Restriction map for
pF1KE0167
(Linear) (Six-base) MAPSORT of: KIEE0167.seq Check: 198 from: 1 to: 492
KIEE0167.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
Acc65I G'GTAC_C
Cuts at: 0 119 492
Size: 119 373
AflIII A'CryG_T
Cuts at: 0 281 492
Size: 281 211
ApaLI G'TGCA_C
Cuts at: 0 403 492
Size: 403 89
AvaI C'yCGr_G
Cuts at: 0 37 160 492
Size: 37 123 332
Fragments arranged by size:
332 123 37
BanI G'GyrC_C
Cuts at: 0 119 492
Size: 119 373
BanII G_rGCy'C
Cuts at: 0 161 492
Size: 161 331
BbsI GAAGACnn'nnnn_
Cuts at: 0 237 492
Size: 237 255
Bme1580I G_kGCm'C
Cuts at: 0 407 492
Size: 407 85
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 370 439 492
Size: 331 39 69 53
Fragments arranged by size:
331 69 53 39
BsaAI yAC'GTr
Cuts at: 0 282 492
Size: 282 210
BsaBI GATnn'nnATC
Cuts at: 0 485 492
Size: 485 7
BsaHI Gr'CG_yC
Cuts at: 0 242 492
Size: 242 250
BsaWI w'CCGG_w
Cuts at: 0 74 492
Size: 74 418
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 181 492
Size: 178 3 311
Fragments arranged by size:
311 178 3
BseYI C'CCAG_C
Cuts at: 0 432 492
Size: 432 60
BsiHKAI G_wGCw'C
Cuts at: 0 407 492
Size: 407 85
BsiWI C'GTAC_G
Cuts at: 0 278 492
Size: 278 214
Bsp1286I G_dGCh'C
Cuts at: 0 161 407 492
Size: 161 246 85
Fragments arranged by size:
246 161 85
BspEI T'CCGG_A
Cuts at: 0 74 492
Size: 74 418
BsrFI r'CCGG_y
Cuts at: 0 116 230 492
Size: 116 114 262
Fragments arranged by size:
262 116 114
BstBI TT'CG_AA
Cuts at: 0 248 492
Size: 248 244
BtgI C'CryG_G
Cuts at: 0 461 492
Size: 461 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 370 439 492
Size: 331 39 69 53
Fragments arranged by size:
331 69 53 39
EcoO109I rG'GnC_Cy
Cuts at: 0 96 492
Size: 96 396
KpnI G_GTAC'C
Cuts at: 0 123 492
Size: 123 369
MspA1I CmG'CkG
Cuts at: 0 131 492
Size: 131 361
NaeI GCC'GGC
Cuts at: 0 232 492
Size: 232 260
NcoI C'CATG_G
Cuts at: 0 461 492
Size: 461 31
NgoMIV G'CCGG_C
Cuts at: 0 230 492
Size: 230 262
PflMI CCAn_nnn'nTGG
Cuts at: 0 451 492
Size: 451 41
PpuMI rG'GwC_Cy
Cuts at: 0 96 492
Size: 96 396
RsrII CG'GwC_CG
Cuts at: 0 126 186 492
Size: 126 60 306
Fragments arranged by size:
306 126 60
StyI C'CwwG_G
Cuts at: 0 461 492
Size: 461 31
TatI w'GTAC_w
Cuts at: 0 288 492
Size: 288 204
Tth111I GACn'n_nGTC
Cuts at: 0 94 492
Size: 94 398
Enzymes that do cut:
Acc65I AflIII ApaLI AvaI BanI BanII BbsI Bme1580I
BpmI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiHKAI
BsiWI Bsp1286I BspEI BsrFI BstBI BtgI Eco57MI EcoO109I
KpnI MspA1I NaeI NcoI NgoMIV PflMI PpuMI RsrII
StyI TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaXI BsgI BsiEI
BsmI BsmBI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
MfeI MluI MmeI MscI MslI NarI NdeI NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI XbaI XcmI XhoI XmaI XmnI
ZraI