(Linear) (Six-base) MAPSORT of: KIEE0238.seq  Check: 7551  from: 1  to: 486



KIEE0238.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

BamHI G'GATC_C

Cuts at:      0     452     486 
   Size:        452      34
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      78     486 
   Size:         78     408
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     486 
   Size:        149     337
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      26     486 
   Size:         26     460
 
BsaAI yAC'GTr

Cuts at:      0      81     486 
   Size:         81     405
 
BsaHI Gr'CG_yC

Cuts at:      0     368     486 
   Size:        368     118
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     304     486 
   Size:        304     182
 
BseYI C'CCAG_C

Cuts at:      0     205     486 
   Size:        205     281
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     416     486 
   Size:        416      70
 
BsiHKAI G_wGCw'C

Cuts at:      0     344     486 
   Size:        344     142
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     375     486 
   Size:        375     111
 
Bsp1286I G_dGCh'C

Cuts at:      0     344     486 
   Size:        344     142
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     309     486 
   Size:        309     177
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      81     391     486 
   Size:         81     310      95

  Fragments arranged by size: 

                 310      95      81
 
BstYI r'GATC_y

Cuts at:      0     452     486 
   Size:        452      34
 
BtsI GCAGTG_nn'

Cuts at:      0     256     384     486 
   Size:        256     128     102

  Fragments arranged by size: 

                 256     128     102
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     428     463     486 
   Size:        428      35      23

  Fragments arranged by size: 

                 428      35      23
 
DraIII CAC_nnn'GTG

Cuts at:      0     348     486 
   Size:        348     138
 
EaeI y'GGCC_r

Cuts at:      0       8     140     461     486 
   Size:          8     132     321      25

  Fragments arranged by size: 

                 321     132      25       8
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     149     486 
   Size:        149     337
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     290     486 
   Size:        290     196
 
MscI TGG'CCA

Cuts at:      0      10     463     486 
   Size:         10     453      23

  Fragments arranged by size: 

                 453      23      10
 
MslI CAynn'nnrTG

Cuts at:      0      79     486 
   Size:         79     407
 
SexAI A'CCwGG_T

Cuts at:      0     383     486 
   Size:        383     103
 
SfcI C'TryA_G

Cuts at:      0     219     486 
   Size:        219     267
 
StuI AGG'CCT

Cuts at:      0     425     486 
   Size:        425      61
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      36     486 
   Size:         36     450
 
Tth111I GACn'n_nGTC

Cuts at:      0     385     486 
   Size:        385     101
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     361     486 
   Size:        361     125
 

 Enzymes that do cut:

    BamHI     BmrI     BpmI     BsaI    BsaAI    BsaHI    BseRI    BseYI
     BsgI  BsiHKAI    BsmBI Bsp1286I   BstAPI    BstXI    BstYI     BtsI
    CspCI   DraIII     EaeI  Eco57MI     MmeI     MscI     MslI    SexAI
     SfcI     StuI    TaqII  Tth111I     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI
   Bpu10I    BpuEI    BsaBI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
     DraI     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TatI     TstI     XbaI     XhoI     XmaI     XmnI     ZraI