(Linear) (Six-base) MAPSORT of: KIEE0126.seq  Check: 5139  from: 1  to: 375



KIEE0126.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     102     135     375 
   Size:        102      33     240

  Fragments arranged by size: 

                 240     102      33
 
BanII G_rGCy'C

Cuts at:      0     188     375 
   Size:        188     187
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      19     375 
   Size:         19     356
 
BsiHKAI G_wGCw'C

Cuts at:      0     188     375 
   Size:        188     187
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     276     343     375 
   Size:        276      67      32

  Fragments arranged by size: 

                 276      67      32
 
Bsp1286I G_dGCh'C

Cuts at:      0     188     375 
   Size:        188     187
 
BsrGI T'GTAC_A

Cuts at:      0     326     375 
   Size:        326      49
 
BstBI TT'CG_AA

Cuts at:      0      38     375 
   Size:         38     337
 
EcoICRI GAG'CTC

Cuts at:      0     186     375 
   Size:        186     189
 
HaeII r_GCGC'y

Cuts at:      0      30     210     375 
   Size:         30     180     165

  Fragments arranged by size: 

                 180     165      30
 
HincII GTy'rAC

Cuts at:      0     174     375 
   Size:        174     201
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      84     106     375 
   Size:         84      22     269

  Fragments arranged by size: 

                 269      84      22
 
SacI G_AGCT'C

Cuts at:      0     188     375 
   Size:        188     187
 
StuI AGG'CCT

Cuts at:      0     232     375 
   Size:        232     143
 
TatI w'GTAC_w

Cuts at:      0     326     375 
   Size:        326      49
 
Tth111I GACn'n_nGTC

Cuts at:      0     169     375 
   Size:        169     206
 
XmnI GAAnn'nnTTC

Cuts at:      0      16     375 
   Size:         16     359
 

 Enzymes that do cut:

     BaeI    BanII     BbsI  BsiHKAI    BsmBI Bsp1286I    BsrGI    BstBI
  EcoICRI    HaeII   HincII     MmeI     SacI     StuI     TatI  Tth111I
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI
     BanI     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI    TaqII     TstI     XbaI     XcmI
     XhoI     XmaI     ZraI