(Linear) (Six-base) MAPSORT of: KIEE0228.seq  Check: 4751  from: 1  to: 330



KIEE0228.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     330 
   Size:         37     293
 
ApoI r'AATT_y

Cuts at:      0     209     330 
   Size:        209     121
 
AvaI C'yCGr_G

Cuts at:      0     161     330 
   Size:        161     169
 
BanII G_rGCy'C

Cuts at:      0     198     330 
   Size:        198     132
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     242     330 
   Size:        242      88
 
BlpI GC'TnA_GC

Cuts at:      0     103     330 
   Size:        103     227
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      85     330 
   Size:         85     245
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     330 
   Size:        182     148
 
BseYI C'CCAG_C

Cuts at:      0     258     330 
   Size:        258      72
 
Bsp1286I G_dGCh'C

Cuts at:      0     198     330 
   Size:        198     132
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     262     330 
   Size:        262      68
 
EaeI y'GGCC_r

Cuts at:      0       2     330 
   Size:          2     328
 
EarI CTCTTCn'nnn_

Cuts at:      0      19     107     330 
   Size:         19      88     223

  Fragments arranged by size: 

                 223      88      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     182     330 
   Size:         37     145     148

  Fragments arranged by size: 

                 148     145      37
 
EcoO109I rG'GnC_Cy

Cuts at:      0      12     330 
   Size:         12     318
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     301     330 
   Size:        301      29
 
NspI r_CATG'y

Cuts at:      0     206     330 
   Size:        206     124
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     283     330 
   Size:        283      47
 
PpuMI rG'GwC_Cy

Cuts at:      0      12     330 
   Size:         12     318
 
ScaI AGT'ACT

Cuts at:      0     320     330 
   Size:        320      10
 
SfcI C'TryA_G

Cuts at:      0     143     330 
   Size:        143     187
 
SphI G_CATG'C

Cuts at:      0     206     330 
   Size:        206     124
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      67     330 
   Size:         67     263
 
TatI w'GTAC_w

Cuts at:      0     318     330 
   Size:        318      12
 
Tth111I GACn'n_nGTC

Cuts at:      0      10     330 
   Size:         10     320
 

 Enzymes that do cut:

     AcuI     ApoI     AvaI    BanII     BbsI     BlpI     BmrI     BpmI
    BseYI Bsp1286I    BspMI     EaeI     EarI  Eco57MI EcoO109I     MmeI
     NspI    PflMI    PpuMI     ScaI     SfcI     SphI    TaqII     TatI
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI
     SspI     StuI     StyI     SwaI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI