(Linear) (Six-base) MAPSORT of: KIEE0214.seq  Check: 3473  from: 1  to: 1383



KIEE0214.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:      0     259    1383 
   Size:        259    1124
 
Acc65I G'GTAC_C

Cuts at:      0    1144    1238    1383 
   Size:       1144      94     145

  Fragments arranged by size: 

                1144     145      94
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1358    1383 
   Size:       1358      25
 
AgeI A'CCGG_T

Cuts at:      0      58    1383 
   Size:         58    1325
 
AleI CACnn'nnGTG

Cuts at:      0    1084    1383 
   Size:       1084     299
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1228    1383 
   Size:       1228     155
 
ApoI r'AATT_y

Cuts at:      0     301    1214    1383 
   Size:        301     913     169

  Fragments arranged by size: 

                 913     301     169
 
AseI AT'TA_AT

Cuts at:      0     241    1383 
   Size:        241    1142
 
AvrII C'CTAG_G

Cuts at:      0     936    1383 
   Size:        936     447
 
BanI G'GyrC_C

Cuts at:      0    1065    1144    1238    1383 
   Size:       1065      79      94     145

  Fragments arranged by size: 

                1065     145      94      79
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     311    1383 
   Size:        311    1072
 
BclI T'GATC_A

Cuts at:      0     285     468    1383 
   Size:        285     183     915

  Fragments arranged by size: 

                 915     285     183
 
BsaWI w'CCGG_w

Cuts at:      0      58    1383 
   Size:         58    1325
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     647     677    1383 
   Size:        647      30     706

  Fragments arranged by size: 

                 706     647      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1199    1383 
   Size:       1199     184
 
BsiHKAI G_wGCw'C

Cuts at:      0     333    1383 
   Size:        333    1050
 
BsmI GAATG_Cn'

Cuts at:      0     178     756    1383 
   Size:        178     578     627

  Fragments arranged by size: 

                 627     578     178
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     759    1383 
   Size:        759     624
 
Bsp1286I G_dGCh'C

Cuts at:      0     333    1383 
   Size:        333    1050
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      30    1383 
   Size:         30    1353
 
BsrDI GCAATG_nn'

Cuts at:      0     827    1383 
   Size:        827     556
 
BsrFI r'CCGG_y

Cuts at:      0      58    1383 
   Size:         58    1325
 
BstBI TT'CG_AA

Cuts at:      0     501    1383 
   Size:        501     882
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     872    1383 
   Size:        872     511
 
BstYI r'GATC_y

Cuts at:      0     657    1383 
   Size:        657     726
 
BtsI GCAGTG_nn'

Cuts at:      0    1190    1383 
   Size:       1190     193
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1358    1383 
   Size:       1358      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     661     693     761     793    1383 
   Size:        661      32      68      32     590

  Fragments arranged by size: 

                 661     590      68      32      32
 
KpnI G_GTAC'C

Cuts at:      0    1148    1242    1383 
   Size:       1148      94     141

  Fragments arranged by size: 

                1148     141      94
 
MslI CAynn'nnrTG

Cuts at:      0    1084    1383 
   Size:       1084     299
 
MspA1I CmG'CkG

Cuts at:      0    1319    1383 
   Size:       1319      64
 
NdeI CA'TA_TG

Cuts at:      0     977    1383 
   Size:        977     406
 
NspI r_CATG'y

Cuts at:      0     741    1383 
   Size:        741     642
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     347     440    1383 
   Size:        347      93     943

  Fragments arranged by size: 

                 943     347      93
 
PfoI T'CCnGG_A

Cuts at:      0     182    1383 
   Size:        182    1201
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1331    1363    1383 
   Size:       1331      32      20

  Fragments arranged by size: 

                1331      32      20
 
PstI C_TGCA'G

Cuts at:      0     224    1197    1383 
   Size:        224     973     186

  Fragments arranged by size: 

                 973     224     186
 
PvuII CAG'CTG

Cuts at:      0    1319    1383 
   Size:       1319      64
 
SfcI C'TryA_G

Cuts at:      0     220    1193    1383 
   Size:        220     973     190

  Fragments arranged by size: 

                 973     220     190
 
SpeI A'CTAG_T

Cuts at:      0     928    1383 
   Size:        928     455
 
SspI AAT'ATT

Cuts at:      0     138    1383 
   Size:        138    1245
 
StyI C'CwwG_G

Cuts at:      0     936    1383 
   Size:        936     447
 
TatI w'GTAC_w

Cuts at:      0    1036    1383 
   Size:       1036     347
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     647     679    1383 
   Size:        647      32     704

  Fragments arranged by size: 

                 704     647      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     971    1383 
   Size:        971     412
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AgeI     AleI    AlwNI     ApoI     AseI
    AvrII     BanI     BbsI     BclI    BsaWI    BsaXI    BseRI  BsiHKAI
     BsmI    BsmBI Bsp1286I    BspMI    BsrDI    BsrFI    BstBI    BstXI
    BstYI     BtsI  Eco57MI    Hin4I     KpnI     MslI   MspA1I     NdeI
     NspI    PflMI     PfoI     PsrI     PstI    PvuII     SfcI     SpeI
     SspI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AhdI     AlfI
     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI     BaeI    BamHI
    BanII     BbeI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseYI     BsgI    BsiEI
    BsiWI    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI   BstAPI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI     StuI     SwaI
    TaqII  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI