Restriction map for
pF1KE0161
(Linear) (Six-base) MAPSORT of: KIEE0161.seq Check: 6517 from: 1 to: 795
KIEE0161.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 187 795
Size: 187 608
AatII G_ACGT'C
Cuts at: 0 425 795
Size: 425 370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 795
Size: 215 580
AhdI GACnn_n'nnGTC
Cuts at: 0 225 795
Size: 225 570
AleI CACnn'nnGTG
Cuts at: 0 535 795
Size: 535 260
AlwNI CAG_nnn'CTG
Cuts at: 0 204 795
Size: 204 591
ApaI G_GGCC'C
Cuts at: 0 340 795
Size: 340 455
BamHI G'GATC_C
Cuts at: 0 438 795
Size: 438 357
BanI G'GyrC_C
Cuts at: 0 171 795
Size: 171 624
BanII G_rGCy'C
Cuts at: 0 340 795
Size: 340 455
BbsI GAAGACnn'nnnn_
Cuts at: 0 221 252 795
Size: 221 31 543
Fragments arranged by size:
543 221 31
BbvCI CC'TCA_GC
Cuts at: 0 115 795
Size: 115 680
BfrBI ATG'CAT
Cuts at: 0 494 795
Size: 494 301
Bme1580I G_kGCm'C
Cuts at: 0 176 340 795
Size: 176 164 455
Fragments arranged by size:
455 176 164
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 669 795
Size: 669 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 639 795
Size: 86 553 156
Fragments arranged by size:
553 156 86
Bpu10I CC'TnA_GC
Cuts at: 0 115 795
Size: 115 680
BsaI GGTCTCn'nnnn_
Cuts at: 0 592 795
Size: 592 203
BsaHI Gr'CG_yC
Cuts at: 0 422 795
Size: 422 373
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 213 795
Size: 213 582
BsmI GAATG_Cn'
Cuts at: 0 609 795
Size: 609 186
Bsp1286I G_dGCh'C
Cuts at: 0 176 340 795
Size: 176 164 455
Fragments arranged by size:
455 176 164
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 187 795
Size: 187 608
BsrFI r'CCGG_y
Cuts at: 0 601 795
Size: 601 194
BstAPI GCAn_nnn'nTGC
Cuts at: 0 37 795
Size: 37 758
BstYI r'GATC_y
Cuts at: 0 438 636 795
Size: 438 198 159
Fragments arranged by size:
438 198 159
BtsI GCAGTG_nn'
Cuts at: 0 711 795
Size: 711 84
DraIII CAC_nnn'GTG
Cuts at: 0 159 795
Size: 159 636
DrdI GACnn_nn'nnGTC
Cuts at: 0 355 795
Size: 355 440
EaeI y'GGCC_r
Cuts at: 0 281 753 795
Size: 281 472 42
Fragments arranged by size:
472 281 42
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 86 215 639 795
Size: 86 129 424 156
Fragments arranged by size:
424 156 129 86
EcoO109I rG'GnC_Cy
Cuts at: 0 336 795
Size: 336 459
EcoRV GAT'ATC
Cuts at: 0 628 795
Size: 628 167
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 211 243 795
Size: 3 32 176 32 552
Fragments arranged by size:
552 176 32 32 3
HincII GTy'rAC
Cuts at: 0 96 795
Size: 96 699
HindIII A'AGCT_T
Cuts at: 0 522 795
Size: 522 273
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 795
Size: 509 286
MscI TGG'CCA
Cuts at: 0 755 795
Size: 755 40
MslI CAynn'nnrTG
Cuts at: 0 535 795
Size: 535 260
NdeI CA'TA_TG
Cuts at: 0 580 795
Size: 580 215
NsiI A_TGCA'T
Cuts at: 0 496 795
Size: 496 299
NspI r_CATG'y
Cuts at: 0 53 795
Size: 53 742
PspOMI G'GGCC_C
Cuts at: 0 336 795
Size: 336 459
SexAI A'CCwGG_T
Cuts at: 0 155 795
Size: 155 640
SfcI C'TryA_G
Cuts at: 0 362 795
Size: 362 433
StuI AGG'CCT
Cuts at: 0 71 795
Size: 71 724
StyI C'CwwG_G
Cuts at: 0 443 795
Size: 443 352
Tth111I GACn'n_nGTC
Cuts at: 0 91 795
Size: 91 704
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 691 763 795
Size: 492 199 72 32
Fragments arranged by size:
492 199 72 32
ZraI GAC'GTC
Cuts at: 0 423 795
Size: 423 372
Enzymes that do cut:
AarI AatII AcuI AhdI AleI AlwNI ApaI BamHI
BanI BanII BbsI BbvCI BfrBI Bme1580I BplI BpmI
Bpu10I BsaI BsaHI BseRI BsmI Bsp1286I BspMI BsrFI
BstAPI BstYI BtsI DraIII DrdI EaeI Eco57MI EcoO109I
EcoRV Hin4I HincII HindIII MmeI MscI MslI NdeI
NsiI NspI PspOMI SexAI SfcI StuI StyI Tth111I
XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BclI BglI BglII BlpI
BmgBI BmrI BmtI BpuEI BsaAI BsaBI BsaWI BsaXI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI EagI
EarI EciI EcoICRI EcoNI EcoRI FalI FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MspA1I
NaeI NarI NcoI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TaqII TatI TstI XbaI XhoI
XmaI XmnI