Restriction map for
pF1KE0142
(Linear) (Six-base) MAPSORT of: KIEE0142.seq Check: 9864 from: 1 to: 537
KIEE0142.orf
With 182 enzymes: SgfI *
April 24, 2009 13:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 405 537
Size: 405 132
AfeI AGC'GCT
Cuts at: 0 404 537
Size: 404 133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 365 481 537
Size: 365 116 56
Fragments arranged by size:
365 116 56
AlwNI CAG_nnn'CTG
Cuts at: 0 523 537
Size: 523 14
BanI G'GyrC_C
Cuts at: 0 305 426 439 537
Size: 305 121 13 98
Fragments arranged by size:
305 121 98 13
BbeI G_GCGC'C
Cuts at: 0 309 430 443 537
Size: 309 121 13 94
Fragments arranged by size:
309 121 94 13
Bme1580I G_kGCm'C
Cuts at: 0 196 537
Size: 196 341
BmrI ACTGGGnnnn_n'
Cuts at: 0 159 537
Size: 159 378
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 466 537
Size: 316 150 71
Fragments arranged by size:
316 150 71
BsaBI GATnn'nnATC
Cuts at: 0 175 537
Size: 175 362
BsaHI Gr'CG_yC
Cuts at: 0 306 427 440 537
Size: 306 121 13 97
Fragments arranged by size:
306 121 97 13
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 349 352 537
Size: 232 117 3 185
Fragments arranged by size:
232 185 117 3
BseYI C'CCAG_C
Cuts at: 0 234 249 482 537
Size: 234 15 233 55
Fragments arranged by size:
234 233 55 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 537
Size: 439 98
BsiEI CG_ry'CG
Cuts at: 0 501 537
Size: 501 36
Bsp1286I G_dGCh'C
Cuts at: 0 196 537
Size: 196 341
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 42 405 537
Size: 42 363 132
Fragments arranged by size:
363 132 42
BsrFI r'CCGG_y
Cuts at: 0 132 500 537
Size: 132 368 37
Fragments arranged by size:
368 132 37
BssHII G'CGCG_C
Cuts at: 0 477 537
Size: 477 60
BstAPI GCAn_nnn'nTGC
Cuts at: 0 474 523 537
Size: 474 49 14
Fragments arranged by size:
474 49 14
BtsI GCAGTG_nn'
Cuts at: 0 524 537
Size: 524 13
DraIII CAC_nnn'GTG
Cuts at: 0 153 519 537
Size: 153 366 18
Fragments arranged by size:
366 153 18
EaeI y'GGCC_r
Cuts at: 0 498 537
Size: 498 39
EagI C'GGCC_G
Cuts at: 0 498 537
Size: 498 39
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 466 537
Size: 316 150 71
Fragments arranged by size:
316 150 71
FspI TGC'GCA
Cuts at: 0 175 457 537
Size: 175 282 80
Fragments arranged by size:
282 175 80
FspAI rTGC'GCAy
Cuts at: 0 175 537
Size: 175 362
HaeII r_GCGC'y
Cuts at: 0 309 327 391 397 406 430 443 537
Size: 309 18 64 6 9 24 13 94
Fragments arranged by size:
309 94 64 24 18 13 9 6
KasI G'GCGC_C
Cuts at: 0 305 426 439 537
Size: 305 121 13 98
Fragments arranged by size:
305 121 98 13
MspA1I CmG'CkG
Cuts at: 0 210 537
Size: 210 327
NaeI GCC'GGC
Cuts at: 0 134 537
Size: 134 403
NarI GG'CG_CC
Cuts at: 0 306 427 440 537
Size: 306 121 13 97
Fragments arranged by size:
306 121 97 13
NgoMIV G'CCGG_C
Cuts at: 0 132 537
Size: 132 405
PstI C_TGCA'G
Cuts at: 0 410 537
Size: 410 127
RsrII CG'GwC_CG
Cuts at: 0 503 537
Size: 503 34
ScaI AGT'ACT
Cuts at: 0 301 537
Size: 301 236
SfcI C'TryA_G
Cuts at: 0 406 537
Size: 406 131
SfoI GGC'GCC
Cuts at: 0 307 428 441 537
Size: 307 121 13 96
Fragments arranged by size:
307 121 96 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 141 537
Size: 141 396
TatI w'GTAC_w
Cuts at: 0 299 537
Size: 299 238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 270 537
Size: 270 267
Enzymes that do cut:
AarI AfeI AlfI AlwNI BanI BbeI Bme1580I BmrI
BpmI BsaBI BsaHI BseRI BseYI BsgI BsiEI Bsp1286I
BspMI BsrFI BssHII BstAPI BtsI DraIII EaeI EagI
Eco57MI FspI FspAI HaeII KasI MspA1I NaeI NarI
NgoMIV PstI RsrII ScaI SfcI SfoI TaqII TatI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DrdI EarI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI