Restriction map for
pF1KE0146
(Linear) (Six-base) MAPSORT of: KIEE0146.seq Check: 7894 from: 1 to: 888
KIEE0146.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
Acc65I G'GTAC_C
Cuts at: 0 630 662 888
Size: 630 32 226
Fragments arranged by size:
630 226 32
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 710 888
Size: 461 249 178
Fragments arranged by size:
461 249 178
AflII C'TTAA_G
Cuts at: 0 871 888
Size: 871 17
ApaI G_GGCC'C
Cuts at: 0 30 36 888
Size: 30 6 852
Fragments arranged by size:
852 30 6
ApoI r'AATT_y
Cuts at: 0 682 888
Size: 682 206
AvaI C'yCGr_G
Cuts at: 0 29 888
Size: 29 859
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 289 322 888
Size: 289 33 566
Fragments arranged by size:
566 289 33
BamHI G'GATC_C
Cuts at: 0 672 780 888
Size: 672 108 108
Fragments arranged by size:
672 108 108
BanI G'GyrC_C
Cuts at: 0 630 662 888
Size: 630 32 226
Fragments arranged by size:
630 226 32
BanII G_rGCy'C
Cuts at: 0 30 36 888
Size: 30 6 852
Fragments arranged by size:
852 30 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 120 888
Size: 120 768
Bme1580I G_kGCm'C
Cuts at: 0 30 36 888
Size: 30 6 852
Fragments arranged by size:
852 30 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 794 888
Size: 794 94
BsaBI GATnn'nnATC
Cuts at: 0 863 888
Size: 863 25
BsaHI Gr'CG_yC
Cuts at: 0 270 888
Size: 270 618
BsaWI w'CCGG_w
Cuts at: 0 105 888
Size: 105 783
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 120 150 888
Size: 120 30 738
Fragments arranged by size:
738 120 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 888
Size: 439 449
BseYI C'CCAG_C
Cuts at: 0 356 888
Size: 356 532
BsmI GAATG_Cn'
Cuts at: 0 614 888
Size: 614 274
Bsp1286I G_dGCh'C
Cuts at: 0 30 36 888
Size: 30 6 852
Fragments arranged by size:
852 30 6
BsrFI r'CCGG_y
Cuts at: 0 4 888
Size: 4 884
BstYI r'GATC_y
Cuts at: 0 672 780 888
Size: 672 108 108
Fragments arranged by size:
672 108 108
BtgI C'CryG_G
Cuts at: 0 657 888
Size: 657 231
DrdI GACnn_nn'nnGTC
Cuts at: 0 122 888
Size: 122 766
EaeI y'GGCC_r
Cuts at: 0 2 71 830 888
Size: 2 69 759 58
Fragments arranged by size:
759 69 58 2
EarI CTCTTCn'nnn_
Cuts at: 0 126 760 888
Size: 126 634 128
Fragments arranged by size:
634 128 126
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 685 888
Size: 685 203
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 461 710 794 888
Size: 461 249 84 94
Fragments arranged by size:
461 249 94 84
EcoO109I rG'GnC_Cy
Cuts at: 0 26 33 51 888
Size: 26 7 18 837
Fragments arranged by size:
837 26 18 7
EcoRV GAT'ATC
Cuts at: 0 738 888
Size: 738 150
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 715 888
Size: 715 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 689 721 888
Size: 689 32 167
Fragments arranged by size:
689 167 32
HincII GTy'rAC
Cuts at: 0 340 888
Size: 340 548
KpnI G_GTAC'C
Cuts at: 0 634 666 888
Size: 634 32 222
Fragments arranged by size:
634 222 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 888
Size: 106 782
MscI TGG'CCA
Cuts at: 0 73 888
Size: 73 815
MspA1I CmG'CkG
Cuts at: 0 417 888
Size: 417 471
PfoI T'CCnGG_A
Cuts at: 0 810 888
Size: 810 78
PspOMI G'GGCC_C
Cuts at: 0 26 32 888
Size: 26 6 856
Fragments arranged by size:
856 26 6
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 646 678 888
Size: 646 32 210
Fragments arranged by size:
646 210 32
PstI C_TGCA'G
Cuts at: 0 305 888
Size: 305 583
PvuII CAG'CTG
Cuts at: 0 417 888
Size: 417 471
SfcI C'TryA_G
Cuts at: 0 301 524 854 888
Size: 301 223 330 34
Fragments arranged by size:
330 301 223 34
SmaI CCC'GGG
Cuts at: 0 31 888
Size: 31 857
SmlI C'TyrA_G
Cuts at: 0 871 888
Size: 871 17
SrfI GCCC'GGGC
Cuts at: 0 31 888
Size: 31 857
SspI AAT'ATT
Cuts at: 0 459 888
Size: 459 429
StyI C'CwwG_G
Cuts at: 0 784 888
Size: 784 104
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 807 888
Size: 807 81
TatI w'GTAC_w
Cuts at: 0 465 769 888
Size: 465 304 119
Fragments arranged by size:
465 304 119
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 806 838 888
Size: 806 32 50
Fragments arranged by size:
806 50 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 364 888
Size: 364 524
XmaI C'CCGG_G
Cuts at: 0 29 888
Size: 29 859
Enzymes that do cut:
Acc65I AcuI AflII ApaI ApoI AvaI BaeI BamHI
BanI BanII BbsI Bme1580I BpmI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsmI Bsp1286I BsrFI BstYI BtgI
DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRV FalI
Hin4I HincII KpnI MmeI MscI MspA1I PfoI PspOMI
PsrI PstI PvuII SfcI SmaI SmlI SrfI SspI
StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaLI AscI AseI AsiSI
AvrII BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaI BsaAI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI StuI
SwaI Tth111I XbaI XhoI XmnI ZraI