Restriction map for
pF1KE0239
(Linear) (Six-base) MAPSORT of: KIEE0239.seq Check: 5560 from: 1 to: 825
KIEE0239.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 596 825
Size: 349 247 229
Fragments arranged by size:
349 247 229
AleI CACnn'nnGTG
Cuts at: 0 462 825
Size: 462 363
ApaLI G'TGCA_C
Cuts at: 0 121 331 825
Size: 121 210 494
Fragments arranged by size:
494 210 121
ApoI r'AATT_y
Cuts at: 0 514 825
Size: 514 311
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 14 47 825
Size: 14 33 778
Fragments arranged by size:
778 33 14
BanI G'GyrC_C
Cuts at: 0 658 825
Size: 658 167
BanII G_rGCy'C
Cuts at: 0 23 825
Size: 23 802
BbsI GAAGACnn'nnnn_
Cuts at: 0 355 825
Size: 355 470
BbvCI CC'TCA_GC
Cuts at: 0 382 825
Size: 382 443
BlpI GC'TnA_GC
Cuts at: 0 244 825
Size: 244 581
Bme1580I G_kGCm'C
Cuts at: 0 125 335 663 825
Size: 125 210 328 162
Fragments arranged by size:
328 210 162 125
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 825
Size: 167 658
Bpu10I CC'TnA_GC
Cuts at: 0 382 825
Size: 382 443
BsaI GGTCTCn'nnnn_
Cuts at: 0 101 825
Size: 101 724
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 810 813 825
Size: 810 3 12
Fragments arranged by size:
810 12 3
BseYI C'CCAG_C
Cuts at: 0 168 825
Size: 168 657
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 825
Size: 255 570
BsiHKAI G_wGCw'C
Cuts at: 0 23 125 335 501 825
Size: 23 102 210 166 324
Fragments arranged by size:
324 210 166 102 23
BsmI GAATG_Cn'
Cuts at: 0 248 825
Size: 248 577
Bsp1286I G_dGCh'C
Cuts at: 0 23 125 335 501 663 825
Size: 23 102 210 166 162 162
Fragments arranged by size:
210 166 162 162 102 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 277 825
Size: 277 548
Bsu36I CC'TnA_GG
Cuts at: 0 72 825
Size: 72 753
BtgI C'CryG_G
Cuts at: 0 339 825
Size: 339 486
BtsI GCAGTG_nn'
Cuts at: 0 23 272 333 825
Size: 23 249 61 492
Fragments arranged by size:
492 249 61 23
EarI CTCTTCn'nnn_
Cuts at: 0 76 416 804 825
Size: 76 340 388 21
Fragments arranged by size:
388 340 76 21
EcoICRI GAG'CTC
Cuts at: 0 21 825
Size: 21 804
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 349 596 825
Size: 167 182 247 229
Fragments arranged by size:
247 229 182 167
EcoNI CCTnn'n_nnAGG
Cuts at: 0 71 825
Size: 71 754
EcoRI G'AATT_C
Cuts at: 0 514 825
Size: 514 311
EcoRV GAT'ATC
Cuts at: 0 291 825
Size: 291 534
MslI CAynn'nnrTG
Cuts at: 0 416 462 530 825
Size: 416 46 68 295
Fragments arranged by size:
416 295 68 46
PsiI TTA'TAA
Cuts at: 0 416 592 825
Size: 416 176 233
Fragments arranged by size:
416 233 176
PstI C_TGCA'G
Cuts at: 0 30 825
Size: 30 795
SacI G_AGCT'C
Cuts at: 0 23 825
Size: 23 802
SapI GCTCTTCn'nnn_
Cuts at: 0 804 825
Size: 804 21
SfcI C'TryA_G
Cuts at: 0 26 96 555 725 825
Size: 26 70 459 170 100
Fragments arranged by size:
459 170 100 70 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 170 825
Size: 170 655
Enzymes that do cut:
AcuI AleI ApaLI ApoI BaeI BanI BanII BbsI
BbvCI BlpI Bme1580I BpmI Bpu10I BsaI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspMI Bsu36I BtgI BtsI
EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV MslI PsiI
PstI SacI SapI SfcI TaqII
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BamHI BbeI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoO109I
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI