(Linear) (Six-base) MAPSORT of: KIEE0152.seq  Check: 7885  from: 1  to: 393



KIEE0152.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     335     393 
   Size:        335      58
 
AlwNI CAG_nnn'CTG

Cuts at:      0      57     393 
   Size:         57     336
 
AvaI C'yCGr_G

Cuts at:      0     325     393 
   Size:        325      68
 
BclI T'GATC_A

Cuts at:      0      59     393 
   Size:         59     334
 
BglII A'GATC_T

Cuts at:      0      93     393 
   Size:         93     300
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     393 
   Size:        307      86
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     179     393 
   Size:        179     214
 
BsaBI GATnn'nnATC

Cuts at:      0      29     393 
   Size:         29     364
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     128     393 
   Size:        128     265
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     300     393 
   Size:        300      93
 
BstYI r'GATC_y

Cuts at:      0      93     393 
   Size:         93     300
 
EaeI y'GGCC_r

Cuts at:      0       2     393 
   Size:          2     391
 
EarI CTCTTCn'nnn_

Cuts at:      0     302     393 
   Size:        302      91
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     393 
   Size:        251     142
 
PfoI T'CCnGG_A

Cuts at:      0      79     393 
   Size:         79     314
 
SapI GCTCTTCn'nnn_

Cuts at:      0     302     393 
   Size:        302      91
 
SmlI C'TyrA_G

Cuts at:      0     286     393 
   Size:        286     107
 
StuI AGG'CCT

Cuts at:      0      48     393 
   Size:         48     345
 

 Enzymes that do cut:

     AlfI    AlwNI     AvaI     BclI    BglII    BpuEI     BsaI    BsaBI
    BseRI   BstAPI    BstYI     EaeI     EarI     MmeI     PfoI     SapI
     SmlI     StuI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaAI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI  BstZ17I
   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI