(Linear) (Six-base) MAPSORT of: KIEE0124.seq  Check: 5786  from: 1  to: 981



KIEE0124.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     598     981 
   Size:        598     383
 
AclI AA'CG_TT

Cuts at:      0     386     981 
   Size:        386     595
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     881     981 
   Size:        881     100
 
AleI CACnn'nnGTG

Cuts at:      0     229     981 
   Size:        229     752
 
ApoI r'AATT_y

Cuts at:      0     313     466     981 
   Size:        313     153     515

  Fragments arranged by size: 

                 515     313     153
 
BamHI G'GATC_C

Cuts at:      0     643     981 
   Size:        643     338
 
BglII A'GATC_T

Cuts at:      0     560     981 
   Size:        560     421
 
BlpI GC'TnA_GC

Cuts at:      0     693     981 
   Size:        693     288
 
BsaWI w'CCGG_w

Cuts at:      0      86     981 
   Size:         86     895
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     530     952     981 
   Size:        530     422      29

  Fragments arranged by size: 

                 530     422      29
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     632     889     981 
   Size:        632     257      92

  Fragments arranged by size: 

                 632     257      92
 
BspEI T'CCGG_A

Cuts at:      0      86     981 
   Size:         86     895
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     578     598     717     981 
   Size:        578      20     119     264

  Fragments arranged by size: 

                 578     264     119      20
 
BsrBI CCG'CTC

Cuts at:      0     253     981 
   Size:        253     728
 
BsrDI GCAATG_nn'

Cuts at:      0     188     981 
   Size:        188     793
 
BssSI C'ACGA_G

Cuts at:      0     865     907     981 
   Size:        865      42      74

  Fragments arranged by size: 

                 865      74      42
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      99     981 
   Size:         99     882
 
BstYI r'GATC_y

Cuts at:      0     560     643     981 
   Size:        560      83     338

  Fragments arranged by size: 

                 560     338      83
 
DraI TTT'AAA

Cuts at:      0     218     354     981 
   Size:        218     136     627

  Fragments arranged by size: 

                 627     218     136
 
DraIII CAC_nnn'GTG

Cuts at:      0      99     981 
   Size:         99     882
 
EarI CTCTTCn'nnn_

Cuts at:      0     286     917     968     981 
   Size:        286     631      51      13

  Fragments arranged by size: 

                 631     286      51      13
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     881     981 
   Size:        881     100
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     698     981 
   Size:        698     283
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     170     196     202     228     981 
   Size:        170      26       6      26     753

  Fragments arranged by size: 

                 753     170      26      26       6
 
MslI CAynn'nnrTG

Cuts at:      0     229     274     981 
   Size:        229      45     707

  Fragments arranged by size: 

                 707     229      45
 
NspI r_CATG'y

Cuts at:      0     279     981 
   Size:        279     702
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     270     981 
   Size:        270     711
 
PfoI T'CCnGG_A

Cuts at:      0      56     431     981 
   Size:         56     375     550

  Fragments arranged by size: 

                 550     375      56
 
SapI GCTCTTCn'nnn_

Cuts at:      0     286     917     981 
   Size:        286     631      64

  Fragments arranged by size: 

                 631     286      64
 
SfcI C'TryA_G

Cuts at:      0     117     506     981 
   Size:        117     389     475

  Fragments arranged by size: 

                 475     389     117
 
XmnI GAAnn'nnTTC

Cuts at:      0     341     981 
   Size:        341     640
 

 Enzymes that do cut:

     AarI     AclI     AcuI     AleI     ApoI    BamHI    BglII     BlpI
    BsaWI    BseRI     BsgI    BspEI    BspMI    BsrBI    BsrDI    BssSI
   BstAPI    BstYI     DraI   DraIII     EarI  Eco57MI     FalI    Hin4I
     MslI     NspI    PflMI     PfoI     SapI     SfcI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI Bme1580I    BmgBI     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspHI    BsrFI    BsrGI   BssHII    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI