Restriction map for
pF1KE0110
(Linear) (Six-base) MAPSORT of: KIEE0110.seq Check: 3449 from: 1 to: 777
KIEE0110.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 777
Size: 224 553
AleI CACnn'nnGTG
Cuts at: 0 526 777
Size: 526 251
ApoI r'AATT_y
Cuts at: 0 190 374 777
Size: 190 184 403
Fragments arranged by size:
403 190 184
AseI AT'TA_AT
Cuts at: 0 707 777
Size: 707 70
BanI G'GyrC_C
Cuts at: 0 351 777
Size: 351 426
BanII G_rGCy'C
Cuts at: 0 639 777
Size: 639 138
BbsI GAAGACnn'nnnn_
Cuts at: 0 550 777
Size: 550 227
BglII A'GATC_T
Cuts at: 0 36 777
Size: 36 741
BlpI GC'TnA_GC
Cuts at: 0 462 777
Size: 462 315
Bme1580I G_kGCm'C
Cuts at: 0 294 574 777
Size: 294 280 203
Fragments arranged by size:
294 280 203
BmrI ACTGGGnnnn_n'
Cuts at: 0 683 777
Size: 683 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 758 777
Size: 758 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 777
Size: 623 154
BsaAI yAC'GTr
Cuts at: 0 106 777
Size: 106 671
BsaBI GATnn'nnATC
Cuts at: 0 424 777
Size: 424 353
BsaWI w'CCGG_w
Cuts at: 0 392 777
Size: 392 385
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 604 777
Size: 604 173
BseYI C'CCAG_C
Cuts at: 0 654 777
Size: 654 123
BsiHKAI G_wGCw'C
Cuts at: 0 414 777
Size: 414 363
BsmI GAATG_Cn'
Cuts at: 0 388 777
Size: 388 389
Bsp1286I G_dGCh'C
Cuts at: 0 294 414 574 639 777
Size: 294 120 160 65 138
Fragments arranged by size:
294 160 138 120 65
BspHI T'CATG_A
Cuts at: 0 599 777
Size: 599 178
BsrBI CCG'CTC
Cuts at: 0 252 777
Size: 252 525
BsrDI GCAATG_nn'
Cuts at: 0 562 777
Size: 562 215
BstAPI GCAn_nnn'nTGC
Cuts at: 0 13 777
Size: 13 764
BstXI CCAn_nnnn'nTGG
Cuts at: 0 528 777
Size: 528 249
BstYI r'GATC_y
Cuts at: 0 36 777
Size: 36 741
Bsu36I CC'TnA_GG
Cuts at: 0 587 777
Size: 587 190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 777
Size: 224 553
EcoO109I rG'GnC_Cy
Cuts at: 0 472 777
Size: 472 305
EcoRV GAT'ATC
Cuts at: 0 198 777
Size: 198 579
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 567 777
Size: 567 210
MslI CAynn'nnrTG
Cuts at: 0 526 777
Size: 526 251
MspA1I CmG'CkG
Cuts at: 0 70 777
Size: 70 707
NspI r_CATG'y
Cuts at: 0 11 654 777
Size: 11 643 123
Fragments arranged by size:
643 123 11
PasI CC'CwG_GG
Cuts at: 0 468 777
Size: 468 309
PfoI T'CCnGG_A
Cuts at: 0 401 777
Size: 401 376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 532 564 777
Size: 532 32 213
Fragments arranged by size:
532 213 32
PstI C_TGCA'G
Cuts at: 0 234 767 777
Size: 234 533 10
Fragments arranged by size:
533 234 10
PvuII CAG'CTG
Cuts at: 0 70 777
Size: 70 707
SfcI C'TryA_G
Cuts at: 0 230 763 777
Size: 230 533 14
Fragments arranged by size:
533 230 14
SmlI C'TyrA_G
Cuts at: 0 638 777
Size: 638 139
SphI G_CATG'C
Cuts at: 0 11 654 777
Size: 11 643 123
Fragments arranged by size:
643 123 11
SspI AAT'ATT
Cuts at: 0 215 777
Size: 215 562
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 470 502 777
Size: 470 32 275
Fragments arranged by size:
470 275 32
XmnI GAAnn'nnTTC
Cuts at: 0 362 547 777
Size: 362 185 230
Fragments arranged by size:
362 230 185
Enzymes that do cut:
AcuI AleI ApoI AseI BanI BanII BbsI BglII
BlpI Bme1580I BmrI BplI BpuEI BsaAI BsaBI BsaWI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrDI
BstAPI BstXI BstYI Bsu36I Eco57MI EcoO109I EcoRV FalI
MslI MspA1I NspI PasI PfoI PpiI PstI PvuII
SfcI SmlI SphI SspI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BmgBI BmtI BpmI Bpu10I
BsaI BsaHI BsaXI BsgI BsiEI BsiWI BsmBI BspEI
BspMI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoRI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StuI StyI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI ZraI