Restriction map for
pF1KE0123
(Linear) (Six-base) MAPSORT of: KIEE0123.seq Check: 3721 from: 1 to: 867
KIEE0123.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AfeI AGC'GCT
Cuts at: 0 72 867
Size: 72 795
AlwNI CAG_nnn'CTG
Cuts at: 0 783 867
Size: 783 84
ApaI G_GGCC'C
Cuts at: 0 762 792 867
Size: 762 30 75
Fragments arranged by size:
762 75 30
AvaI C'yCGr_G
Cuts at: 0 110 439 563 867
Size: 110 329 124 304
Fragments arranged by size:
329 304 124 110
BamHI G'GATC_C
Cuts at: 0 474 867
Size: 474 393
BanI G'GyrC_C
Cuts at: 0 805 867
Size: 805 62
BanII G_rGCy'C
Cuts at: 0 440 762 792 867
Size: 440 322 30 75
Fragments arranged by size:
440 322 75 30
BciVI GTATCCnnnnn_n'
Cuts at: 0 288 867
Size: 288 579
Bme1580I G_kGCm'C
Cuts at: 0 762 792 867
Size: 762 30 75
Fragments arranged by size:
762 75 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 736 867
Size: 89 647 131
Fragments arranged by size:
647 131 89
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 625 867
Size: 625 242
BsaHI Gr'CG_yC
Cuts at: 0 36 867
Size: 36 831
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 26 56 709 739 867
Size: 26 30 653 30 128
Fragments arranged by size:
653 128 30 30 26
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 448 733 736 867
Size: 21 427 285 3 131
Fragments arranged by size:
427 285 131 21 3
BseYI C'CCAG_C
Cuts at: 0 67 244 287 544 635 867
Size: 67 177 43 257 91 232
Fragments arranged by size:
257 232 177 91 67 43
BsiEI CG_ry'CG
Cuts at: 0 55 867
Size: 55 812
BsiHKAI G_wGCw'C
Cuts at: 0 575 838 867
Size: 575 263 29
Fragments arranged by size:
575 263 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 46 107 867
Size: 46 61 760
Fragments arranged by size:
760 61 46
Bsp1286I G_dGCh'C
Cuts at: 0 440 575 762 792 838 867
Size: 440 135 187 30 46 29
Fragments arranged by size:
440 187 135 46 30 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 683 867
Size: 683 184
BsrDI GCAATG_nn'
Cuts at: 0 260 867
Size: 260 607
BsrFI r'CCGG_y
Cuts at: 0 54 802 867
Size: 54 748 65
Fragments arranged by size:
748 65 54
BstAPI GCAn_nnn'nTGC
Cuts at: 0 699 783 867
Size: 699 84 84
Fragments arranged by size:
699 84 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 267 537 867
Size: 267 270 330
Fragments arranged by size:
330 270 267
BstYI r'GATC_y
Cuts at: 0 474 731 867
Size: 474 257 136
Fragments arranged by size:
474 257 136
BtgI C'CryG_G
Cuts at: 0 647 867
Size: 647 220
BtsI GCAGTG_nn'
Cuts at: 0 272 867
Size: 272 595
DraIII CAC_nnn'GTG
Cuts at: 0 172 332 867
Size: 172 160 535
Fragments arranged by size:
535 172 160
EaeI y'GGCC_r
Cuts at: 0 52 257 867
Size: 52 205 610
Fragments arranged by size:
610 205 52
EagI C'GGCC_G
Cuts at: 0 52 867
Size: 52 815
EarI CTCTTCn'nnn_
Cuts at: 0 133 817 867
Size: 133 684 50
Fragments arranged by size:
684 133 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 736 867
Size: 89 647 131
Fragments arranged by size:
647 131 89
EcoNI CCTnn'n_nnAGG
Cuts at: 0 718 867
Size: 718 149
EcoO109I rG'GnC_Cy
Cuts at: 0 252 541 867
Size: 252 289 326
Fragments arranged by size:
326 289 252
HaeII r_GCGC'y
Cuts at: 0 74 867
Size: 74 793
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 58 132 164 533 565 867
Size: 26 32 74 32 369 32 302
Fragments arranged by size:
369 302 74 32 32 32 26
MscI TGG'CCA
Cuts at: 0 259 867
Size: 259 608
MslI CAynn'nnrTG
Cuts at: 0 265 767 867
Size: 265 502 100
Fragments arranged by size:
502 265 100
MspA1I CmG'CkG
Cuts at: 0 378 867
Size: 378 489
NaeI GCC'GGC
Cuts at: 0 56 804 867
Size: 56 748 63
Fragments arranged by size:
748 63 56
NcoI C'CATG_G
Cuts at: 0 647 867
Size: 647 220
NgoMIV G'CCGG_C
Cuts at: 0 54 802 867
Size: 54 748 65
Fragments arranged by size:
748 65 54
PpuMI rG'GwC_Cy
Cuts at: 0 541 867
Size: 541 326
PspOMI G'GGCC_C
Cuts at: 0 758 788 867
Size: 758 30 79
Fragments arranged by size:
758 79 30
PstI C_TGCA'G
Cuts at: 0 779 867
Size: 779 88
PvuII CAG'CTG
Cuts at: 0 378 867
Size: 378 489
SanDI GG'GwC_CC
Cuts at: 0 541 867
Size: 541 326
ScaI AGT'ACT
Cuts at: 0 307 867
Size: 307 560
SfcI C'TryA_G
Cuts at: 0 423 775 867
Size: 423 352 92
Fragments arranged by size:
423 352 92
SmaI CCC'GGG
Cuts at: 0 112 867
Size: 112 755
SmlI C'TyrA_G
Cuts at: 0 640 867
Size: 640 227
SrfI GCCC'GGGC
Cuts at: 0 112 867
Size: 112 755
StyI C'CwwG_G
Cuts at: 0 647 659 867
Size: 647 12 208
Fragments arranged by size:
647 208 12
TatI w'GTAC_w
Cuts at: 0 305 867
Size: 305 562
Tth111I GACn'n_nGTC
Cuts at: 0 38 867
Size: 38 829
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 485 534 867
Size: 252 233 49 333
Fragments arranged by size:
333 252 233 49
XmaI C'CCGG_G
Cuts at: 0 110 867
Size: 110 757
Enzymes that do cut:
AfeI AlwNI ApaI AvaI BamHI BanI BanII BciVI
Bme1580I BpmI BpuEI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI
BstYI BtgI BtsI DraIII EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I MscI MslI MspA1I NaeI
NcoI NgoMIV PpuMI PspOMI PstI PvuII SanDI ScaI
SfcI SmaI SmlI SrfI StyI TatI Tth111I XcmI
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaWI BsgI BsiWI
BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SspI StuI SwaI TaqII TstI XbaI
XhoI XmnI ZraI