(Linear) (Six-base) MAPSORT of: KIEE0248.seq  Check: 953  from: 1  to: 393



KIEE0248.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AflIII A'CryG_T

Cuts at:      0     164     393 
   Size:        164     229
 
AlwNI CAG_nnn'CTG

Cuts at:      0      15     393 
   Size:         15     378
 
BanII G_rGCy'C

Cuts at:      0      62     393 
   Size:         62     331
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     244     258     393 
   Size:        244      14     135

  Fragments arranged by size: 

                 244     135      14
 
BmgBI CAC'GTC

Cuts at:      0     339     393 
   Size:        339      54
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     298     393 
   Size:        298      95
 
BmtI G_CTAG'C

Cuts at:      0     208     393 
   Size:        208     185
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      70     393 
   Size:         70     323
 
BsaAI yAC'GTr

Cuts at:      0     165     393 
   Size:        165     228
 
BsaHI Gr'CG_yC

Cuts at:      0     113     393 
   Size:        113     280
 
BsaWI w'CCGG_w

Cuts at:      0     135     297     393 
   Size:        135     162      96

  Fragments arranged by size: 

                 162     135      96
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      22      52     208     238     393 
   Size:         22      30     156      30     155

  Fragments arranged by size: 

                 156     155      30      30      22
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      88     213     364     393 
   Size:         88     125     151      29

  Fragments arranged by size: 

                 151     125      88      29
 
BsiHKAI G_wGCw'C

Cuts at:      0      43      62     393 
   Size:         43      19     331

  Fragments arranged by size: 

                 331      43      19
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     120     393 
   Size:        120     273
 
Bsp1286I G_dGCh'C

Cuts at:      0      43      62     393 
   Size:         43      19     331

  Fragments arranged by size: 

                 331      43      19
 
BspEI T'CCGG_A

Cuts at:      0     135     297     393 
   Size:        135     162      96

  Fragments arranged by size: 

                 162     135      96
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      48     393 
   Size:         48     345
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     112     393 
   Size:        112     281
 
EaeI y'GGCC_r

Cuts at:      0      17     393 
   Size:         17     376
 
EarI CTCTTCn'nnn_

Cuts at:      0     317     341     393 
   Size:        317      24      52

  Fragments arranged by size: 

                 317      52      24
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     376     393 
   Size:        376      17
 
EcoICRI GAG'CTC

Cuts at:      0      60     393 
   Size:         60     333
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     205     237     393 
   Size:        205      32     156

  Fragments arranged by size: 

                 205     156      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      68     393 
   Size:         68     325
 
MspA1I CmG'CkG

Cuts at:      0      15     393 
   Size:         15     378
 
NheI G'CTAG_C

Cuts at:      0     204     393 
   Size:        204     189
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      15     393 
   Size:         15     378
 
PfoI T'CCnGG_A

Cuts at:      0     254     393 
   Size:        254     139
 
PmlI CAC'GTG

Cuts at:      0     165     393 
   Size:        165     228
 
PvuII CAG'CTG

Cuts at:      0      15     393 
   Size:         15     378
 
SacI G_AGCT'C

Cuts at:      0      62     393 
   Size:         62     331
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      94     393 
   Size:         94     299
 
TatI w'GTAC_w

Cuts at:      0     326     393 
   Size:        326      67
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      22      54     393 
   Size:         22      32     339

  Fragments arranged by size: 

                 339      32      22
 

 Enzymes that do cut:

   AflIII    AlwNI    BanII     BbsI    BmgBI     BmrI     BmtI     BsaI
    BsaAI    BsaHI    BsaWI    BsaXI    BseRI  BsiHKAI    BsmBI Bsp1286I
    BspEI    BtgZI     DrdI     EaeI     EarI     EciI  EcoICRI    Hin4I
     MmeI   MspA1I     NheI    PflMI     PfoI     PmlI    PvuII     SacI
    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     EagI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI