Restriction map for
pF1KE0182
(Linear) (Six-base) MAPSORT of: KIEE0182.seq Check: 2014 from: 1 to: 678
KIEE0182.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 668 678
Size: 347 321 10
Fragments arranged by size:
347 321 10
AfeI AGC'GCT
Cuts at: 0 88 118 678
Size: 88 30 560
Fragments arranged by size:
560 88 30
AlwNI CAG_nnn'CTG
Cuts at: 0 567 646 678
Size: 567 79 32
Fragments arranged by size:
567 79 32
AvaI C'yCGr_G
Cuts at: 0 261 678
Size: 261 417
BanI G'GyrC_C
Cuts at: 0 151 678
Size: 151 527
BanII G_rGCy'C
Cuts at: 0 176 343 678
Size: 176 167 335
Fragments arranged by size:
335 176 167
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 364 678
Size: 248 116 314
Fragments arranged by size:
314 248 116
BbvCI CC'TCA_GC
Cuts at: 0 578 678
Size: 578 100
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 465 499 678
Size: 465 34 179
Fragments arranged by size:
465 179 34
BglI GCCn_nnn'nGGC
Cuts at: 0 641 650 678
Size: 641 9 28
Fragments arranged by size:
641 28 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 159 678
Size: 159 519
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 678
Size: 253 425
Bpu10I CC'TnA_GC
Cuts at: 0 578 678
Size: 578 100
BsaI GGTCTCn'nnnn_
Cuts at: 0 98 154 271 678
Size: 98 56 117 407
Fragments arranged by size:
407 117 98 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 151 181 678
Size: 151 30 497
Fragments arranged by size:
497 151 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 595 678
Size: 595 83
BsiEI CG_ry'CG
Cuts at: 0 100 678
Size: 100 578
BsmI GAATG_Cn'
Cuts at: 0 528 678
Size: 528 150
Bsp1286I G_dGCh'C
Cuts at: 0 176 343 678
Size: 176 167 335
Fragments arranged by size:
335 176 167
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 116 678
Size: 116 562
BsrDI GCAATG_nn'
Cuts at: 0 281 678
Size: 281 397
BsrFI r'CCGG_y
Cuts at: 0 95 678
Size: 95 583
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 336 462 678
Size: 48 288 126 216
Fragments arranged by size:
288 216 126 48
BtgI C'CryG_G
Cuts at: 0 155 678
Size: 155 523
BtsI GCAGTG_nn'
Cuts at: 0 276 678
Size: 276 402
DraIII CAC_nnn'GTG
Cuts at: 0 507 678
Size: 507 171
EaeI y'GGCC_r
Cuts at: 0 35 97 392 678
Size: 35 62 295 286
Fragments arranged by size:
295 286 62 35
EagI C'GGCC_G
Cuts at: 0 97 678
Size: 97 581
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 347 668 678
Size: 253 94 321 10
Fragments arranged by size:
321 253 94 10
EcoO109I rG'GnC_Cy
Cuts at: 0 643 678
Size: 643 35
HaeII r_GCGC'y
Cuts at: 0 90 120 678
Size: 90 30 558
Fragments arranged by size:
558 90 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 426 458 678
Size: 151 32 243 32 220
Fragments arranged by size:
243 220 151 32 32
HindIII A'AGCT_T
Cuts at: 0 319 678
Size: 319 359
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 428 678
Size: 334 94 250
Fragments arranged by size:
334 250 94
MscI TGG'CCA
Cuts at: 0 37 394 678
Size: 37 357 284
Fragments arranged by size:
357 284 37
MslI CAynn'nnrTG
Cuts at: 0 28 334 385 388 678
Size: 28 306 51 3 290
Fragments arranged by size:
306 290 51 28 3
NaeI GCC'GGC
Cuts at: 0 97 678
Size: 97 581
NcoI C'CATG_G
Cuts at: 0 155 678
Size: 155 523
NgoMIV G'CCGG_C
Cuts at: 0 95 678
Size: 95 583
PasI CC'CwG_GG
Cuts at: 0 410 678
Size: 410 268
PfoI T'CCnGG_A
Cuts at: 0 461 678
Size: 461 217
PstI C_TGCA'G
Cuts at: 0 116 662 678
Size: 116 546 16
Fragments arranged by size:
546 116 16
SbfI CC_TGCA'GG
Cuts at: 0 662 678
Size: 662 16
SexAI A'CCwGG_T
Cuts at: 0 503 678
Size: 503 175
SfcI C'TryA_G
Cuts at: 0 112 658 678
Size: 112 546 20
Fragments arranged by size:
546 112 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 641 678
Size: 641 37
StuI AGG'CCT
Cuts at: 0 545 635 678
Size: 545 90 43
Fragments arranged by size:
545 90 43
StyI C'CwwG_G
Cuts at: 0 65 155 678
Size: 65 90 523
Fragments arranged by size:
523 90 65
Tth111I GACn'n_nGTC
Cuts at: 0 165 196 678
Size: 165 31 482
Fragments arranged by size:
482 165 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 30 45 678
Size: 30 15 633
Fragments arranged by size:
633 30 15
XmnI GAAnn'nnTTC
Cuts at: 0 596 678
Size: 596 82
Enzymes that do cut:
AcuI AfeI AlwNI AvaI BanI BanII BbsI BbvCI
BcgI BglI BmrI BpmI Bpu10I BsaI BsaXI BseRI
BsiEI BsmI Bsp1286I BspMI BsrDI BsrFI BstXI BtgI
BtsI DraIII EaeI EagI Eco57MI EcoO109I HaeII Hin4I
HindIII MmeI MscI MslI NaeI NcoI NgoMIV PasI
PfoI PstI SbfI SexAI SfcI SfiI StuI StyI
Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI BpuEI
BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiHKAI BsiWI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI HincII HpaI KasI KpnI
MfeI MluI MspA1I NarI NdeI NheI NotI NruI
NsiI NspI PacI PciI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI ScaI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TatI TstI XbaI XhoI XmaI
ZraI