Restriction map for
pF1KE0156
(Linear) (Six-base) MAPSORT of: KIEE0156.seq Check: 6222 from: 1 to: 783
KIEE0156.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 783
Size: 746 37
AflIII A'CryG_T
Cuts at: 0 224 341 783
Size: 224 117 442
Fragments arranged by size:
442 224 117
AvaI C'yCGr_G
Cuts at: 0 194 551 783
Size: 194 357 232
Fragments arranged by size:
357 232 194
BamHI G'GATC_C
Cuts at: 0 572 783
Size: 572 211
BanI G'GyrC_C
Cuts at: 0 248 388 783
Size: 248 140 395
Fragments arranged by size:
395 248 140
BciVI GTATCCnnnnn_n'
Cuts at: 0 730 783
Size: 730 53
BglI GCCn_nnn'nGGC
Cuts at: 0 217 783
Size: 217 566
Bme1580I G_kGCm'C
Cuts at: 0 391 783
Size: 391 392
BmgBI CAC'GTC
Cuts at: 0 225 783
Size: 225 558
BmrI ACTGGGnnnn_n'
Cuts at: 0 366 783
Size: 366 417
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 783
Size: 542 241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 783
Size: 367 416
BsaHI Gr'CG_yC
Cuts at: 0 4 783
Size: 4 779
BseYI C'CCAG_C
Cuts at: 0 162 783
Size: 162 621
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 322 783
Size: 286 36 461
Fragments arranged by size:
461 286 36
BsiEI CG_ry'CG
Cuts at: 0 72 783
Size: 72 711
BsmI GAATG_Cn'
Cuts at: 0 712 783
Size: 712 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 11 142 783
Size: 11 131 641
Fragments arranged by size:
641 131 11
Bsp1286I G_dGCh'C
Cuts at: 0 391 783
Size: 391 392
BsrBI CCG'CTC
Cuts at: 0 29 62 76 783
Size: 29 33 14 707
Fragments arranged by size:
707 33 29 14
BsrDI GCAATG_nn'
Cuts at: 0 602 783
Size: 602 181
BstYI r'GATC_y
Cuts at: 0 572 783
Size: 572 211
Bsu36I CC'TnA_GG
Cuts at: 0 689 783
Size: 689 94
EaeI y'GGCC_r
Cuts at: 0 69 92 783
Size: 69 23 691
Fragments arranged by size:
691 69 23
EagI C'GGCC_G
Cuts at: 0 69 783
Size: 69 714
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 746 783
Size: 542 204 37
Fragments arranged by size:
542 204 37
EcoO109I rG'GnC_Cy
Cuts at: 0 684 749 783
Size: 684 65 34
Fragments arranged by size:
684 65 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 408 783
Size: 408 375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 557 589 783
Size: 557 32 194
Fragments arranged by size:
557 194 32
HincII GTy'rAC
Cuts at: 0 378 783
Size: 378 405
MspA1I CmG'CkG
Cuts at: 0 139 578 783
Size: 139 439 205
Fragments arranged by size:
439 205 139
NspI r_CATG'y
Cuts at: 0 345 783
Size: 345 438
PasI CC'CwG_GG
Cuts at: 0 595 783
Size: 595 188
PciI A'CATG_T
Cuts at: 0 341 783
Size: 341 442
PpuMI rG'GwC_Cy
Cuts at: 0 684 749 783
Size: 684 65 34
Fragments arranged by size:
684 65 34
PstI C_TGCA'G
Cuts at: 0 414 783
Size: 414 369
SanDI GG'GwC_CC
Cuts at: 0 684 783
Size: 684 99
SfcI C'TryA_G
Cuts at: 0 410 783
Size: 410 373
SmaI CCC'GGG
Cuts at: 0 553 783
Size: 553 230
SmlI C'TyrA_G
Cuts at: 0 382 783
Size: 382 401
StuI AGG'CCT
Cuts at: 0 271 783
Size: 271 512
StyI C'CwwG_G
Cuts at: 0 41 515 783
Size: 41 474 268
Fragments arranged by size:
474 268 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 334 627 783
Size: 334 293 156
Fragments arranged by size:
334 293 156
Tth111I GACn'n_nGTC
Cuts at: 0 364 783
Size: 364 419
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 783
Size: 252 531
XmaI C'CCGG_G
Cuts at: 0 551 783
Size: 551 232
Enzymes that do cut:
AcuI AflIII AvaI BamHI BanI BciVI BglI Bme1580I
BmgBI BmrI BpmI BpuEI BsaHI BseYI BsgI BsiEI
BsmI BsmBI Bsp1286I BsrBI BsrDI BstYI Bsu36I EaeI
EagI Eco57MI EcoO109I FalI Hin4I HincII MspA1I NspI
PasI PciI PpuMI PstI SanDI SfcI SmaI SmlI
StuI StyI TaqII Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BanII BbeI BbsI
BbvCI BcgI BclI BfrBI BglII BlpI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BseRI BsiHKAI
BsiWI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EarI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TatI TstI XbaI XhoI XmnI ZraI