Restriction map for
pF1KE0027
(Linear) (Six-base) MAPSORT of: KIEE0027.seq Check: 8715 from: 1 to: 1710
KIEE0027.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AatII G_ACGT'C
Cuts at: 0 146 1710
Size: 146 1564
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 1710
Size: 235 1475
AflIII A'CryG_T
Cuts at: 0 1225 1710
Size: 1225 485
AleI CACnn'nnGTG
Cuts at: 0 986 1710
Size: 986 724
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 104 887 980 1710
Size: 104 783 93 730
Fragments arranged by size:
783 730 104 93
AlwNI CAG_nnn'CTG
Cuts at: 0 488 690 1021 1557 1710
Size: 488 202 331 536 153
Fragments arranged by size:
536 488 331 202 153
ApaI G_GGCC'C
Cuts at: 0 15 1710
Size: 15 1695
ApaLI G'TGCA_C
Cuts at: 0 862 1710
Size: 862 848
AvaI C'yCGr_G
Cuts at: 0 14 123 928 1710
Size: 14 109 805 782
Fragments arranged by size:
805 782 109 14
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 601 634 1710
Size: 601 33 1076
Fragments arranged by size:
1076 601 33
BanII G_rGCy'C
Cuts at: 0 15 122 232 254 1710
Size: 15 107 110 22 1456
Fragments arranged by size:
1456 110 107 22 15
BbvCI CC'TCA_GC
Cuts at: 0 1252 1710
Size: 1252 458
BfrBI ATG'CAT
Cuts at: 0 1324 1710
Size: 1324 386
BglI GCCn_nnn'nGGC
Cuts at: 0 997 1710
Size: 997 713
BlpI GC'TnA_GC
Cuts at: 0 1078 1710
Size: 1078 632
Bme1580I G_kGCm'C
Cuts at: 0 15 139 866 1527 1710
Size: 15 124 727 661 183
Fragments arranged by size:
727 661 183 124 15
BmrI ACTGGGnnnn_n'
Cuts at: 0 1233 1343 1507 1671 1710
Size: 1233 110 164 164 39
Fragments arranged by size:
1233 164 164 110 39
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 529 1710
Size: 529 1181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 595 652 1031 1121 1397 1643 1710
Size: 411 184 57 379 90 276 246 67
Fragments arranged by size:
411 379 276 246 184 90 67 57
Bpu10I CC'TnA_GC
Cuts at: 0 1252 1710
Size: 1252 458
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1431 1710
Size: 1431 279
BsaI GGTCTCn'nnnn_
Cuts at: 0 175 862 1568 1710
Size: 175 687 706 142
Fragments arranged by size:
706 687 175 142
BsaAI yAC'GTr
Cuts at: 0 654 1710
Size: 654 1056
BsaHI Gr'CG_yC
Cuts at: 0 143 1710
Size: 143 1567
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 516 546 1484 1514 1710
Size: 516 30 938 30 196
Fragments arranged by size:
938 516 196 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1190 1415 1710
Size: 1190 225 295
Fragments arranged by size:
1190 295 225
BseYI C'CCAG_C
Cuts at: 0 93 1601 1710
Size: 93 1508 109
Fragments arranged by size:
1508 109 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 380 685 1531 1710
Size: 154 226 305 846 179
Fragments arranged by size:
846 305 226 179 154
BsiEI CG_ry'CG
Cuts at: 0 107 1710
Size: 107 1603
BsiHKAI G_wGCw'C
Cuts at: 0 26 232 866 1634 1710
Size: 26 206 634 768 76
Fragments arranged by size:
768 634 206 76 26
BsiWI C'GTAC_G
Cuts at: 0 154 1710
Size: 154 1556
Bsp1286I G_dGCh'C
Cuts at: 0 15 26 122 139 232 254 866 1527
Size: 15 11 96 17 93 22 612 661
Cuts at: 1527 1634 1710
Size: 107 76
Fragments arranged by size:
661 612 107 96 93 76 22 17
15 11
BspHI T'CATG_A
Cuts at: 0 1382 1710
Size: 1382 328
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 556 707 962 1710
Size: 556 151 255 748
Fragments arranged by size:
748 556 255 151
BsrFI r'CCGG_y
Cuts at: 0 49 1710
Size: 49 1661
BssHII G'CGCG_C
Cuts at: 0 62 1710
Size: 62 1648
BssSI C'ACGA_G
Cuts at: 0 859 1702 1710
Size: 859 843 8
Fragments arranged by size:
859 843 8
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1614 1710
Size: 1614 96
BstYI r'GATC_y
Cuts at: 0 210 686 1710
Size: 210 476 1024
Fragments arranged by size:
1024 476 210
BtgI C'CryG_G
Cuts at: 0 1055 1186 1710
Size: 1055 131 524
Fragments arranged by size:
1055 524 131
BtsI GCAGTG_nn'
Cuts at: 0 28 981 993 1505 1710
Size: 28 953 12 512 205
Fragments arranged by size:
953 512 205 28 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 1197 1710
Size: 1197 513
