CATG'y

Cuts at:      0     474     651 
   Size:        474     177
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     270     651 
   Size:        270     381
 
PfoI T'CCnGG_A

Cuts at:      0     349     651 
   Size:        349     302
 
PpuMI rG'GwC_Cy

Cuts at:      0      72     651 
   Size:         72     579
 
PsiI TTA'TAA

Cuts at:      0     163     651 
   Size:        163     488
 
SanDI GG'GwC_CC

Cuts at:      0      72     651 
   Size:         72     579
 
SfcI C'TryA_G

Cuts at:      0     171     651 
   Size:        171     480
 
SspI AAT'ATT

Cuts at:      0     556     651 
   Size:        556      95
 
StyI C'CwwG_G

Cuts at:      0     136     269     651 
   Size:        136     133     382

  Fragments arranged by size: 

                 382     136     133
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     236     241     651 
   Size:        236       5     410

  Fragments arranged by size: 

                 410     236       5
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      57     651 
   Size:         57     594
 

 Enzymes that do cut:

     AcuI   AflIII     AleI    AlwNI     BanI Bme1580I     BmrI     BpmI
     BsgI Bsp1286I    BspMI    BsrDI    BstXI    BstYI     BtsI     EaeI
  Eco57MI EcoO109I    HaeII     MslI     NspI    PflMI     PfoI    PpuMI
     PsiI    SanDI     SfcI     SspI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI