(Linear) (Six-base) MAPSORT of: KIEE0235.seq  Check: 7649  from: 1  to: 939



KIEE0235.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AflIII A'CryG_T

Cuts at:      0     921     939 
   Size:        921      18
 
ApaI G_GGCC'C

Cuts at:      0     784     939 
   Size:        784     155
 
ApoI r'AATT_y

Cuts at:      0     192     939 
   Size:        192     747
 
AseI AT'TA_AT

Cuts at:      0     433     581     939 
   Size:        433     148     358

  Fragments arranged by size: 

                 433     358     148
 
BanII G_rGCy'C

Cuts at:      0     729     784     939 
   Size:        729      55     155

  Fragments arranged by size: 

                 729     155      55
 
BclI T'GATC_A

Cuts at:      0     804     939 
   Size:        804     135
 
Bme1580I G_kGCm'C

Cuts at:      0     784     939 
   Size:        784     155
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     748     939 
   Size:        748     191
 
BsmI GAATG_Cn'

Cuts at:      0      88     292     939 
   Size:         88     204     647

  Fragments arranged by size: 

                 647     204      88
 
Bsp1286I G_dGCh'C

Cuts at:      0     729     784     939 
   Size:        729      55     155

  Fragments arranged by size: 

                 729     155      55
 
BsrDI GCAATG_nn'

Cuts at:      0      19     939 
   Size:         19     920
 
BtgI C'CryG_G

Cuts at:      0     326     939 
   Size:        326     613
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      46      81     939 
   Size:         46      35     858

  Fragments arranged by size: 

                 858      46      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     670     739     772     939 
   Size:        670      69      33     167

  Fragments arranged by size: 

                 670     167      69      33
 
EcoO109I rG'GnC_Cy

Cuts at:      0     781     939 
   Size:        781     158
 
EcoRV GAT'ATC

Cuts at:      0     218     275     939 
   Size:        218      57     664

  Fragments arranged by size: 

                 664     218      57
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     261     293     407     439     749     781     939 
   Size:        261      32     114      32     310      32     158

  Fragments arranged by size: 

                 310     261     158     114      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     536     939 
   Size:        536     403
 
MslI CAynn'nnrTG

Cuts at:      0     115     775     907     939 
   Size:        115     660     132      32

  Fragments arranged by size: 

                 660     132     115      32
 
NdeI CA'TA_TG

Cuts at:      0     357     376     444     939 
   Size:        357      19      68     495

  Fragments arranged by size: 

                 495     357      68      19
 
NspI r_CATG'y

Cuts at:      0     925     939 
   Size:        925      14
 
PciI A'CATG_T

Cuts at:      0     921     939 
   Size:        921      18
 
PspOMI G'GGCC_C

Cuts at:      0     780     939 
   Size:        780     159
 
SfcI C'TryA_G

Cuts at:      0     867     939 
   Size:        867      72
 
StyI C'CwwG_G

Cuts at:      0     186     939 
   Size:        186     753
 
TatI w'GTAC_w

Cuts at:      0     176     519     565     939 
   Size:        176     343      46     374

  Fragments arranged by size: 

                 374     343     176      46
 
XmnI GAAnn'nnTTC

Cuts at:      0     250     335     939 
   Size:        250      85     604

  Fragments arranged by size: 

                 604     250      85
 

 Enzymes that do cut:

   AflIII     ApaI     ApoI     AseI    BanII     BclI Bme1580I     BplI
     BsmI Bsp1286I    BsrDI     BtgI    CspCI     EarI EcoO109I    EcoRV
    Hin4I     MmeI     MslI     NdeI     NspI     PciI   PspOMI     SfcI
     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmrI     BmtI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI     DraI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI     FalI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI