Restriction map for
pF1KE0103
(Linear) (Six-base) MAPSORT of: KIEE0103.seq Check: 565 from: 1 to: 1200
KIEE0103.orf
With 182 enzymes: SgfI *
April 24, 2009 13:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 402 1200
Size: 402 798
AccI GT'mk_AC
Cuts at: 0 599 1200
Size: 599 601
AflIII A'CryG_T
Cuts at: 0 39 1200
Size: 39 1161
AleI CACnn'nnGTG
Cuts at: 0 997 1200
Size: 997 203
ApaI G_GGCC'C
Cuts at: 0 769 1128 1200
Size: 769 359 72
Fragments arranged by size:
769 359 72
ApaLI G'TGCA_C
Cuts at: 0 265 1200
Size: 265 935
AvaI C'yCGr_G
Cuts at: 0 96 1200
Size: 96 1104
BamHI G'GATC_C
Cuts at: 0 983 1200
Size: 983 217
BanI G'GyrC_C
Cuts at: 0 277 1179 1200
Size: 277 902 21
Fragments arranged by size:
902 277 21
BanII G_rGCy'C
Cuts at: 0 119 203 769 1128 1200
Size: 119 84 566 359 72
Fragments arranged by size:
566 359 119 84 72
BbeI G_GCGC'C
Cuts at: 0 281 1200
Size: 281 919
BbsI GAAGACnn'nnnn_
Cuts at: 0 181 925 1200
Size: 181 744 275
Fragments arranged by size:
744 275 181
BbvCI CC'TCA_GC
Cuts at: 0 194 1200
Size: 194 1006
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 462 496 1200
Size: 462 34 704
Fragments arranged by size:
704 462 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 707 834 1200
Size: 707 127 366
Fragments arranged by size:
707 366 127
BglI GCCn_nnn'nGGC
Cuts at: 0 1144 1200
Size: 1144 56
BlpI GC'TnA_GC
Cuts at: 0 85 1200
Size: 85 1115
Bme1580I G_kGCm'C
Cuts at: 0 269 769 1128 1157 1200
Size: 269 500 359 29 43
Fragments arranged by size:
500 359 269 43 29
BmrI ACTGGGnnnn_n'
Cuts at: 0 503 1200
Size: 503 697
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 219 1200
Size: 219 981
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 1200
Size: 652 548
Bpu10I CC'TnA_GC
Cuts at: 0 194 616 1200
Size: 194 422 584
Fragments arranged by size:
584 422 194
BsaI GGTCTCn'nnnn_
Cuts at: 0 85 1016 1200
Size: 85 931 184
Fragments arranged by size:
931 184 85
BsaAI yAC'GTr
Cuts at: 0 354 777 1200
Size: 354 423 423
Fragments arranged by size:
423 423 354
BsaHI Gr'CG_yC
Cuts at: 0 278 1200
Size: 278 922
BsaWI w'CCGG_w
Cuts at: 0 243 1200
Size: 243 957
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 415 445 1200
Size: 415 30 755
Fragments arranged by size:
755 415 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 397 895 1120 1200
Size: 397 498 225 80
Fragments arranged by size:
498 397 225 80
BseYI C'CCAG_C
Cuts at: 0 62 768 1200
Size: 62 706 432
Fragments arranged by size:
706 432 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 1200
Size: 841 359
BsiEI CG_ry'CG
Cuts at: 0 486 1200
Size: 486 714
BsiHKAI G_wGCw'C
Cuts at: 0 269 1200
Size: 269 931
BsmBI CGTCTCn'nnnn_
Cuts at: 0 418 1200
Size: 418 782
Bsp1286I G_dGCh'C
Cuts at: 0 119 203 269 769 1128 1157 1200
Size: 119 84 66 500 359 29 43
Fragments arranged by size:
500 359 119 84 66 43 29
BspEI T'CCGG_A
Cuts at: 0 243 1200
Size: 243 957
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 402 554 1200
Size: 402 152 646
Fragments arranged by size:
646 402 152
BsrBI CCG'CTC
Cuts at: 0 746 1200
Size: 746 454
BsrDI GCAATG_nn'
Cuts at: 0 1133 1200
Size: 1133 67
BsrFI r'CCGG_y
Cuts at: 0 1138 1200
Size: 1138 62
BstEII G'GTnAC_C
Cuts at: 0 805 1200
Size: 805 395
BstXI CCAn_nnnn'nTGG
Cuts at: 0 516 522 960 1194 1200
Size: 516 6 438 234 6
Fragments arranged by size:
516 438 234 6 6
BstYI r'GATC_y
Cuts at: 0 983 1200
Size: 983 217
BtgI C'CryG_G
Cuts at: 0 515 660 861 1088 1200
Size: 515 145 201 227 112
Fragments arranged by size:
515 227 201 145 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 764 1200
Size: 764 436
BtsI GCAGTG_nn'
Cuts at: 0 527 1200
Size: 527 673
DraIII CAC_nnn'GTG
Cuts at: 0 237 1200
Size: 237 963
DrdI GACnn_nn'nnGTC
Cuts at: 0 718 814 1200
Size: 718 96 386
Fragments arranged by size:
718 386 96
EaeI y'GGCC_r
Cuts at: 0 2 495 950 1064 1200
Size: 2 493 455 114 136
Fragments arranged by size:
493 455 136 114 2
EarI CTCTTCn'nnn_
Cuts at: 0 1111 1200
Size: 1111 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 1200
Size: 652 548
EcoO109I rG'GnC_Cy
Cuts at: 0 636 927 1125 1200
Size: 636 291 198 75
Fragments arranged by size:
636 291 198 75
HaeII r_GCGC'y
Cuts at: 0 281 1200
Size: 281 919
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 26 58 1013 1045 1200
Size: 26 32 955 32 155
Fragments arranged by size:
955 155 32 32 26
HincII GTy'rAC
Cuts at: 0 600 1032 1200
Size: 600 432 168
Fragments arranged by size:
600 432 168
HindIII A'AGCT_T
Cuts at: 0 295 1200
Size: 295 905
KasI G'GCGC_C
Cuts at: 0 277 1200
Size: 277 923
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1175 1200
Size: 1175 25
MscI TGG'CCA
Cuts at: 0 497 952 1066 1200
Size: 497 455 114 134
Fragments arranged by size:
497 455 134 114
MslI CAynn'nnrTG
Cuts at: 0 520 958 997 1200
Size: 520 438 39 203
Fragments arranged by size:
520 438 203 39
MspA1I CmG'CkG
Cuts at: 0 801 940 1200
Size: 801 139 260
Fragments arranged by size:
801 260 139
NaeI GCC'GGC
Cuts at: 0 1140 1200
Size: 1140 60
NarI GG'CG_CC
Cuts at: 0 278 1200
Size: 278 922
NcoI C'CATG_G
Cuts at: 0 515 1200
Size: 515 685
NgoMIV G'CCGG_C
Cuts at: 0 1138 1200
Size: 1138 62
NspI r_CATG'y
Cuts at: 0 43 1200
Size: 43 1157
PciI A'CATG_T
Cuts at: 0 39 1200
Size: 39 1161
PflMI CCAn_nnn'nTGG
Cuts at: 0 237 316 1200
Size: 237 79 884
Fragments arranged by size:
884 237 79
PfoI T'CCnGG_A
Cuts at: 0 74 1200
Size: 74 1126
PmlI CAC'GTG
Cuts at: 0 354 777 1200
Size: 354 423 423
Fragments arranged by size:
423 423 354
PpuMI rG'GwC_Cy
Cuts at: 0 636 1200
Size: 636 564
PspOMI G'GGCC_C
Cuts at: 0 765 1124 1200
Size: 765 359 76
Fragments arranged by size:
765 359 76
PvuII CAG'CTG
Cuts at: 0 940 1200
Size: 940 260
SalI G'TCGA_C
Cuts at: 0 598 1200
Size: 598 602
SapI GCTCTTCn'nnn_
Cuts at: 0 1111 1200
Size: 1111 89
SexAI A'CCwGG_T
Cuts at: 0 1025 1200
Size: 1025 175
SfoI GGC'GCC
Cuts at: 0 279 1200
Size: 279 921
StuI AGG'CCT
Cuts at: 0 584 1200
Size: 584 616
StyI C'CwwG_G
Cuts at: 0 515 995 1200
Size: 515 480 205
Fragments arranged by size:
515 480 205
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 500 1200
Size: 500 700
TatI w'GTAC_w
Cuts at: 0 395 1200
Size: 395 805
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 248 280 1200
Size: 248 32 920
Fragments arranged by size:
920 248 32
Tth111I GACn'n_nGTC
Cuts at: 0 595 1027 1200
Size: 595 432 173
Fragments arranged by size:
595 432 173
Enzymes that do cut:
AarI AccI AflIII AleI ApaI ApaLI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI
BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstEII
BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EarI Eco57MI EcoO109I HaeII Hin4I HincII HindIII KasI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PciI PflMI PfoI PmlI PpuMI PspOMI PvuII
SalI SapI SexAI SfoI StuI StyI TaqII TatI
TstI Tth111I
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BclI BfrBI BglII BmgBI BmtI BpuEI BsaBI
BsiWI BsmI BspHI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I ClaI CspCI DraI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HpaI
KpnI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PasI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacI SacII SanDI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XcmI XhoI XmaI
XmnI ZraI