(Linear) (Six-base) MAPSORT of: KIEE0117.seq  Check: 535  from: 1  to: 639



KIEE0117.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:10  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     584     639 
   Size:        584      55
 
AflIII A'CryG_T

Cuts at:      0     327     639 
   Size:        327     312
 
AleI CACnn'nnGTG

Cuts at:      0     371     639 
   Size:        371     268
 
AseI AT'TA_AT

Cuts at:      0     473     639 
   Size:        473     166
 
AvaI C'yCGr_G

Cuts at:      0     461     639 
   Size:        461     178
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     247     639 
   Size:        247     392
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     382     416     639 
   Size:        382      34     223

  Fragments arranged by size: 

                 382     223      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     183     375     639 
   Size:        183     192     264

  Fragments arranged by size: 

                 264     192     183
 
BglII A'GATC_T

Cuts at:      0     182     639 
   Size:        182     457
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     639 
   Size:        163     476
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     310     639 
   Size:        310     329
 
BsaBI GATnn'nnATC

Cuts at:      0     541     639 
   Size:        541      98
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     639 
   Size:        639
 
BseYI C'CCAG_C

Cuts at:      0     554     639 
   Size:        554      85
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     272     639 
   Size:        272     367
 
BssSI C'ACGA_G

Cuts at:      0     406     639 
   Size:        406     233
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      65     639 
   Size:         65     574
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     477     639 
   Size:        477     162
 
BstYI r'GATC_y

Cuts at:      0     182     214     639 
   Size:        182      32     425

  Fragments arranged by size: 

                 425     182      32
 
BtgI C'CryG_G

Cuts at:      0     119     194     639 
   Size:        119      75     445

  Fragments arranged by size: 

                 445     119      75
 
EarI CTCTTCn'nnn_

Cuts at:      0     308     562     599     603     639 
   Size:        308     254      37       4      36

  Fragments arranged by size: 

                 308     254      37      36       4
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      64     639 
   Size:         64     575
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     584     639 
   Size:        163     421      55

  Fragments arranged by size: 

                 421     163      55
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     234     639 
   Size:        234     405
 
EcoO109I rG'GnC_Cy

Cuts at:      0     284     639 
   Size:        284     355
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     121     153     639 
   Size:        121      32     486

  Fragments arranged by size: 

                 486     121      32
 
MslI CAynn'nnrTG

Cuts at:      0     371     475     639 
   Size:        371     104     164

  Fragments arranged by size: 

                 371     164     104
 
MspA1I CmG'CkG

Cuts at:      0     196     639 
   Size:        196     443
 
PfoI T'CCnGG_A

Cuts at:      0     545     639 
   Size:        545      94
 
PpuMI rG'GwC_Cy

Cuts at:      0     284     639 
   Size:        284     355
 
SacII CC_GC'GG

Cuts at:      0     197     639 
   Size:        197     442
 
SfcI C'TryA_G

Cuts at:      0     234     639 
   Size:        234     405
 
TatI w'GTAC_w

Cuts at:      0     324     443     639 
   Size:        324     119     196

  Fragments arranged by size: 

                 324     196     119
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     123     639 
   Size:        123     516
 
XmnI GAAnn'nnTTC

Cuts at:      0     487     639 
   Size:        487     152
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     AseI     AvaI     BbsI     BcgI    BciVI
    BglII     BpmI     BsaI    BsaBI    BseRI    BseYI    BspMI    BssSI
   BstAPI    BstXI    BstYI     BtgI     EarI     EciI  Eco57MI    EcoNI
 EcoO109I    Hin4I     MslI   MspA1I     PfoI    PpuMI    SacII     SfcI
     TatI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI    BbvCI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI    TaqII     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI