Restriction map for
pF1KE0129
(Linear) (Six-base) MAPSORT of: KIEE0129.seq Check: 4506 from: 1 to: 744
KIEE0129.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 100 269 645 744
Size: 100 169 376 99
Fragments arranged by size:
376 169 100 99
AccI GT'mk_AC
Cuts at: 0 605 744
Size: 605 139
AlwNI CAG_nnn'CTG
Cuts at: 0 261 744
Size: 261 483
ApaI G_GGCC'C
Cuts at: 0 46 516 744
Size: 46 470 228
Fragments arranged by size:
470 228 46
ApaLI G'TGCA_C
Cuts at: 0 76 744
Size: 76 668
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 412 445 744
Size: 412 33 299
Fragments arranged by size:
412 299 33
BanI G'GyrC_C
Cuts at: 0 60 618 744
Size: 60 558 126
Fragments arranged by size:
558 126 60
BanII G_rGCy'C
Cuts at: 0 46 320 516 744
Size: 46 274 196 228
Fragments arranged by size:
274 228 196 46
BbeI G_GCGC'C
Cuts at: 0 64 744
Size: 64 680
BbsI GAAGACnn'nnnn_
Cuts at: 0 215 744
Size: 215 529
BglI GCCn_nnn'nGGC
Cuts at: 0 651 744
Size: 651 93
Bme1580I G_kGCm'C
Cuts at: 0 46 80 516 623 744
Size: 46 34 436 107 121
Fragments arranged by size:
436 121 107 46 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 744
Size: 439 305
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 411 744
Size: 175 236 333
Fragments arranged by size:
333 236 175
BsaHI Gr'CG_yC
Cuts at: 0 61 194 744
Size: 61 133 550
Fragments arranged by size:
550 133 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 602 632 744
Size: 602 30 112
Fragments arranged by size:
602 112 30
BseYI C'CCAG_C
Cuts at: 0 563 744
Size: 563 181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 744
Size: 385 359
BsiHKAI G_wGCw'C
Cuts at: 0 80 587 744
Size: 80 507 157
Fragments arranged by size:
507 157 80
Bsp1286I G_dGCh'C
Cuts at: 0 46 80 320 516 587 623 744
Size: 46 34 240 196 71 36 121
Fragments arranged by size:
240 196 121 71 46 36 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 100 269 440 591 645 744
Size: 100 169 171 151 54 99
Fragments arranged by size:
171 169 151 100 99 54
BsrDI GCAATG_nn'
Cuts at: 0 140 744
Size: 140 604
BsrFI r'CCGG_y
Cuts at: 0 57 238 744
Size: 57 181 506
Fragments arranged by size:
506 181 57
BssSI C'ACGA_G
Cuts at: 0 269 744
Size: 269 475
BstAPI GCAn_nnn'nTGC
Cuts at: 0 261 744
Size: 261 483
BstYI r'GATC_y
Cuts at: 0 737 744
Size: 737 7
BtgI C'CryG_G
Cuts at: 0 534 575 646 725 744
Size: 534 41 71 79 19
Fragments arranged by size:
534 79 71 41 19
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 555 744
Size: 555 189
BtsI GCAGTG_nn'
Cuts at: 0 146 373 744
Size: 146 227 371
Fragments arranged by size:
371 227 146
EaeI y'GGCC_r
Cuts at: 0 236 744
Size: 236 508
EarI CTCTTCn'nnn_
Cuts at: 0 245 744
Size: 245 499
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 35 744
Size: 35 709
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 744
Size: 439 305
EcoO109I rG'GnC_Cy
Cuts at: 0 28 42 513 744
Size: 28 14 471 231
Fragments arranged by size:
471 231 28 14
HaeII r_GCGC'y
Cuts at: 0 64 544 744
Size: 64 480 200
Fragments arranged by size:
480 200 64
KasI G'GCGC_C
Cuts at: 0 60 744
Size: 60 684
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 744
Size: 314 430
MslI CAynn'nnrTG
Cuts at: 0 283 574 744
Size: 283 291 170
Fragments arranged by size:
291 283 170
MspA1I CmG'CkG
Cuts at: 0 479 486 744
Size: 479 7 258
Fragments arranged by size:
479 258 7
NaeI GCC'GGC
Cuts at: 0 59 240 744
Size: 59 181 504
Fragments arranged by size:
504 181 59
NarI GG'CG_CC
Cuts at: 0 61 744
Size: 61 683
NcoI C'CATG_G
Cuts at: 0 534 725 744
Size: 534 191 19
Fragments arranged by size:
534 191 19
NgoMIV G'CCGG_C
Cuts at: 0 57 238 744
Size: 57 181 506
Fragments arranged by size:
506 181 57
PflMI CCAn_nnn'nTGG
Cuts at: 0 444 744
Size: 444 300
PfoI T'CCnGG_A
Cuts at: 0 350 744
Size: 350 394
PspOMI G'GGCC_C
Cuts at: 0 42 512 744
Size: 42 470 232
Fragments arranged by size:
470 232 42
PstI C_TGCA'G
Cuts at: 0 36 602 744
Size: 36 566 142
Fragments arranged by size:
566 142 36
PvuII CAG'CTG
Cuts at: 0 479 744
Size: 479 265
SapI GCTCTTCn'nnn_
Cuts at: 0 245 744
Size: 245 499
SbfI CC_TGCA'GG
Cuts at: 0 602 744
Size: 602 142
SexAI A'CCwGG_T
Cuts at: 0 443 744
Size: 443 301
SfcI C'TryA_G
Cuts at: 0 32 598 744
Size: 32 566 146
Fragments arranged by size:
566 146 32
SfoI GGC'GCC
Cuts at: 0 62 744
Size: 62 682
SspI AAT'ATT
Cuts at: 0 696 744
Size: 696 48
StyI C'CwwG_G
Cuts at: 0 534 725 744
Size: 534 191 19
Fragments arranged by size:
534 191 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 602 634 744
Size: 602 32 110
Fragments arranged by size:
602 110 32
Enzymes that do cut:
AarI AccI AlwNI ApaI ApaLI BaeI BanI BanII
BbeI BbsI BglI Bme1580I BpmI BsaI BsaHI BsaXI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssSI
BstAPI BstYI BtgI BtgZI BtsI EaeI EarI EciI
Eco57MI EcoO109I HaeII KasI MmeI MslI MspA1I NaeI
NarI NcoI NgoMIV PflMI PfoI PspOMI PstI PvuII
SapI SbfI SexAI SfcI SfoI SspI StyI TstI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvaI AvrII BamHI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI
BsaAI BsaBI BsaWI BseRI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII BstXI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI ScaI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI