(Linear) (Six-base) MAPSORT of: KIEE0185.seq  Check: 4113  from: 1  to: 768



KIEE0185.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AflIII A'CryG_T

Cuts at:      0     505     507     768 
   Size:        505       2     261

  Fragments arranged by size: 

                 505     261       2
 
AleI CACnn'nnGTG

Cuts at:      0     401     768 
   Size:        401     367
 
ApoI r'AATT_y

Cuts at:      0     286     487     532     622     768 
   Size:        286     201      45      90     146

  Fragments arranged by size: 

                 286     201     146      90      45
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     727     760     768 
   Size:        727      33       8

  Fragments arranged by size: 

                 727      33       8
 
BclI T'GATC_A

Cuts at:      0      74     768 
   Size:         74     694
 
BlpI GC'TnA_GC

Cuts at:      0     681     768 
   Size:        681      87
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     292     293     768 
   Size:        292       1     475

  Fragments arranged by size: 

                 475     292       1
 
BsaAI yAC'GTr

Cuts at:      0     508     768 
   Size:        508     260
 
BsaBI GATnn'nnATC

Cuts at:      0      35     768 
   Size:         35     733
 
BsmI GAATG_Cn'

Cuts at:      0     103     768 
   Size:        103     665
 
BspHI T'CATG_A

Cuts at:      0     180     768 
   Size:        180     588
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      58     768 
   Size:         58     710
 
BsrDI GCAATG_nn'

Cuts at:      0     156     594     768 
   Size:        156     438     174

  Fragments arranged by size: 

                 438     174     156
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     155     768 
   Size:        155     613
 
DraI TTT'AAA

Cuts at:      0     545     768 
   Size:        545     223
 
DraIII CAC_nnn'GTG

Cuts at:      0      24     768 
   Size:         24     744
 
EcoRI G'AATT_C

Cuts at:      0     622     768 
   Size:        622     146
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     265     297     768 
   Size:        265      32     471

  Fragments arranged by size: 

                 471     265      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     191     554     768 
   Size:        191     363     214

  Fragments arranged by size: 

                 363     214     191
 
MslI CAynn'nnrTG

Cuts at:      0     401     631     768 
   Size:        401     230     137

  Fragments arranged by size: 

                 401     230     137
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     166     768 
   Size:        166     602
 
PmlI CAC'GTG

Cuts at:      0     508     768 
   Size:        508     260
 
PstI C_TGCA'G

Cuts at:      0     743     768 
   Size:        743      25
 
SfcI C'TryA_G

Cuts at:      0     739     768 
   Size:        739      29
 
SmlI C'TyrA_G

Cuts at:      0     271     308     768 
   Size:        271      37     460

  Fragments arranged by size: 

                 460     271      37
 
StyI C'CwwG_G

Cuts at:      0     160     768 
   Size:        160     608
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     568     600     768 
   Size:        568      32     168

  Fragments arranged by size: 

                 568     168      32
 
XbaI T'CTAG_A

Cuts at:      0     426     768 
   Size:        426     342
 
XmnI GAAnn'nnTTC

Cuts at:      0     487     662     768 
   Size:        487     175     106

  Fragments arranged by size: 

                 487     175     106
 

 Enzymes that do cut:

   AflIII     AleI     ApoI     BaeI     BclI     BlpI    BpuEI    BsaAI
    BsaBI     BsmI    BspHI    BspMI    BsrDI   BstAPI     DraI   DraIII
    EcoRI    Hin4I     MmeI     MslI    PflMI     PmlI     PstI     SfcI
     SmlI     StyI     TstI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XcmI     XhoI     XmaI     ZraI