(Linear) (Six-base) MAPSORT of: KIEE0169.seq  Check: 9038  from: 1  to: 579



KIEE0169.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     437     579 
   Size:        297     140     142

  Fragments arranged by size: 

                 297     142     140
 
AlwNI CAG_nnn'CTG

Cuts at:      0     300     579 
   Size:        300     279
 
ApoI r'AATT_y

Cuts at:      0     187     250     579 
   Size:        187      63     329

  Fragments arranged by size: 

                 329     187      63
 
AvaI C'yCGr_G

Cuts at:      0     558     579 
   Size:        558      21
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     195     579 
   Size:        195     384
 
BglII A'GATC_T

Cuts at:      0     452     579 
   Size:        452     127
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     110     579 
   Size:        110     469
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     310     579 
   Size:        310     269
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     285     579 
   Size:        123     162     294

  Fragments arranged by size: 

                 294     162     123
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     527     579 
   Size:        527      52
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     579 
   Size:        394     185
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     473     579 
   Size:        473     106
 
BsrFI r'CCGG_y

Cuts at:      0     568     579 
   Size:        568      11
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      45     579 
   Size:         45     534
 
BstYI r'GATC_y

Cuts at:      0      77     452     579 
   Size:         77     375     127

  Fragments arranged by size: 

                 375     127      77
 
BtgI C'CryG_G

Cuts at:      0     494     579 
   Size:        494      85
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     243     579 
   Size:        243     336
 
BtsI GCAGTG_nn'

Cuts at:      0     556     570     579 
   Size:        556      14       9

  Fragments arranged by size: 

                 556      14       9
 
EaeI y'GGCC_r

Cuts at:      0     570     579 
   Size:        570       9
 
EarI CTCTTCn'nnn_

Cuts at:      0     131     494     579 
   Size:        131     363      85

  Fragments arranged by size: 

                 363     131      85
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     448     579 
   Size:        448     131
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     297     310     437     579 
   Size:        297      13     127     142

  Fragments arranged by size: 

                 297     142     127      13
 
EcoRI G'AATT_C

Cuts at:      0     250     579 
   Size:        250     329
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     239     271     579 
   Size:        239      32     308

  Fragments arranged by size: 

                 308     239      32
 
HincII GTy'rAC

Cuts at:      0     428     579 
   Size:        428     151
 
NaeI GCC'GGC

Cuts at:      0     570     579 
   Size:        570       9
 
NcoI C'CATG_G

Cuts at:      0     494     579 
   Size:        494      85
 
NgoMIV G'CCGG_C

Cuts at:      0     568     579 
   Size:        568      11
 
NspI r_CATG'y

Cuts at:      0     202     321     579 
   Size:        202     119     258

  Fragments arranged by size: 

                 258     202     119
 
PfoI T'CCnGG_A

Cuts at:      0      80     579 
   Size:         80     499
 
PshAI GACnn'nnGTC

Cuts at:      0     126     579 
   Size:        126     453
 
PstI C_TGCA'G

Cuts at:      0     551     579 
   Size:        551      28
 
SfcI C'TryA_G

Cuts at:      0     547     579 
   Size:        547      32
 
SmlI C'TyrA_G

Cuts at:      0     102     264     579 
   Size:        102     162     315

  Fragments arranged by size: 

                 315     162     102
 
StyI C'CwwG_G

Cuts at:      0     494     579 
   Size:        494      85
 
XmnI GAAnn'nnTTC

Cuts at:      0     163     579 
   Size:        163     416
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     AvaI     BbsI    BglII     BmrI     BpmI
    BpuEI    BseRI     BsgI    BsmBI    BsrFI   BstAPI    BstYI     BtgI
    BtgZI     BtsI     EaeI     EarI     EciI  Eco57MI    EcoRI    Hin4I
   HincII     NaeI     NcoI   NgoMIV     NspI     PfoI    PshAI     PstI
     SfcI     SmlI     StyI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BssSI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI     DraI
   DraIII     DrdI     EagI  EcoICRI    EcoNI EcoO109I    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NarI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI