Restriction map for
pF1KE0030
(Linear) (Six-base) MAPSORT of: KIEE0030.seq Check: 7301 from: 1 to: 1656
KIEE0030.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 938 1656
Size: 938 718
AatII G_ACGT'C
Cuts at: 0 335 1656
Size: 335 1321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 503 853 1447 1516 1587 1656
Size: 25 478 350 594 69 71 69
Fragments arranged by size:
594 478 350 71 69 69 25
AfeI AGC'GCT
Cuts at: 0 826 1087 1656
Size: 826 261 569
Fragments arranged by size:
826 569 261
AflIII A'CryG_T
Cuts at: 0 935 1656
Size: 935 721
AhdI GACnn_n'nnGTC
Cuts at: 0 1173 1656
Size: 1173 483
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 940 1656
Size: 940 716
AlwNI CAG_nnn'CTG
Cuts at: 0 1632 1656
Size: 1632 24
ApaLI G'TGCA_C
Cuts at: 0 1036 1656
Size: 1036 620
AvaI C'yCGr_G
Cuts at: 0 950 1178 1235 1656
Size: 950 228 57 421
Fragments arranged by size:
950 421 228 57
BanI G'GyrC_C
Cuts at: 0 66 283 958 1656
Size: 66 217 675 698
Fragments arranged by size:
698 675 217 66
BanII G_rGCy'C
Cuts at: 0 469 1656
Size: 469 1187
BbeI G_GCGC'C
Cuts at: 0 70 287 1656
Size: 70 217 1369
Fragments arranged by size:
1369 217 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 30 842 1473 1656
Size: 30 812 631 183
Fragments arranged by size:
812 631 183 30
BbvCI CC'TCA_GC
Cuts at: 0 1393 1656
Size: 1393 263
BglI GCCn_nnn'nGGC
Cuts at: 0 1588 1656
Size: 1588 68
BlpI GC'TnA_GC
Cuts at: 0 205 1656
Size: 205 1451
Bme1580I G_kGCm'C
Cuts at: 0 326 961 1040 1656
Size: 326 635 79 616
Fragments arranged by size:
635 616 326 79
BmgBI CAC'GTC
Cuts at: 0 1065 1170 1434 1449 1656
Size: 1065 105 264 15 207
Fragments arranged by size:
1065 264 207 105 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 812 843 1009 1150 1423 1656
Size: 733 79 31 166 141 273 233
Fragments arranged by size:
733 273 233 166 141 79 31
Bpu10I CC'TnA_GC
Cuts at: 0 1393 1414 1656
Size: 1393 21 242
Fragments arranged by size:
1393 242 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 373 1656
Size: 373 1283
BsaAI yAC'GTr
Cuts at: 0 936 1587 1656
Size: 936 651 69
Fragments arranged by size:
936 651 69
BsaBI GATnn'nnATC
Cuts at: 0 1303 1656
Size: 1303 353
BsaHI Gr'CG_yC
Cuts at: 0 10 67 89 284 332 644 698 1656
Size: 10 57 22 195 48 312 54 958
Fragments arranged by size:
958 312 195 57 54 48 22 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 841 871 1656
Size: 841 30 785
Fragments arranged by size:
841 785 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 809 1600 1656
Size: 809 791 56
Fragments arranged by size:
809 791 56
BseYI C'CCAG_C
Cuts at: 0 597 672 1656
Size: 597 75 984
Fragments arranged by size:
984 597 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 910 916 1656
Size: 910 6 740
Fragments arranged by size:
910 740 6
BsiEI CG_ry'CG
Cuts at: 0 1532 1656
Size: 1532 124
BsiHKAI G_wGCw'C
Cuts at: 0 224 1040 1082 1409 1656
Size: 224 816 42 327 247
Fragments arranged by size:
816 327 247 224 42
BsmI GAATG_Cn'
Cuts at: 0 165 1515 1656
Size: 165 1350 141
Fragments arranged by size:
1350 165 141
BsmBI CGTCTCn'nnnn_
Cuts at: 0 705 985 1656
Size: 705 280 671
Fragments arranged by size:
705 671 280
Bsp1286I G_dGCh'C
Cuts at: 0 224 326 469 961 1040 1082 1409 1656
Size: 224 102 143 492 79 42 327 247
Fragments arranged by size:
492 327 247 224 143 102 79 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 140 938 1550 1656
Size: 140 798 612 106
Fragments arranged by size:
798 612 140 106
BsrBI CCG'CTC
Cuts at: 0 695 1656
Size: 695 961
BsrDI GCAATG_nn'
Cuts at: 0 1217 1559 1656
Size: 1217 342 97
Fragments arranged by size:
1217 342 97
BsrFI r'CCGG_y
Cuts at: 0 1281 1527 1656
Size: 1281 246 129
Fragments arranged by size:
1281 246 129
BssHII G'CGCG_C
Cuts at: 0 181 1656
Size: 181 1475
BssSI C'ACGA_G
Cuts at: 0 97 1081 1209 1636 1656
Size: 97 984 128 427 20
Fragments arranged by size:
984 427 128 97 20
BstEII G'GTnAC_C
Cuts at: 0 525 1656
Size: 525 1131
BstXI CCAn_nnnn'nTGG
Cuts at: 0 312 1656
Size: 312 1344
BstYI r'GATC_y
Cuts at: 0 1304 1656
Size: 1304 352
Bsu36I CC'TnA_GG
Cuts at: 0 1016 1656
Size: 1016 640
BtgI C'CryG_G
Cuts at: 0 287 311 374 404 905 1031 1354 1656
Size: 287 24 63 30 501 126 323 302
Fragments arranged by size:
501 323 302 287 126 63 30 24
BtsI GCAGTG_nn'
Cuts at: 0 170 503 1656
Size: 170 333 1153
Fragments arranged by size:
1153 333 170
EaeI y'GGCC_r
Cuts at: 0 314 371 500 998 1529 1656
Size: 314 57 129 498 531 127
Fragments arranged by size:
531 498 314 129 127 57
EagI C'GGCC_G
Cuts at: 0 1529 1656
Size: 1529 127
EarI CTCTTCn'nnn_
Cuts at: 0 733 1656
Size: 733 923
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 211 444 459 617 1072 1087 1656
Size: 211 233 15 158 455 15 569
Fragments arranged by size:
569 455 233 211 158 15 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 503 733 812 843 853 1009 1150
Size: 25 478 230 79 31 10 156 141
Cuts at: 1150 1423 1447 1516 1587 1656
Size: 273 24 69 71 69
Fragments arranged by size:
478 273 230 156 141 79 71 69
69 31 25 24 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 868 1057 1656
Size: 868 189 599
Fragments arranged by size:
868 599 189
EcoO109I rG'GnC_Cy
Cuts at: 0 264 417 609 1227 1467 1656
Size: 264 153 192 618 240 189
Fragments arranged by size:
618 264 240 192 189 153
FspI TGC'GCA
Cuts at: 0 496 1656
Size: 496 1160
HaeII r_GCGC'y
Cuts at: 0 70 287 828 1089 1656
Size: 70 217 541 261 567
Fragments arranged by size:
567 541 261 217 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1159 1191 1656
Size: 1159 32 465
Fragments arranged by size:
1159 465 32
KasI G'GCGC_C
Cuts at: 0 66 283 1656
Size: 66 217 1373
Fragments arranged by size:
1373 217 66
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 710 1656
Size: 710 946
MscI TGG'CCA
Cuts at: 0 373 1656
Size: 373 1283
MslI CAynn'nnrTG
Cuts at: 0 1168 1282 1656
Size: 1168 114 374
Fragments arranged by size:
1168 374 114
MspA1I CmG'CkG
Cuts at: 0 49 75 215 533 1320 1356 1368 1494
Size: 49 26 140 318 787 36 12 126
Cuts at: 1494 1656
Size: 162
Fragments arranged by size:
787 318 162 140 126 49 36 26
12
NaeI GCC'GGC
Cuts at: 0 1529 1656
Size: 1529 127
NarI GG'CG_CC
Cuts at: 0 67 284 1656
Size: 67 217 1372
Fragments arranged by size:
1372 217 67
NcoI C'CATG_G
Cuts at: 0 311 374 1031 1656
Size: 311 63 657 625
Fragments arranged by size:
657 625 311 63
NgoMIV G'CCGG_C
Cuts at: 0 1527 1656
Size: 1527 129
NspI r_CATG'y
Cuts at: 0 120 1281 1656
Size: 120 1161 375
Fragments arranged by size:
1161 375 120
PasI CC'CwG_GG
Cuts at: 0 305 462 1656
Size: 305 157 1194
Fragments arranged by size:
1194 305 157
PflMI CCAn_nnn'nTGG
Cuts at: 0 827 1656
Size: 827 829
PmlI CAC'GTG
Cuts at: 0 936 1656
Size: 936 720
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1009 1041 1656
Size: 1009 32 615
Fragments arranged by size:
1009 615 32
PpuMI rG'GwC_Cy
Cuts at: 0 609 1467 1656
Size: 609 858 189
Fragments arranged by size:
858 609 189
PshAI GACnn'nnGTC
Cuts at: 0 385 1237 1656
Size: 385 852 419
Fragments arranged by size:
852 419 385
PspXI vC'TCGA_Gb
Cuts at: 0 1235 1656
Size: 1235 421
PstI C_TGCA'G
Cuts at: 0 137 968 1656
Size: 137 831 688
Fragments arranged by size:
831 688 137
PvuII CAG'CTG
Cuts at: 0 1320 1368 1494 1656
Size: 1320 48 126 162
Fragments arranged by size:
1320 162 126 48
SacII CC_GC'GG
Cuts at: 0 1357 1656
Size: 1357 299
SanDI GG'GwC_CC
Cuts at: 0 1467 1656
Size: 1467 189
SbfI CC_TGCA'GG
Cuts at: 0 137 1656
Size: 137 1519
ScaI AGT'ACT
Cuts at: 0 488 1656
Size: 488 1168
SfcI C'TryA_G
Cuts at: 0 133 964 1656
Size: 133 831 692
Fragments arranged by size:
831 692 133
SfoI GGC'GCC
Cuts at: 0 68 285 1656
Size: 68 217 1371
Fragments arranged by size:
1371 217 68
SmaI CCC'GGG
Cuts at: 0 1180 1656
Size: 1180 476
SmlI C'TyrA_G
Cuts at: 0 1235 1656
Size: 1235 421
SphI G_CATG'C
Cuts at: 0 120 1656
Size: 120 1536
StyI C'CwwG_G
Cuts at: 0 311 374 1031 1656
Size: 311 63 657 625
Fragments arranged by size:
657 625 311 63
TatI w'GTAC_w
Cuts at: 0 395 486 1656
Size: 395 91 1170
Fragments arranged by size:
1170 395 91
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 841 873 1656
Size: 841 32 783
Fragments arranged by size:
841 783 32
Tth111I GACn'n_nGTC
Cuts at: 0 334 1656
Size: 334 1322
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 627 828 1656
Size: 627 201 828
Fragments arranged by size:
828 627 201
XhoI C'TCGA_G
Cuts at: 0 1235 1656
Size: 1235 421
XmaI C'CCGG_G
Cuts at: 0 1178 1656
Size: 1178 478
ZraI GAC'GTC
Cuts at: 0 333 1656
Size: 333 1323
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AlfI AlwNI
ApaLI AvaI BanI BanII BbeI BbsI BbvCI BglI
BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PflMI PmlI PpiI PpuMI PshAI
PspXI PstI PvuII SacII SanDI SbfI ScaI SfcI
SfoI SmaI SmlI SphI StyI TatI TstI Tth111I
XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AleI AloI ApaI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BglII BmrI BmtI BplI BpuEI
BsaWI BsiWI BspEI BspHI BsrGI BstAPI BstBI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PsiI PspOMI PsrI PvuI RsrII
SacI SalI SapI SexAI SfiI SgfI SgrAI SnaBI
SpeI SrfI SspI StuI SwaI TaqII XbaI XmnI