Restriction map for
pF1KE0209
(Linear) (Six-base) MAPSORT of: KIEE0209.seq Check: 5822 from: 1 to: 909
KIEE0209.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AfeI AGC'GCT
Cuts at: 0 297 909
Size: 297 612
ApaI G_GGCC'C
Cuts at: 0 282 909
Size: 282 627
ApoI r'AATT_y
Cuts at: 0 523 780 909
Size: 523 257 129
Fragments arranged by size:
523 257 129
BanI G'GyrC_C
Cuts at: 0 853 909
Size: 853 56
BanII G_rGCy'C
Cuts at: 0 282 909
Size: 282 627
BbeI G_GCGC'C
Cuts at: 0 857 909
Size: 857 52
BbvCI CC'TCA_GC
Cuts at: 0 817 909
Size: 817 92
BglI GCCn_nnn'nGGC
Cuts at: 0 21 909
Size: 21 888
Bme1580I G_kGCm'C
Cuts at: 0 282 909
Size: 282 627
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 909
Size: 582 327
Bpu10I CC'TnA_GC
Cuts at: 0 817 909
Size: 817 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 448 909
Size: 448 461
BsaBI GATnn'nnATC
Cuts at: 0 316 909
Size: 316 593
BsaHI Gr'CG_yC
Cuts at: 0 854 909
Size: 854 55
BsaWI w'CCGG_w
Cuts at: 0 545 676 776 909
Size: 545 131 100 133
Fragments arranged by size:
545 133 131 100
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 253 283 373 403 565 595 909
Size: 253 30 90 30 162 30 314
Fragments arranged by size:
314 253 162 90 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 892 909
Size: 892 17
BseYI C'CCAG_C
Cuts at: 0 132 174 194 275 736 909
Size: 132 42 20 81 461 173
Fragments arranged by size:
461 173 132 81 42 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 909
Size: 379 530
BsiEI CG_ry'CG
Cuts at: 0 28 909
Size: 28 881
BsiHKAI G_wGCw'C
Cuts at: 0 884 909
Size: 884 25
Bsp1286I G_dGCh'C
Cuts at: 0 282 884 909
Size: 282 602 25
Fragments arranged by size:
602 282 25
BspEI T'CCGG_A
Cuts at: 0 545 776 909
Size: 545 231 133
Fragments arranged by size:
545 231 133
BspHI T'CATG_A
Cuts at: 0 159 909
Size: 159 750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 103 466 909
Size: 103 363 443
Fragments arranged by size:
443 363 103
BsrDI GCAATG_nn'
Cuts at: 0 213 525 909
Size: 213 312 384
Fragments arranged by size:
384 312 213
BsrFI r'CCGG_y
Cuts at: 0 101 909
Size: 101 808
BsrGI T'GTAC_A
Cuts at: 0 886 909
Size: 886 23
BssSI C'ACGA_G
Cuts at: 0 40 909
Size: 40 869
BstXI CCAn_nnnn'nTGG
Cuts at: 0 300 909
Size: 300 609
BstYI r'GATC_y
Cuts at: 0 180 909
Size: 180 729
BtgI C'CryG_G
Cuts at: 0 796 909
Size: 796 113
EaeI y'GGCC_r
Cuts at: 0 25 909
Size: 25 884
EagI C'GGCC_G
Cuts at: 0 25 909
Size: 25 884
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 70 319 909
Size: 70 249 590
Fragments arranged by size:
590 249 70
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 909
Size: 582 327
EcoO109I rG'GnC_Cy
Cuts at: 0 897 909
Size: 897 12
EcoRI G'AATT_C
Cuts at: 0 523 780 909
Size: 523 257 129
Fragments arranged by size:
523 257 129
HaeII r_GCGC'y
Cuts at: 0 299 857 909
Size: 299 558 52
Fragments arranged by size:
558 299 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 154 186 565 597 909
Size: 154 32 379 32 312
Fragments arranged by size:
379 312 154 32 32
HincII GTy'rAC
Cuts at: 0 222 909
Size: 222 687
KasI G'GCGC_C
Cuts at: 0 853 909
Size: 853 56
MspA1I CmG'CkG
Cuts at: 0 132 178 638 909
Size: 132 46 460 271
Fragments arranged by size:
460 271 132 46
NarI GG'CG_CC
Cuts at: 0 854 909
Size: 854 55
NcoI C'CATG_G
Cuts at: 0 796 909
Size: 796 113
NotI GC'GGCC_GC
Cuts at: 0 25 909
Size: 25 884
NspI r_CATG'y
Cuts at: 0 518 909
Size: 518 391
PflMI CCAn_nnn'nTGG
Cuts at: 0 194 909
Size: 194 715
PpuMI rG'GwC_Cy
Cuts at: 0 897 909
Size: 897 12
PspOMI G'GGCC_C
Cuts at: 0 278 909
Size: 278 631
PvuII CAG'CTG
Cuts at: 0 638 909
Size: 638 271
SanDI GG'GwC_CC
Cuts at: 0 897 909
Size: 897 12
ScaI AGT'ACT
Cuts at: 0 766 909
Size: 766 143
SfoI GGC'GCC
Cuts at: 0 855 909
Size: 855 54
StyI C'CwwG_G
Cuts at: 0 476 796 909
Size: 476 320 113
Fragments arranged by size:
476 320 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 728 909
Size: 728 181
TatI w'GTAC_w
Cuts at: 0 764 886 909
Size: 764 122 23
Fragments arranged by size:
764 122 23
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 472 504 909
Size: 472 32 405
Fragments arranged by size:
472 405 32
Enzymes that do cut:
AfeI ApaI ApoI BanI BanII BbeI BbvCI BglI
Bme1580I BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstXI BstYI BtgI
EaeI EagI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I
HincII KasI MspA1I NarI NcoI NotI NspI PflMI
PpuMI PspOMI PvuII SanDI ScaI SfoI StyI TaqII
TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbsI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI
BplI BpuEI BsaAI BsiWI BsmI BsmBI BsrBI BssHII
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRV
FalI FseI FspI FspAI HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI NaeI NdeI NgoMIV NheI
NruI NsiI PacI PasI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SapI SbfI SexAI SfcI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI