(Linear) (Six-base) MAPSORT of: KIEE0125.seq  Check: 8065  from: 1  to: 363



KIEE0125.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AccI GT'mk_AC

Cuts at:      0     335     363 
   Size:        335      28
 
AfeI AGC'GCT

Cuts at:      0     109     363 
   Size:        109     254
 
BanI G'GyrC_C

Cuts at:      0     203     363 
   Size:        203     160
 
BbeI G_GCGC'C

Cuts at:      0     207     363 
   Size:        207     156
 
BclI T'GATC_A

Cuts at:      0      98     363 
   Size:         98     265
 
BsaHI Gr'CG_yC

Cuts at:      0     204     363 
   Size:        204     159
 
BseYI C'CCAG_C

Cuts at:      0      82     363 
   Size:         82     281
 
BsiEI CG_ry'CG

Cuts at:      0      45     363 
   Size:         45     318
 
BsrFI r'CCGG_y

Cuts at:      0      11     363 
   Size:         11     352
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     156     363 
   Size:        156     207
 
BstYI r'GATC_y

Cuts at:      0      65     363 
   Size:         65     298
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     267     363 
   Size:        267      96
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      45     363 
   Size:         45     318
 
HaeII r_GCGC'y

Cuts at:      0     111     207     363 
   Size:        111      96     156

  Fragments arranged by size: 

                 156     111      96
 
HincII GTy'rAC

Cuts at:      0     174     363 
   Size:        174     189
 
KasI G'GCGC_C

Cuts at:      0     203     363 
   Size:        203     160
 
MspA1I CmG'CkG

Cuts at:      0      27      47     129     216     363 
   Size:         27      20      82      87     147

  Fragments arranged by size: 

                 147      87      82      27      20
 
NarI GG'CG_CC

Cuts at:      0     204     363 
   Size:        204     159
 
PfoI T'CCnGG_A

Cuts at:      0     137     161     363 
   Size:        137      24     202

  Fragments arranged by size: 

                 202     137      24
 
PstI C_TGCA'G

Cuts at:      0     215     363 
   Size:        215     148
 
PvuII CAG'CTG

Cuts at:      0     129     216     363 
   Size:        129      87     147

  Fragments arranged by size: 

                 147     129      87
 
SfcI C'TryA_G

Cuts at:      0     211     363 
   Size:        211     152
 
SfoI GGC'GCC

Cuts at:      0     205     363 
   Size:        205     158
 
StuI AGG'CCT

Cuts at:      0     232     363 
   Size:        232     131
 
Tth111I GACn'n_nGTC

Cuts at:      0     169     363 
   Size:        169     194
 

 Enzymes that do cut:

     AccI     AfeI     BanI     BbeI     BclI    BsaHI    BseYI    BsiEI
    BsrFI    BstXI    BstYI    BtgZI     EciI    HaeII   HincII     KasI
   MspA1I     NarI     PfoI     PstI    PvuII     SfcI     SfoI     StuI
  Tth111I

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI    TaqII     TatI     TstI     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI