(Linear) (Six-base) MAPSORT of: KIEE0139.seq  Check: 8581  from: 1  to: 540



KIEE0139.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:11  ..

AccI GT'mk_AC

Cuts at:      0     434     540 
   Size:        434     106
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     540 
   Size:        122     418
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     420     540 
   Size:        420     120
 
ApoI r'AATT_y

Cuts at:      0     139     540 
   Size:        139     401
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     477     540 
   Size:        477      63
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     187     540 
   Size:        187     353
 
BsaWI w'CCGG_w

Cuts at:      0     149     540 
   Size:        149     391
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     160     190     540 
   Size:        160      30     350

  Fragments arranged by size: 

                 350     160      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      75     540 
   Size:         75     465
 
BsiEI CG_ry'CG

Cuts at:      0      13     540 
   Size:         13     527
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      47     540 
   Size:         47     493
 
BsrBI CCG'CTC

Cuts at:      0     195     540 
   Size:        195     345
 
BsrFI r'CCGG_y

Cuts at:      0      87     540 
   Size:         87     453
 
BssSI C'ACGA_G

Cuts at:      0     320     540 
   Size:        320     220
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     240     540 
   Size:        240     300
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     363     449     540 
   Size:        363      86      91

  Fragments arranged by size: 

                 363      91      86
 
BtgI C'CryG_G

Cuts at:      0     448     540 
   Size:        448      92
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     219     540 
   Size:        219     321
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     122     187     540 
   Size:        122      65     353

  Fragments arranged by size: 

                 353     122      65
 
EcoRI G'AATT_C

Cuts at:      0     139     540 
   Size:        139     401
 
HaeII r_GCGC'y

Cuts at:      0       7     540 
   Size:          7     533
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     160     192     246     278     540 
   Size:        160      32      54      32     262

  Fragments arranged by size: 

                 262     160      54      32      32
 
HincII GTy'rAC

Cuts at:      0      74     540 
   Size:         74     466
 
MslI CAynn'nnrTG

Cuts at:      0     290     402     540 
   Size:        290     112     138

  Fragments arranged by size: 

                 290     138     112
 
NaeI GCC'GGC

Cuts at:      0      89     540 
   Size:         89     451
 
NgoMIV G'CCGG_C

Cuts at:      0      87     540 
   Size:         87     453
 
PasI CC'CwG_GG

Cuts at:      0      49     540 
   Size:         49     491
 
PsiI TTA'TAA

Cuts at:      0     250     540 
   Size:        250     290
 
SfcI C'TryA_G

Cuts at:      0     179     540 
   Size:        179     361
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      41     540 
   Size:         41     499
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     363     540 
   Size:        363     177
 

 Enzymes that do cut:

     AccI     AcuI     AlfI     ApoI     BplI     BpmI    BsaWI    BsaXI
     BsgI    BsiEI    BspMI    BsrBI    BsrFI    BssSI   BstAPI    BstXI
     BtgI     EciI  Eco57MI    EcoRI    HaeII    Hin4I   HincII     MslI
     NaeI   NgoMIV     PasI     PsiI     SfcI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BseRI    BseYI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrDI    BsrGI   BssHII    BstBI
   BstEII    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI    EcoNI
 EcoO109I    EcoRV     FalI     FseI     FspI    FspAI  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NarI
     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI