Restriction map for
pF1KE0136
(Linear) (Six-base) MAPSORT of: KIEE0136.seq Check: 1665 from: 1 to: 906
KIEE0136.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 102 906
Size: 102 804
AatII G_ACGT'C
Cuts at: 0 365 906
Size: 365 541
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 906
Size: 835 71
AfeI AGC'GCT
Cuts at: 0 703 906
Size: 703 203
AflIII A'CryG_T
Cuts at: 0 341 906
Size: 341 565
AleI CACnn'nnGTG
Cuts at: 0 649 906
Size: 649 257
AlwNI CAG_nnn'CTG
Cuts at: 0 84 330 906
Size: 84 246 576
Fragments arranged by size:
576 246 84
ApaI G_GGCC'C
Cuts at: 0 48 242 906
Size: 48 194 664
Fragments arranged by size:
664 194 48
AvaI C'yCGr_G
Cuts at: 0 371 906
Size: 371 535
BanII G_rGCy'C
Cuts at: 0 48 242 906
Size: 48 194 664
Fragments arranged by size:
664 194 48
BbvCI CC'TCA_GC
Cuts at: 0 887 906
Size: 887 19
BciVI GTATCCnnnnn_n'
Cuts at: 0 128 906
Size: 128 778
BglI GCCn_nnn'nGGC
Cuts at: 0 43 100 906
Size: 43 57 806
Fragments arranged by size:
806 57 43
BlpI GC'TnA_GC
Cuts at: 0 820 906
Size: 820 86
Bme1580I G_kGCm'C
Cuts at: 0 48 242 898 906
Size: 48 194 656 8
Fragments arranged by size:
656 194 48 8
BmgBI CAC'GTC
Cuts at: 0 528 906
Size: 528 378
BmrI ACTGGGnnnn_n'
Cuts at: 0 384 630 906
Size: 384 246 276
Fragments arranged by size:
384 276 246
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 613 906
Size: 517 96 293
Fragments arranged by size:
517 293 96
Bpu10I CC'TnA_GC
Cuts at: 0 253 887 906
Size: 253 634 19
Fragments arranged by size:
634 253 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 507 906
Size: 507 399
BsaAI yAC'GTr
Cuts at: 0 342 906
Size: 342 564
BsaHI Gr'CG_yC
Cuts at: 0 362 906
Size: 362 544
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 781 784 906
Size: 10 771 3 122
Fragments arranged by size:
771 122 10 3
BseYI C'CCAG_C
Cuts at: 0 77 263 267 403 418 506 906
Size: 77 186 4 136 15 88 400
Fragments arranged by size:
400 186 136 88 77 15 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 654 676 703 906
Size: 424 230 22 27 203
Fragments arranged by size:
424 230 203 27 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 283 906
Size: 283 623
Bsp1286I G_dGCh'C
Cuts at: 0 48 242 898 906
Size: 48 194 656 8
Fragments arranged by size:
656 194 48 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 102 906
Size: 102 804
BsrDI GCAATG_nn'
Cuts at: 0 414 906
Size: 414 492
BsrGI T'GTAC_A
Cuts at: 0 344 906
Size: 344 562
BssSI C'ACGA_G
Cuts at: 0 747 906
Size: 747 159
BstAPI GCAn_nnn'nTGC
Cuts at: 0 668 906
Size: 668 238
Bsu36I CC'TnA_GG
Cuts at: 0 452 906
Size: 452 454
BtgI C'CryG_G
Cuts at: 0 800 906
Size: 800 106
BtsI GCAGTG_nn'
Cuts at: 0 666 722 906
Size: 666 56 184
Fragments arranged by size:
666 184 56
ClaI AT'CG_AT
Cuts at: 0 222 906
Size: 222 684
DraIII CAC_nnn'GTG
Cuts at: 0 624 906
Size: 624 282
EaeI y'GGCC_r
Cuts at: 0 35 92 101 906
Size: 35 57 9 805
Fragments arranged by size:
805 57 35 9
EarI CTCTTCn'nnn_
Cuts at: 0 28 186 906
Size: 28 158 720
Fragments arranged by size:
720 158 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 613 835 906
Size: 517 96 222 71
Fragments arranged by size:
517 222 96 71
EcoO109I rG'GnC_Cy
Cuts at: 0 44 239 327 774 906
Size: 44 195 88 447 132
Fragments arranged by size:
447 195 132 88 44
HaeII r_GCGC'y
Cuts at: 0 194 582 705 906
Size: 194 388 123 201
Fragments arranged by size:
388 201 194 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 126 158 906
Size: 126 32 748
Fragments arranged by size:
748 126 32
MscI TGG'CCA
Cuts at: 0 37 94 103 906
Size: 37 57 9 803
Fragments arranged by size:
803 57 37 9
MslI CAynn'nnrTG
Cuts at: 0 157 283 649 906
Size: 157 126 366 257
Fragments arranged by size:
366 257 157 126
MspA1I CmG'CkG
Cuts at: 0 81 267 438 891 906
Size: 81 186 171 453 15
Fragments arranged by size:
453 186 171 81 15
PpuMI rG'GwC_Cy
Cuts at: 0 327 906
Size: 327 579
PspOMI G'GGCC_C
Cuts at: 0 44 238 906
Size: 44 194 668
Fragments arranged by size:
668 194 44
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 466 498 906
Size: 466 32 408
Fragments arranged by size:
466 408 32
PstI C_TGCA'G
Cuts at: 0 167 906
Size: 167 739
PvuII CAG'CTG
Cuts at: 0 267 891 906
Size: 267 624 15
Fragments arranged by size:
624 267 15
SfcI C'TryA_G
Cuts at: 0 163 243 906
Size: 163 80 663
Fragments arranged by size:
663 163 80
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 43 100 906
Size: 43 57 806
Fragments arranged by size:
806 57 43
SmaI CCC'GGG
Cuts at: 0 373 906
Size: 373 533
StuI AGG'CCT
Cuts at: 0 52 906
Size: 52 854
StyI C'CwwG_G
Cuts at: 0 95 906
Size: 95 811
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 299 612 679 906
Size: 299 313 67 227
Fragments arranged by size:
313 299 227 67
TatI w'GTAC_w
Cuts at: 0 299 344 906
Size: 299 45 562
Fragments arranged by size:
562 299 45
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 8 40 821 853 906
Size: 8 32 781 32 53
Fragments arranged by size:
781 53 32 32 8
Tth111I GACn'n_nGTC
Cuts at: 0 682 906
Size: 682 224
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 426 579 617 906
Size: 426 153 38 289
Fragments arranged by size:
426 289 153 38
XmaI C'CCGG_G
Cuts at: 0 371 906
Size: 371 535
ZraI GAC'GTC
Cuts at: 0 363 906
Size: 363 543
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AleI AlwNI ApaI
AvaI BanII BbvCI BciVI BglI BlpI Bme1580I BmgBI
BmrI BpmI Bpu10I BsaI BsaAI BsaHI BseRI BseYI
BsgI BsmBI Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI
Bsu36I BtgI BtsI ClaI DraIII EaeI EarI Eco57MI
EcoO109I HaeII Hin4I MscI MslI MspA1I PpuMI PspOMI
PsrI PstI PvuII SfcI SfiI SmaI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AhdI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbsI BcgI BclI BfrBI BglII BmtI
BplI BpuEI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmI BspEI BspHI BsrBI BsrFI BssHII BstBI BstEII
BstXI BstYI BstZ17I BtgZI CspCI DraI DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI XmnI