EaeI y'GGCC_r
Cuts at: 0 174 585 1568 1710
Size: 174 411 983 142
Fragments arranged by size:
983 411 174 142
EarI CTCTTCn'nnn_
Cuts at: 0 620 1447 1542 1710
Size: 620 827 95 168
Fragments arranged by size:
827 620 168 95
EcoICRI GAG'CTC
Cuts at: 0 230 1710
Size: 230 1480
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 235 411 595 652 1031 1121 1397 1643
Size: 235 176 184 57 379 90 276 246
Cuts at: 1643 1710
Size: 67
Fragments arranged by size:
379 276 246 235 184 176 90 67
57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 238 1710
Size: 238 1472
EcoO109I rG'GnC_Cy
Cuts at: 0 11 783 1710
Size: 11 772 927
Fragments arranged by size:
927 772 11
HaeII r_GCGC'y
Cuts at: 0 1602 1710
Size: 1602 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 188 220 697 729 1103 1135 1710
Size: 188 32 477 32 374 32 575
Fragments arranged by size:
575 477 374 188 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 1710
Size: 251 1459
MscI TGG'CCA
Cuts at: 0 587 1570 1710
Size: 587 983 140
Fragments arranged by size:
983 587 140
MslI CAynn'nnrTG
Cuts at: 0 205 532 962 986 1513 1710
Size: 205 327 430 24 527 197
Fragments arranged by size:
527 430 327 205 197 24
NcoI C'CATG_G
Cuts at: 0 1055 1186 1710
Size: 1055 131 524
Fragments arranged by size:
1055 524 131
NsiI A_TGCA'T
Cuts at: 0 1326 1710
Size: 1326 384
NspI r_CATG'y
Cuts at: 0 1077 1208 1274 1328 1710
Size: 1077 131 66 54 382
Fragments arranged by size:
1077 382 131 66 54
PasI CC'CwG_GG
Cuts at: 0 1294 1710
Size: 1294 416
PflMI CCAn_nnn'nTGG
Cuts at: 0 1187 1245 1519 1557 1710
Size: 1187 58 274 38 153
Fragments arranged by size:
1187 274 153 58 38
PspOMI G'GGCC_C
Cuts at: 0 11 1710
Size: 11 1699
PstI C_TGCA'G
Cuts at: 0 35 988 1417 1456 1658 1710
Size: 35 953 429 39 202 52
Fragments arranged by size:
953 429 202 52 39 35
SacI G_AGCT'C
Cuts at: 0 232 1710
Size: 232 1478
SapI GCTCTTCn'nnn_
Cuts at: 0 620 1710
Size: 620 1090
SbfI CC_TGCA'GG
Cuts at: 0 1417 1710
Size: 1417 293
SexAI A'CCwGG_T
Cuts at: 0 792 1710
Size: 792 918
SfcI C'TryA_G
Cuts at: 0 31 255 467 984 1001 1413 1452 1654
Size: 31 224 212 517 17 412 39 202
Cuts at: 1654 1710
Size: 56
Fragments arranged by size:
517 412 224 212 202 56 39 31
17
SmaI CCC'GGG
Cuts at: 0 16 125 1710
Size: 16 109 1585
Fragments arranged by size:
1585 109 16
SmlI C'TyrA_G
Cuts at: 0 1446 1710
Size: 1446 264
SphI G_CATG'C
Cuts at: 0 1274 1710
Size: 1274 436
SspI AAT'ATT
Cuts at: 0 757 1710
Size: 757 953
StyI C'CwwG_G
Cuts at: 0 113 588 902 1055 1130 1186 1487 1565
Size: 113 475 314 153 75 56 301 78
Cuts at: 1565 1710
Size: 145
Fragments arranged by size:
475 314 301 153 145 113 78 75
56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 121 895 1710
Size: 121 774 815
Fragments arranged by size:
815 774 121
TatI w'GTAC_w
Cuts at: 0 545 1710
Size: 545 1165
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1484 1516 1710
Size: 1484 32 194
Fragments arranged by size:
1484 194 32
Tth111I GACn'n_nGTC
Cuts at: 0 1015 1710
Size: 1015 695
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1062 1710
Size: 1062 648
XmaI C'CCGG_G
Cuts at: 0 14 123 1710
Size: 14 109 1587
Fragments arranged by size:
1587 109 14
ZraI GAC'GTC
Cuts at: 0 144 1710
Size: 144 1566
Enzymes that do cut:
AatII AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
AvaI BaeI BanII BbvCI BfrBI BglI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I
BspHI BspMI BsrFI BssHII BssSI BstXI BstYI BtgI
BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I MmeI MscI MslI NcoI NsiI NspI
PasI PflMI PspOMI PstI SacI SapI SbfI SexAI
SfcI SmaI SmlI SphI SspI StyI TaqII TatI
TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BamHI BanI
BbeI BbsI BcgI BciVI BclI BglII BmgBI BmtI
BsaBI BsaWI BsmI BsmBI BspEI BsrBI BsrDI BsrGI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PciI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI ScaI SfiI SfoI SgfI SgrAI SnaBI
SpeI SrfI StuI SwaI XbaI XhoI XmnI