(Linear) (Six-base) MAPSORT of: KIEE0187.seq  Check: 1551  from: 1  to: 732



KIEE0187.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:      0     583     732 
   Size:        583     149
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     233     732 
   Size:        233     499
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     423     455     732 
   Size:        423      32     277

  Fragments arranged by size: 

                 423     277      32
 
ApaI G_GGCC'C

Cuts at:      0      16     732 
   Size:         16     716
 
ApoI r'AATT_y

Cuts at:      0     652     732 
   Size:        652      80
 
BanII G_rGCy'C

Cuts at:      0      16     732 
   Size:         16     716
 
BbvCI CC'TCA_GC

Cuts at:      0      63     732 
   Size:         63     669
 
BglII A'GATC_T

Cuts at:      0      78     732 
   Size:         78     654
 
Bme1580I G_kGCm'C

Cuts at:      0      16     732 
   Size:         16     716
 
Bpu10I CC'TnA_GC

Cuts at:      0      63     732 
   Size:         63     669
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     414     732 
   Size:        414     318
 
BsaBI GATnn'nnATC

Cuts at:      0     206     732 
   Size:        206     526
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      51     732 
   Size:         51     681
 
Bsp1286I G_dGCh'C

Cuts at:      0      16     732 
   Size:         16     716
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     502     732 
   Size:        502     230
 
BstYI r'GATC_y

Cuts at:      0      78     732 
   Size:         78     654
 
EaeI y'GGCC_r

Cuts at:      0     461     492     732 
   Size:        461      31     240

  Fragments arranged by size: 

                 461     240      31
 
EarI CTCTTCn'nnn_

Cuts at:      0     677     732 
   Size:        677      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     233     732 
   Size:        233     499
 
EcoO109I rG'GnC_Cy

Cuts at:      0     349     732 
   Size:        349     383
 
EcoRI G'AATT_C

Cuts at:      0     652     732 
   Size:        652      80
 
EcoRV GAT'ATC

Cuts at:      0     383     732 
   Size:        383     349
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     246     732 
   Size:        246     486
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     119     151     318     350     732 
   Size:        119      32     167      32     382

  Fragments arranged by size: 

                 382     167     119      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     543     732 
   Size:        543     189
 
MscI TGG'CCA

Cuts at:      0     463     494     732 
   Size:        463      31     238

  Fragments arranged by size: 

                 463     238      31
 
NdeI CA'TA_TG

Cuts at:      0     489     732 
   Size:        489     243
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     304     732 
   Size:        304     428
 
PpuMI rG'GwC_Cy

Cuts at:      0     349     732 
   Size:        349     383
 
PsiI TTA'TAA

Cuts at:      0     421     592     732 
   Size:        421     171     140

  Fragments arranged by size: 

                 421     171     140
 
PspOMI G'GGCC_C

Cuts at:      0      12     732 
   Size:         12     720
 
SfcI C'TryA_G

Cuts at:      0     146     621     732 
   Size:        146     475     111

  Fragments arranged by size: 

                 475     146     111
 
StyI C'CwwG_G

Cuts at:      0     501     732 
   Size:        501     231
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     634     732 
   Size:        634      98
 
TatI w'GTAC_w

Cuts at:      0     549     732 
   Size:        549     183
 

 Enzymes that do cut:

     AccI     AcuI     AloI     ApaI     ApoI    BanII    BbvCI    BglII
 Bme1580I   Bpu10I     BsaI    BsaBI    BseRI Bsp1286I    BstXI    BstYI
     EaeI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    Hin4I
     MmeI     MscI     NdeI    PflMI    PpuMI     PsiI   PspOMI     SfcI
     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
    BciVI     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI
     BplI     BpmI    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PshAI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI