(Linear) (Six-base) MAPSORT of: KIEE0196.seq  Check: 83  from: 1  to: 1248



KIEE0196.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     641    1248 
   Size:        641     607
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     851     896    1248 
   Size:        851      45     352

  Fragments arranged by size: 

                 851     352      45
 
AleI CACnn'nnGTG

Cuts at:      0     626    1248 
   Size:        626     622
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     690    1248 
   Size:        690     558
 
AlwNI CAG_nnn'CTG

Cuts at:      0     448    1248 
   Size:        448     800
 
ApoI r'AATT_y

Cuts at:      0     258     346     998    1248 
   Size:        258      88     652     250

  Fragments arranged by size: 

                 652     258     250      88
 
AvaI C'yCGr_G

Cuts at:      0    1202    1248 
   Size:       1202      46
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      91     133     862    1248 
   Size:         91      42     729     386

  Fragments arranged by size: 

                 729     386      91      42
 
BclI T'GATC_A

Cuts at:      0     641    1248 
   Size:        641     607
 
BfrBI ATG'CAT

Cuts at:      0     250    1018    1081    1248 
   Size:        250     768      63     167

  Fragments arranged by size: 

                 768     250     167      63
 
BglII A'GATC_T

Cuts at:      0      28    1248 
   Size:         28    1220
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     344     595    1248 
   Size:        182     162     251     653

  Fragments arranged by size: 

                 653     251     182     162
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     713     743    1248 
   Size:        713      30     505

  Fragments arranged by size: 

                 713     505      30
 
BseYI C'CCAG_C

Cuts at:      0    1157    1248 
   Size:       1157      91
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     352     841    1248 
   Size:        352     489     407

  Fragments arranged by size: 

                 489     407     352
 
BsmI GAATG_Cn'

Cuts at:      0     444    1248 
   Size:        444     804
 
BspHI T'CATG_A

Cuts at:      0     774     801    1248 
   Size:        774      27     447

  Fragments arranged by size: 

                 774     447      27
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     641    1248 
   Size:        641     607
 
BsrDI GCAATG_nn'

Cuts at:      0     613    1011    1248 
   Size:        613     398     237

  Fragments arranged by size: 

                 613     398     237
 
BsrGI T'GTAC_A

Cuts at:      0       2    1248 
   Size:          2    1246
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     448    1248 
   Size:        448     800
 
BstYI r'GATC_y

Cuts at:      0      28    1248 
   Size:         28    1220
 
BtgI C'CryG_G

Cuts at:      0     589    1248 
   Size:        589     659
 
BtsI GCAGTG_nn'

Cuts at:      0     672    1248 
   Size:        672     576
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     492     527    1248 
   Size:        492      35     721

  Fragments arranged by size: 

                 721     492      35
 
EaeI y'GGCC_r

Cuts at:      0      34     586    1248 
   Size:         34     552     662

  Fragments arranged by size: 

                 662     552      34
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     344     595     851     896    1248 
   Size:        182     162     251     256      45     352

  Fragments arranged by size: 

                 352     256     251     182     162      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1200    1232    1248 
   Size:       1200      32      16

  Fragments arranged by size: 

                1200      32      16
 
HincII GTy'rAC

Cuts at:      0     714    1248 
   Size:        714     534
 
MfeI C'AATT_G

Cuts at:      0    1221    1248 
   Size:       1221      27
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1125    1248 
   Size:       1125     123
 
MscI TGG'CCA

Cuts at:      0      36     588    1248 
   Size:         36     552     660

  Fragments arranged by size: 

                 660     552      36
 
MslI CAynn'nnrTG

Cuts at:      0     588     626    1248 
   Size:        588      38     622

  Fragments arranged by size: 

                 622     588      38
 
MspA1I CmG'CkG

Cuts at:      0     342    1248 
   Size:        342     906
 
NcoI C'CATG_G

Cuts at:      0     589    1248 
   Size:        589     659
 
NdeI CA'TA_TG

Cuts at:      0    1014    1248 
   Size:       1014     234
 
NsiI A_TGCA'T

Cuts at:      0     252    1020    1083    1248 
   Size:        252     768      63     165

  Fragments arranged by size: 

                 768     252     165      63
 
NspI r_CATG'y

Cuts at:      0     606    1085    1248 
   Size:        606     479     163

  Fragments arranged by size: 

                 606     479     163
 
PfoI T'CCnGG_A

Cuts at:      0     433    1248 
   Size:        433     815
 
PspXI vC'TCGA_Gb

Cuts at:      0    1202    1248 
   Size:       1202      46
 
PstI C_TGCA'G

Cuts at:      0     669    1248 
   Size:        669     579
 
PvuII CAG'CTG

Cuts at:      0     342    1248 
   Size:        342     906
 
SfcI C'TryA_G

Cuts at:      0     665    1248 
   Size:        665     583
 
SmlI C'TyrA_G

Cuts at:      0    1202    1248 
   Size:       1202      46
 
SphI G_CATG'C

Cuts at:      0     606    1248 
   Size:        606     642
 
StyI C'CwwG_G

Cuts at:      0     589    1248 
   Size:        589     659
 
TatI w'GTAC_w

Cuts at:      0       2     851    1248 
   Size:          2     849     397

  Fragments arranged by size: 

                 849     397       2
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     740     772    1248 
   Size:        740      32     476

  Fragments arranged by size: 

                 740     476      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     462    1248 
   Size:        462     786
 
XhoI C'TCGA_G

Cuts at:      0    1202    1248 
   Size:       1202      46
 

 Enzymes that do cut:

     AarI     AcuI     AleI     AlfI    AlwNI     ApoI     AvaI     BbsI
     BclI    BfrBI    BglII     BpmI    BsaXI    BseYI     BsgI     BsmI
    BspHI    BspMI    BsrDI    BsrGI   BstAPI    BstYI     BtgI     BtsI
    CspCI     EaeI  Eco57MI    Hin4I   HincII     MfeI     MmeI     MscI
     MslI   MspA1I     NcoI     NdeI     NsiI     NspI     PfoI    PspXI
     PstI    PvuII     SfcI     SmlI     SphI     StyI     TatI     TstI
     XcmI     XhoI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BsiEI
  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI   BssHII
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MluI     NaeI     NarI   NgoMIV
     NheI     NotI     NruI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XmaI
     XmnI     ZraI